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应用生态学报 ›› 2017, Vol. 28 ›› Issue (1): 89-95.doi: 10.13287/j.1001-9332.201701.035

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四种野生苔草属植物的耐寒性评价

叶艳然, 王文莉*, 郑成淑, 付德静, 刘慧雯   

  1. 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室, 山东泰安 271018
  • 收稿日期:2016-07-11 修回日期:2016-11-02 发布日期:2017-01-18
  • 通讯作者: *E-mail:wangwenli169@163.com
  • 作者简介:叶艳然,女,1990年生,硕士研究生.主要从事观赏植物逆境生理与生物技术研究.E-mail:yeyanran1990@163.com
  • 基金资助:
    本文由山东省自然科学基金项目(ZR2011CM048)和山东省现代农业产业技术体系创新团队经费(SDAIT-06-07)资助

Evaluation of cold resistance of four wild Carex speices

YE Yan-ran, WANG Wen-li*, ZHENG Cheng-shu, FU De-jing, LIU Hui-wen   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China
  • Received:2016-07-11 Revised:2016-11-02 Published:2017-01-18
  • Contact: *E-mail:wangwenli169@163.com
  • Supported by:
    This work was supported by the Natural Science Foundation of Shandong Province (ZR2011CM048), and the Fund of Shandong Province Modern Agricultural Technology System Innovation Team (SDAIT-06-07)

摘要: 以采自泰山的白颖苔草、披针叶苔草、青绿苔草和低矮苔草为材料,观察越冬期间田间适应性并测定低温胁迫下抗寒相关的生理指标,计算半致死温度(LT50),利用模糊隶属函数法对耐寒性进行综合评价.结果表明: 4种野生苔草属植物均能安全越冬,且翌春恢复生长良好,年绿期达到260~310 d,越冬期间枯叶率为12%~95%;LT50为-18.65~-11.74 ℃.随着低温胁迫的加剧,4种苔草的丙二醛(MDA)含量、游离脯氨酸(Pro)和可溶性蛋白含量总体呈先上升后下降的趋势,可溶性糖含量呈上升趋势.除披针叶苔草表现为整体呈下降趋势外,其他3种苔草的超氧化物歧化酶(SOD)活性均呈先上升后下降的趋势.耐寒性相对较弱的白颖苔草较早地积累MDA、Pro和可溶性糖.可溶性蛋白含量在低温处理后期达到最大值,且苔草的耐寒性越强其峰值越小.在低温处理初期披针叶苔草SOD活性显著高于其他3种苔草.4种苔草属植物的耐寒性强弱为:披针叶苔草>青绿苔草>低矮苔草>白颖苔草.

Abstract: Four wild Carex speices (C. rigescens, C. lancifolia, C. leucochlora and C. humilis) collected from Mount Taishan were used as experimental materials. The adaption across the winter and physiological characteristics resistant to cold stress were investigated, semi-lethal temperature (LT50) was calculated and fuzzy subordination method was used to generally evaluate the Carex resistant to cold stress. The results showed that 4 Carex species all survived in the winter safely and restored well to grow in the following spring. The green period of the species was between 260 d and 310 d, and percentage of the withered leaves was between 12% and 95%, the range of LT50 was from -18.65 ℃ to -11.74 ℃. With intensifying cold stress, the contents of MDA, proline (Pro) and soluble protein increased at first and then decreased, while the soluble sugar content increased with the treatment time. C. rigescens with poor cold tolerance showed the early accumulation of MDA, Pro and soluble sugar. The value of soluble protein peaked at the late stage of low tempe-rature stress, and the Carex with stronger cold-resistance showed the smaller value. The SOD activity in the leaves of C. lancifolia was higher than that of the other three species in the beginning of treatments. The cold resistance of four Carex species was in order of C. lancifolia>C. leucochlora>C. humilis>C. rigescens.