杨树幼苗非结构性碳水化合物对增加降水和氮添加的响应

doi:10.13287/j.1001-9332.201702.012

摘要/Abstract

摘要： 设置3个水分梯度,即自然降水(W₁)、自然降水增加50%(W₂)和增加100%(W₃)以及4个施氮梯度,即模拟氮沉降添加0(N₁)、5(N₂)、10(N₃)和15(N₄) g N·m^-2·a^-1,研究增加降水和氮添加对杨树幼苗非结构性碳水化合物(NSC)的影响.结果表明: 增加降水和氮添加对杨树幼苗NSC含量具有显著的交互作用.随着降水的增加,N₁水平叶片和枝条可溶性糖含量不变,叶片、枝条、主干、粗根和细根淀粉含量下降;N₂和N₃水平各器官可溶性糖含量下降或保持不变,淀粉含量降低或先升高后降低;N₄水平各器官可溶性糖和淀粉含量升高或先降低后升高.随着氮沉降的增加,W₁处理叶片可溶性糖含量不变,淀粉含量增加,细根可溶性糖含量增加,淀粉含量不变;W₂处理各器官可溶性糖含量不变或先增加后减少,淀粉含量降低或先增加后降低;W₃处理各器官可溶性糖、淀粉和NSC含量均增加.各水分和施氮梯度处理下,杨树幼苗可溶性糖含量为39.1～88.3 mg·g^-1,叶片中最高,细根中最低,淀粉含量为23.3～46.8 mg·g^-1,粗根中最高,细根中最低.

Abstract: To understand the effects of precipitation increase and nitrogen addition on non-structural carbohydrates (NSC) of poplar seedlings, Populus × xiaozhuanica cv. Zhangwu seedlings were grown under a full factorial experimental design of two factors, i.e., three water treatments (W₁,natural precipitation; W₂, natural precipitation increased by 50%; W₃, increased by 100%) and four N fertilization levels (N₁,0 g N·m^-2·a^-1; N₂,5 g N·m^-2·a^-1; N₃,10 g N·m^-2·a^-1; N₄,15 g N·m^-2·a^-1). The results showed that changes in NSC contents in each organ of P. Zhangwu seedlings reflected significant interactions between precipitation increase and nitrogen addition. With the increase of precipitation, under the N₁ level, soluble sugar content was unchanged in leaves and branches, while the starch content decreased in leaves, branches, stems, coarse and fine roots. With the increase of precipitation, the soluble sugar content remained unchanged or declined, while the starch content decreased or firstly increased and then decreased in different organs under the N₂ and N₃ levels. The soluble sugar and starch contents increased or firstly decreased and then increased in different organs under the N₄ level. With the increase of N addition, the soluble sugar content remained unchanged and the starch content increased in leaves, soluble sugar content increased and starch content was unchanged in fine roots in the W₁ treatment. The soluble sugar content remained unchanged or firstly increased and then decreased, and the starch content decreased or firstly increased and then decreased in different organs in the W₂ treatment. The soluble sugar, starch and NSC contents increased in the W₃ treatment. In different precipitation and N addition treatments, the soluble sugar contents of P. Zhangwu seedlings ranged from 39.1 to 88.3 mg·g^-1, with the highest value observed in leaves and the lowest value in fine roots, and the starch content ranged from 23.3 to 46.8 mg·g^-1, with the highest in coarse roots and the lowest in fine roots.

王凯, 雷虹, 夏扬, 于国庆. 杨树幼苗非结构性碳水化合物对增加降水和氮添加的响应[J]. 应用生态学报, 2017, 28(2): 399-407.

WANG Kai, LEI Hong, XIA Yang, YU Guo-qing. Responses of non-structural carbohydrates of poplar seedlings to increased precipitation and nitrogen addition.[J]. Chinese Journal of Applied Ecology, 2017, 28(2): 399-407.

