外源γ-氨基丁酸对Ca(NO3)2胁迫下甜瓜幼苗NO3--N同化的影响

doi:10.13287/j.1001-9332.201612.017

应用生态学报 ›› 2016, Vol. 27 ›› Issue (12): 3987-3995.doi: 10.13287/j.1001-9332.201612.017

外源γ-氨基丁酸对Ca(NO₃)₂胁迫下甜瓜幼苗NO₃^--N同化的影响

甄爱^1,2,3, 胡晓辉^1,2,3**, 任文奇^1,2,3, 苏春杰^1,2,3, 靳晓青^1,2,3, 孙先鹏^1,2,3

¹西北农林科技大学园艺学院, 陕西杨凌 712100;
²农业部西北设施园艺工程重点实验室, 陕西杨凌 712100;
³陕西省设施农业工程中心, 陕西杨凌 712100

收稿日期:2016-05-03 出版日期:2016-12-18 发布日期:2016-12-18
通讯作者: * E-mail: hxh1977@163.com
作者简介:甄爱,女, 1986年生, 博士, 讲师. 主要从事设施蔬菜逆境生理生态研究. E-mail: zhenai5263344@163.com
基金资助:
本文由陕西省科技统筹创新工程计划项目(2015KTTSNY03-03)、现代农业产业技术体系建设专项(CARS-25-D-02)和西北农林科技大学博士科研启动基金(Z109021104)资助

Effect of exogenous γ-aminobutyric acid on NO₃^--N assimilation in muskmelon under Ca(NO₃)₂ stress

ZHEN Ai^1,2,3, HU Xiao-hui^1,2,3, REN Wen-qi^1,2,3, SU Chun-jie^1,2,3, JIN Xiao-qing^1,2,3, SUN Xian-peng^1,2,3

¹College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China;
²Key Laboratory of Protected Horticultural Engineering in Northwest, Ministry of Agriculture, Yangling 712100, Shaanxi, China;
³Shaanxi Province Facility Agriculture Engineering Center, Yangling 712100, Shaanxi, China

Received:2016-05-03 Online:2016-12-18 Published:2016-12-18
Contact: * E-mail: hxh1977@163.com
Supported by:
This paper was supported by the Innovation Project of Science and Technology Plan of Shaanxi Province (2015KTTSNY03-03), Special Projiect of China Morden Agricultural Industry System Construction (CARS-25-D-02) and Doctors’ Scientific Research Start-up Foundation of Northwest A&F University (Z109021104).

摘要/Abstract

摘要： 以盐敏感型甜瓜品种‘一品天下208’为试材,用80 mmol·L^-1 Ca(NO₃)₂模拟设施土壤盐渍化,采用深液流水培,研究外源 γ-氨基丁酸(GABA)对Ca(NO₃)₂胁迫下甜瓜幼苗硝态氮(NO₃^--N)同化的影响.结果表明: Ca(NO₃)₂胁迫显著降低了甜瓜幼苗体内硝酸还原酶(NR)、谷氨酰胺合酶(GS)和谷氨酸合酶(GOGAT)活性,增强了谷氨酸脱氢酶(GDH)、谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性,导致铵态氮(NH₄⁺-N)和游离氨基酸含量增加,NO₃^--N和可溶性蛋白质含量下降,植株生长和光合作用受到严重抑制.Ca(NO₃)₂胁迫下,外源喷施GABA有效促进了甜瓜根系对NO₃^--N的吸收及其向地上部的转运,并通过增强NR、GS和GOGAT活性提高了甜瓜幼苗对NH₄⁺的同化力;通过抑制GDH脱氨作用减少了甜瓜幼苗体内NH₄⁺的释放量,从而缓解了盐诱导产生的NH₄⁺-N积累所造成的氨毒害作用;外源喷施GABA也能调节甜瓜组织中氨基酸代谢途径,促进蛋白质的合成.表明外源GABA能增强甜瓜幼苗对NO₃^--N的同化能力,调控氨基酸代谢,进而有效缓解Ca(NO₃)₂胁迫对甜瓜幼苗的盐伤害作用.

Abstract: The effect of exogenous γ-aminobutyric acid (GABA) on NO₃^--N assimilation in muskmelon under Ca(NO₃)₂ stress was investigated in ‘ Yipintianxia 208’, a salt-sensitive melon variety cultured under deep flow hydroponics which simulated soil salinization. The results showed that under Ca(NO₃)₂ stress, the activities of nitrate reductase (NR), glutamate synthetase (GS) and glutamate amino transferase (GOGAT) in muskmelon seedlings were significantly reduced, while the activities of glutamate dehydrogenase (GDH), glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate aminotransferase (GPT) were enhanced, leading to increased contents of NH₄⁺-N and total amino acids, and decreased contents NO₃^--N and soluble protein in muskmelon, which further severely inhibited plant growth and photosynthesis of muskmelon seedlings. Exogenous GABA effectively improved the absorption of NO₃^--N in muskmelon roots and its transportation from root to shoot under Ca(NO₃)₂ stress, and improved NH₄⁺-N assimilation by enhancing NR, GS and GOGAT activities in muskmelon seedlings. Exogenous GABA also reduced NH₄⁺ release by limiting GDH deamination, thus further alleviated the toxication of NH₄⁺-N induced by Ca(NO₃)₂ stress. In addition, foliage spraying of GABA could regulate amino acids metabolic pathways and promote protein synthesis. The results suggested that exogenous GABA could improve NO₃^--N assimilation and regulate amino acids metabolism to alleviate Ca(NO₃)₂ stress damage in muskmelon seedlings.

甄爱, 胡晓辉, 任文奇, 苏春杰, 靳晓青, 孙先鹏. 外源γ-氨基丁酸对Ca(NO₃)₂胁迫下甜瓜幼苗NO₃^--N同化的影响[J]. 应用生态学报, 2016, 27(12): 3987-3995.

ZHEN Ai, HU Xiao-hui, REN Wen-qi, SU Chun-jie, JIN Xiao-qing, SUN Xian-peng. Effect of exogenous γ-aminobutyric acid on NO₃^--N assimilation in muskmelon under Ca(NO₃)₂ stress[J]. Chinese Journal of Applied Ecology, 2016, 27(12): 3987-3995.

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外源γ-氨基丁酸对Ca(NO₃)₂胁迫下甜瓜幼苗NO₃^--N同化的影响

Effect of exogenous γ-aminobutyric acid on NO₃^--N assimilation in muskmelon under Ca(NO₃)₂ stress

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