衡阳紫色土丘陵坡地植被恢复对土壤微生物功能多样性的影响

doi:10.13287/j.1001-9332.201608.009

应用生态学报 ›› 2016, Vol. 27 ›› Issue (8): 2645-2654.doi: 10.13287/j.1001-9332.201608.009

衡阳紫色土丘陵坡地植被恢复对土壤微生物功能多样性的影响

文东新¹, 杨宁^2*, 杨满元²

¹中南林业科技大学理学院, 长沙 410004;
²湖南环境生物职业技术学院园林学院, 湖南衡阳 421005

收稿日期:2016-01-07 发布日期:2016-08-18
通讯作者: * E-mail: yangning8787@sina.com
作者简介:文东新,男,1966年生,博士,副教授.主要从事植物生态学及恢复生态学研究,发表论文20余篇.E-mail:wendongxin0809@sina.com
基金资助:
本文由湖南省科技厅项目(S2006N332)、湖南省教育厅科学研究项目(12C1057)、湖南省林业科技创新计划项目(XLK201341)、湖南省衡阳市农业科技支撑计划项目(2014KN27)和湖南省普通高校青年骨干教师培养对象项目资助

Effects of re-vegetation on soil microbial functional diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang, Hunan Province, China.

WEN Dong-xin¹, YANG Ning^2*, YANG Man-yuan²

¹College of Science, Central South University of Forestry and Technology, Changsha 410004, China;
²College of Landscape Architecture, Hunan Environmental-Biological Polytechnic College, Hengyang 421005, Hunan, China

Received:2016-01-07 Published:2016-08-18
Contact: * E-mail: yangning8787@sina.com
Supported by:
This paper was supported by Project of Hunan Provincial Science & Technology Department (S2006N332), Scientific Research Project of the Education Department of Hunan Province (12C1057), Forestry Scientific and Technical Innovation Program of Hunan Province (XLK201341), Agricultural Science and Technology Support Project of Hengyang City, Hunan Province (2014KN27) and Project for Cultivating Young Teachers in Colleges and Universities in Hunan Province.

摘要/Abstract

摘要： 以典型湖南省衡阳紫色土丘陵坡地不同植被恢复阶段为研究对象,采用空间代替时间序列的方法,选用立地条件基本相似的草本(狗尾草,GS)、灌草(紫薇-狗尾草,FG)、灌丛(牡荆+剌槐,FX)和乔灌(枫香+苦楝-牡荆,AF)群落阶段,运用Biolog-ECO微平板技术,对4个不同恢复阶段0～10和10～20 cm土层的土壤微生物功能多样性进行研究,探讨植被恢复对土壤微生物功能多样性的影响.结果表明: 植被恢复后土壤微生物群落代谢活性显著升高,同一土层不同恢复阶段AWCD值的大小顺序为乔灌群落＞灌丛群落＞灌草群落＞草本群落,相同恢复阶段不同土层的AWCD值的大小顺序为0～10 cm＞10～20 cm;主成分分析(PCA)表明,灌草群落与灌丛群落具有相似的土壤微生物C源利用方式及代谢功能,而草本群落、乔灌群落具有不同的C源利用方式及代谢功能,在主成分分离中起主要贡献作用的C源是糖类、氨基酸类以及代谢中间产物和次生代谢物;土壤微生物的Shannon物种丰富度指数(H)、Shannon均匀度指数(E)、Simpson优势度指数(D)和McIntosh指数(U)均以乔灌群落最高,灌草群落和灌丛群落次之,草本群落最低;相关分析表明,土壤含水量(SWC)、土壤总有机碳(STOC)、全氮(TN)、全磷(TP)和速效磷(AP)对土壤微生物代谢功能及功能多样性指数有重要影响,脲酶(URE)、磷酸酶(APE)、蔗糖酶(INV)和过氧化氢酶(CAT)活性与土壤微生物代谢功能及功能多样性指数存在显著相关关系.表明植被恢复可使土壤微生物代谢功能增强,土壤微生物繁殖加快、数量增大,从而促进土壤微生物对土壤C源的利用强度.

Abstract: The aim of the study was to explore the effects of re-vegetation on soil microbial functio-nal diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang, Hunan Province, China. By using the spatial series to replace time series, four typical sampling plots, grass (Setaria viridi, GS), frutex and grass (Lagerstroemia indica-Setaria viridi, FG), frutex (Vitex negundo var. cannabifolia+Robinia pseudoacacia, FX), as well as arbor and frutex (Liquidamdar formosana+Melia azedarach-Vitex negundo var. cannabifolia, AF) community were selected to study the soil microbial functional diversity by using the Biolog-ECO micro-plate technique. The four communities in purple soils on sloping-land were similar and denoted four different re-vegetation stages. The results showed that the soil microbial metabolic activity increased after re-vegetation significantly, and the average well color development (AWCD) which represented soil microbial activity and functional diversity followed the order of AF community＞FX community＞FG community＞GS community at different re-vegetation stages, and followed the order of 0-10 cm ＞10-20 cm in different soil layers. Principal component analysis (PCA) identified that FG and FX community had similar C sources utilization mode and metabolic function, and GS and AF community were diffe-rent. The carbohydrates, amino acids, intermediate metabolites, and secondary metabolites were the main carbon sources separating the two principal component factors. The Shannon species richness index (H), Shannon evenness index (E), Simpson dominance index (D), McIntosh index (U) at four re-vegetation stages were the highest in AF community, the second in FG and FX community, and the lowest in GS community. The results of correlation analysis indicated that the content of soil water content (SWC), soil total organic carbon (STOC), total nitrogen (TN), total phospho-rus (TP) and available phosphorus (AP) had important influence on the soil microbial metabolic function and functional diversity indices. There existed significant correlation between the activities of urease (URE), alk-phosphatase (APE), invertase (INV), catalase (CAT) and the soil microbial metabolic function and functional diversity indices. All the results indicated that re-vegetation could enhance the soil microbial metabolic function, which was beneficial to the reproduction of soil micro-organisms, thereby promoting an increase of soil carbon source utilization intensity.

文东新, 杨宁, 杨满元. 衡阳紫色土丘陵坡地植被恢复对土壤微生物功能多样性的影响[J]. 应用生态学报, 2016, 27(8): 2645-2654.

WEN Dong-xin, YANG Ning, YANG Man-yuan. Effects of re-vegetation on soil microbial functional diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang, Hunan Province, China.[J]. Chinese Journal of Applied Ecology, 2016, 27(8): 2645-2654.

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衡阳紫色土丘陵坡地植被恢复对土壤微生物功能多样性的影响

Effects of re-vegetation on soil microbial functional diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang, Hunan Province, China.

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