参考文献

[1] Pan Q-M (潘庆民), Han X-G (韩兴国), Bai Y-F (白永飞), et al. Advances in physiology and ecology studies on stored non-structure carbohydrates in plants. Chinese Bulletin of Botany (植物学通报), 2002, 19(1): 30-38 (in Chinese)
[2] Latt CR, Nair PKR, Kang BT. Reserve carbohydrate levels in the boles and structural roots of five multipurpose tree species in a seasonally dry tropical climate. Forest Ecology and Management, 2001, 146: 145-158
[3] Poorter L, Kitajima K. Carbohydrate storage and light requirements of tropical moist and dry forest tree species. Ecology, 2007, 88: 1000-1011
[4] Loewe A, Einig W, Shi L, et al. Mycorrhiza formation and elevated CO₂ both increase the capacity for sucrose synthesis in source leaves of spruce and aspen. New Phytologist, 2000, 145: 565-574
[5] Myers JA, Kitajima K. Carbohydrate storage enhances seedling shade and stress tolerance in a neotropical fo-rest. Journal of Ecology, 2007, 95: 383-395
[6] Ouyang M (欧阳明), Yang Q-P (杨清培), Qi H-Y (祁红艳), et al. A comparison of seasonal dynamics of nonstructural carbohydrates for deciduous and evergreen landscape trees in subtropical region, China. Journal of Nanjing Forestry University (Natural Science) (南京林业大学学报: 自然科学版), 2014, 38(2): 105-110 (in Chinese)
[7] Yin J-J (印婧婧), Guo D-L (郭大立), He S-Y (何思源), et al. Non-structural carbohydrate, N, and P allocation patterns of two temperate tree species in a semi-arid region of Inner Mongolia. Acta Scientiarum Naturalium Universitatis Pekinensis (北京大学学报: 自然科学版), 2009, 45(3): 519-527 (in Chinese)
[8] Yu L-M (于丽敏), Wang C-K (王传宽), Wang X-C (王兴昌). Allocation of non-structural carbohydrates for three temperate tree species in Northeast China. Chinese Journal of Plant Ecology (植物生态学报), 2011, 35(12): 1245-1255 (in Chinese)
[9] Zhang H-Y (张海燕), Wang C-K (王传宽), Wang X-C (王兴昌). Within-crown variation in concentrations of non-structural carbohydrates of five temperate tree species. Acta Ecologica Sinica (生态学报), 2015, 35(19): 6496-6506 (in Chinese)
[10] Wang X (王雪), Luo W-T (雒文涛), Yu Q (庾强), et al. Effects of nutrient addition on nitrogen, phosphorus and non-structural carbohydrates concentrations in leaves of dominant plant species in a semiarid steppe. Chinese Journal of Ecology (生态学杂志), 2014, 33(7): 1795-1802 (in Chinese)
[11] Du Y (杜尧), Han Y (韩轶), Wang C-K (王传宽). The influence of drought on non-structural carbohydrates in the needles and twigs of Laris gmelinii. Acta Ecologica Sinica (生态学报), 2014, 34(21): 6090-6100 (in Chinese)
[12] Dong Y-H (董彦红), Liu B-B (刘彬彬), Zhang X (张旭), et al. Responses of non-structural carbohydrate metabolism of cucumber seedlings to drought stress and doubled CO₂ concentration. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(1): 53-60 (in Chinese)
[13] Li N (李娜), Sun T (孙涛), Mao Z-J (毛子军). Effects of long-term high-temperature stress on the biomass and non-structure carbohydrates of Pinus sylvestris var. mongolica seedlings. Bulletin of Botanical Research (植物研究), 2014, 34(2): 212-218 (in Chinese)
[14] Karam F, Kabalan R, Breidi J, et al. Yield and water-production functions of two durum wheat cultivars grown under different irrigation and nitrogen regimes. Agricultural Water Management, 2009, 96: 603-615
[15] Simpson K, Jackson GE, Grace J. The response of aphids to plant water stress: The case of Myzus persicae and Brassica oleracea var. capitata. Entomologia Experimentalis et Applicata, 2012, 142: 191-202
[16] Jiang D-M (蒋德明), Zhou Q-L (周全来), Alamusa (阿拉木萨), et al. Responses of vegetation productivity in Horqin Sand Land to simulated increased precipitation and nitrogen deposition. Chinese Journal of Ecology (生态学杂志), 2011, 30(6): 1070-1074 (in Chinese)
[17] Song L-N (宋立宁), Zhu J-J (朱教君), Kang H-Z (康宏樟). Response of hydraulic structure parameters and growth of Pinus sylvestris var. mongolica seedling to simulated precipitation gradient. Arid Zone Research (干旱区研究), 2013, 30(6): 1021-1027 (in Chinese)
[18] Long F-L (龙凤玲), Li Y-Y (李义勇), Fang X (方熊), et al. Effects of elevated CO₂ concentration and nitrogen addition on soil carbon stability in southern subtropical experimental forest ecosystems. Chinese Journal of Plant Ecology (植物生态学报), 2014, 38(10): 1053-1063 (in Chinese)
[19] Sparrius LB, Sevink J, Kooijman AM. Effects of nitrogen deposition on soil and vegetation in primary succession stages in inland drift sands. Plant and Soil, 2012, 353: 261-272
[20] Li J, Lin S, Taube F, et al. Above and belowground net primary productivity of grassland influenced by supplemental water and nitrogen in Inner Mongolia. Plant and Soil, 2011, 340: 253-264
[21] Sun Y-Y (孙誉育), Tang B (唐波), Yin C-Y (尹春英), et al. Effects of water and nitrogen coupling on growth of Betula albo-sinensis seedlings and its physiological mechanism. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2015, 21(4): 710-716 (in Chinese)
