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应用生态学报 ›› 2016, Vol. 27 ›› Issue (8): 2379-2389.doi: 10.13287/j.1001-9332.201608.030

• 目次 •    下一篇

东北森林两种典型阔叶树种子和幼苗对15N同位素的富集响应

王广臣1,2,3, 宋媛1,2,3, 闫巧玲1,2*, 张金鑫1,2   

  1. 1中国科学院清原森林生态系统观测研究站, 沈阳 110016;
    2中国科学院森林生态与管理重点实验室, 沈阳 110016;
    3中国科学院大学, 北京 100049
  • 收稿日期:2016-02-04 出版日期:2016-08-18 发布日期:2016-08-18
  • 通讯作者: * E-mail: qlyan@iae.ac.cn
  • 作者简介:王广臣,男,1989年生,硕士研究生.主要从事森林培育学研究.E-mail:wangguangchen14@163.com
  • 基金资助:
    本文由国家自然科学基金项目(31222012)和中国科学院青年创新促进会项目(2011158)资助

Accumulation responses of seeds and seedlings to 15N isotope for two typical broadleaved trees in Northeast China.

WANG Guang-chen1,2,3, SONG Yuan1,2,3, YAN Qiao-ling1,2*, ZHANG Jin-xin1,2   

  1. 1Qingyuan Forest CERN, Chinese Academy of Sciences, Shenyang 110016, China;
    2Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    3University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-02-04 Online:2016-08-18 Published:2016-08-18
  • Contact: * E-mail: qlyan@iae.ac.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31222012) and the Youth Innovation Promotion Association of Chinese Academy of Sciences (2011158).

摘要: 以东北森林两种典型的阔叶树种风力传播种子——花曲柳和色木槭种子为研究对象,通过室内15N尿素浸泡试验和温室盆栽试验,设置4个浓度(0、0.05、0.1和0.2 g·L-1)、3个浸泡时间(4、8和12 d)与4个叶期(2、4、6和8叶)处理,研究种子浸泡浓度、浸泡时间和幼苗叶期对种子和幼苗15N富集的影响.结果表明: 浸泡浓度和浸泡时间对两树种种子δ15N值均有显著的正反馈作用,高浓度和长时间(0.2 g·L-1+12 d)更有利于种子15N总量的富集,花曲柳和色木槭种子15N同位素最大富集倍数的浸泡浓度和浸泡时间组合分别为0.1 g·L-1+(4、8 d)和0.05 g·L-1+(4、8 d);δ15N值稀释率随幼苗株高的增加先急剧减少(2~6叶)后趋于稳定,幼苗从8叶开始叶片15N总量降低,表明6叶幼苗更适合追踪幼苗的来源;幼苗叶片δ15N值与种子浸泡浓度、浸泡时间和种子的δ15N值呈显著正相关.花曲柳和色木槭种子及幼苗均能成功富集到15N信号,采用0.1 g·L-1+8 d+6叶组合最适合追踪花曲柳和色木槭种子和幼苗.

Abstract: Two typical broadleaved trees (i.e., Fraxinus rhynchophylla and Acer mono) with wind-dispersed seeds in Northeast China were selected in this study. A method of 15N isotope labeling was used to explore the accumulation responses of seeds and seedlings to 15N-urea soaking concentration (0, 0.05, 0.1 and 0.2 g·L-1), soaking time (4, 8 and 12 days) and leaf stage (2, 4, 6, and 8 leaves). The results showed that 15N-urea soaking concentration and soaking time had significantly positive effects on δ15N values of seeds, i.e., higher 15N-urea concentration and longer period of soaking (0.2 g·L-1+12 d) were contributed to more 15N accumulation of seeds. The maximum multiples of 15N accumulation in F. rhynchophylla seeds and A. mono seeds were observed in 0.1 g·L-1+ (4 d, 8 d) and 0.05 g·L-1+ (4 d, 8 d), respectively. The loss rate of δ15N values decreased markedly from 2 leaves to 6 leaves and then kept relatively stable with the increasing seedling height, and the total δ15N values of seedlings started to decline at the stage of 8 leaves. These results suggested that seedlings with 6 leaves were more suitable for tracking seedling source. The δ15N values in leaves of seedlings were significantly positively correlated with 15N-urea concentration level, soaking time and δ15N values of seeds. Overall, the accumulation of 15N-urea could be found in seeds and seedlings of F. rhynchophylla and A. mono. The combination of 15N-urea concentration (0.1 g·L-1), soaking time (8 d) and leaf stage (6 leaves) was the most suitable for tracking the seeds and seedlings of these two broadleaved trees.