欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2017, Vol. 28 ›› Issue (2): 643-650.doi: 10.13287/j.1001-9332.201702.003

• 研究报告 • 上一篇    下一篇

玉米芯、竹炭及油枯吸附-海藻酸钠包埋施氏假单胞菌PFS-4对二氯喹啉酸的降解

张可1*, 陈强2, 陈伟1, 陈佳1, 格桑3, 罗鸿兵1   

  1. 1四川农业大学土木工程学院, 四川都江堰 611830;
    2四川农业大学资源学院, 四川温江 611130;
    3哈尔滨工业大学市政环境工程学院, 哈尔滨 150090
  • 收稿日期:2016-05-12 出版日期:2017-02-18 发布日期:2017-02-18
  • 通讯作者: * E-mail: zhangke@sicau.edu.cn
  • 作者简介:张可, 女, 1982年生, 硕士. 主要从事环境微生物及市政污水处理研究. E-mail: zhangke@sicau.edu.cn
  • 基金资助:
    本文由国家自然科学基金项目(51278318)、四川省科技支撑计划项目(2013SZ0103,2014NZ0044)和四川省教育厅科研创新团队项目 (16TD0006)资助

Degradation of quinclorac by corncob, bamboo charcoal, canola meal adsorption-sodium alginate immobilized Pseudomonas stutzeri PFS-4

ZHANG Ke1*, CHEN Qiang2, CHEN Wei1, CHEN Jia1, GE Sang3, LUO Hong-bing1   

  1. 1College of Civil Engineering, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China;
    2College of Resource Science & Technology, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;
    3School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.

  • Received:2016-05-12 Online:2017-02-18 Published:2017-02-18
  • Contact: * E-mail: zhangke@sicau.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (51278318), the Sichuan Key Scientific Research & Development Program (2013SZ0103,2014NZ0044), and the Scientific Research Innovation Team Project of Sichuan Provincial Education Department (16TD0006).

摘要: 采用玉米芯、竹炭及油枯吸附-海藻酸钠包埋对分离到的施氏假单胞菌PFS-4进行复合固定.采用正交试验对固定化条件进行优化,研究了固定化菌剂及游离菌体对二氯喹啉酸的降解效果.结果表明: 固定化菌剂制备的最佳条件为:海藻酸钠质量分数为4%、吸附载体比例(玉米芯∶竹炭∶油枯)为1∶2∶1、CaCl2质量分数为3%、交联时间4 h.固定化菌剂在温度为30 ℃、初始pH=7的条件下,经6 d培养后,对浓度为800 mg·L-1的二氯喹啉酸降解率为91.4%,而游离菌体的降解率为72.8%.将游离菌体和固定化菌剂用于实际污水及土壤处理时,固定化菌剂对水中及土壤中二氯喹啉酸去除率仍能分别达到84.2%和74.3%.研究结果表明,载体及其联结方式对土壤中二氯喹啉酸去除产生显著影响,翻动频率与土壤中二氯喹啉酸的去除率呈显著正相关.因此,玉米芯、竹炭及油枯吸附-海藻酸钠复合固定施氏假单胞菌PFS-4对不良环境具有较好的缓冲性能,对二氯喹啉酸污染水体及土壤原位生态修复具有潜力.

Abstract: To enhance the degradation of quinclorac in soil and water, corncob, bamboo charcoal, canola meal and sodium alginate were used as complex carriers to immobilize previously isolated bacterial strain Pseudomonas stutzeri PFS-4. Orthogonal experiment was conducted to optimize immobilization condition, and the degradation efficiency of quinclorac by immobilized bacteria and free bacteria in water and soil were further investigated. The results indicated that the optimal conditions for immobilization were: alginate concentration 4%, proportion of adsorption carrier (corncob: bamboo charcoal: canola meal=1:2:1), calcium chloride 3%, crosslinking time 4 h. Immobilized bacteria and free bacteria could effectively degrade 91.4% and 72.8% of quinclorac (800 mg·L-1) after 6 d inoculation at 30 ℃ and pH 7.0. The removal rates of quinclorac by immobilized bacteria and free bacteria were affected in actual wastewater and soil treatments, but the quinclorac removal rate remained at 84.2% in soil and 74.3% in water, respectively. The results also demonstrated that the carrier and entrapment media significantly affected the quinclorac removal in soil, and the degradation rate of quinclorac in soil was significantly positively correlated with turnover frequency. Therefore, the approach of corncob, bamboo charcoal, canola meal adsorption-sodium alginate immobilized P. stutzeri PFS-4 possesses application potential in in situ remediation of quinclorac contaminated water and soil due to its buffering to adverse conditions.