[22] Jiao J-Y (焦娟玉), Yin C-Y (尹春英), Chen K (陈珂). Effects of soil water and nitrogen supply on the photosynthetic characteristics of Jatropha curcas seedlings. Chinese Journal of Plant Ecology (植物生态学报), 2011, 35(1): 91-99 (in Chinese)
[23] Qiu Q (邱权), Li J-Y (李吉跃), Wang J-H (王军辉), et al. Coupling effects of soil water and fertilizer application on leaf δ¹³C of Catalpa bungei seedlings. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2015, 35(11): 2290-2298 (in Chinese)
[24] Yan X-L (闫小莉), Dai T-F (戴腾飞), Jia L-M (贾黎明), et al. Responses of the fine root morphology and vertical distribution of Populus × euramericana ‘Guariento’ to the coupled effect of water and nitrogen. Chinese Journal of Plant Ecology (植物生态学报), 2015, 39(8): 825-837 (in Chinese)
[25] Qi C-M (齐昌民), Yang Q-L (杨启良), Zhao Y (赵馀), et al. Effects of water and nitrogen on Jatropha curcas morphological characteristics and water use efficiency under salt stress. Chinese Journal of Ecology (生态学杂志), 2016, 35(1): 48-56 (in Chinese)
[26] Guo B-Y (郭丙玉), Gao H (高慧), Tang C (唐诚), et al. Response of water coupling with N supply on maize nitrogen uptake, water and N use efficiency, and yield in drip irrigation condition. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(12):3679-3686 (in Chinese)
[27] Du S-P (杜少平), Ma Z-M (马忠明), Xue L (薛亮). Interactive impact of water and nitrogen on yield, quality of watermelon and use of water and nitrogen in gravel-mulched field. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(12): 3715-3722 (in Chinese)
[28] Li Y (李勇), Wang F (王峰), Sun J-S (孙景生), et al. Coupling effect of water and nitrogen on mechanically harvested cotton with drip irrigation under plastic film arid area of western Inner Mongolia, China. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(3): 845-854 (in Chinese)
[29] Zheng Y-P (郑云普), Wang H-X (王贺新), Lou X (娄鑫), et al. Changes of non-structural carbohydrates and its impact factors in trees: A review. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(4): 1188-1196 (in Chinese)
[30] Würth MKR, Riedl SP, Wright SJ, et al. Non-structural carbohydrate pools in a tropical forest. Oecologia, 2005, 143: 11-24
[31] Shi PL, Körner C, Hoch G. A test of the growth-limitation theory for alpine tree line formation in evergreen and deciduous taxa of the eastern Himalayas. Functional Ecology, 2008, 22: 213-220
[32] Newell EA, Mulkey SS, Wright SJ, et al. Seasonal patterns of carbohydrate storage in four tropical tree species. Oecologia, 2002, 131: 333-342
[33] Bullock SH. Seasonal differences in non-structural carbohydrates in two dioecious monsoon-climate trees. Biotropica, 1992, 24: 140-145
[34] Huo C-F (霍常富), Sun H-L (孙海龙), Wang Z-Q (王政权), et al. Effects of light and nitrogen on growth, carbon and nitrogen metabolism of Fraxinusmandshurica seedlings. Scientia Silvae Sinicae (林业科学), 2009, 45(7): 38-44 (in Chinese)
[35] Jing D-W (井大炜), Xing S-J (邢尚军), Du Z-Y (杜振宇), et al. Effects of drought stress on the growth, photosynthetic characteristics, and active oxygen metabolism of poplar seedlings. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(7): 1809-1816 (in Chinese)
[36] Sun C-L (孙翠玲), Zhu Z-X (朱占学), Wang Z (王珍), et al. Study on the soil degradation of the poplar plantation and the technique to preserve and increase soil fertility. Scientia Silvae Sinicae (林业科学), 1995, 31(6): 506-512 (in Chinese)
[37] Galloway JN, Townsend AR, Erisman JW, et al. Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions. Science, 2008, 320: 889-892
[38] Li L-J (李禄军), Zeng D-H (曾德慧), Yu Z-Y (于占源), et al. Effects of nitrogen addition on grassland species diversity and productivity in Keerqin Sandy Land. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(8): 1838-1844 (in Chinese)
[39] Hoch G, Popp M, Körner C. Altitudinal increase of mobile carbon pools in Pinus cembra suggests sink limitation of growth at the Swiss treeline. Oikos, 2002, 98: 361-374
[40] Buysse J, Merckx R. An improved colorimetric method to quantify sugar content of plant tissue. Journal of Experimental Botany, 1993, 44: 1627-1629
[41] Munns R. Physiological processes limiting plant growth in saline soils: Some dogmas and hypotheses. Plant, Cell and Environment, 1993, 16: 15-24
[42] Su L-W (苏李维), Li S (李胜), Ma S-Y (马绍英), et al. Physiological response of the distribution of non-structural carbohydrates to water stress in wheat. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(6): 1759-1764 (in Chinese)
[43] Ericsson T, Rytter L, Vapaavuori E. Physiology of carbon allocation in trees. Biomass and Bioenergy, 1996, 11: 115-127
[44] Eissenstat DM, Yanai RD. The ecology of root lifespan. Advances in Ecological Research, 1997, 27: 1-60
[45] Jiang S-S (蒋思思), Wei L-P (魏丽萍), Yang S (杨松), et al. Short term responses of photosynthetic pigments and nonstructural carbohydrates to simulated nitrogen deposition in three provenances of Pinus tabuliformis Carr. seedlings. Acta Ecologica Sinica (生态学报), 2015, 35(21): 7061-7070 (in Chinese)
[46] Zhou XB, Zhang YM, Ji XH, et al. Combined effects of nitrogen deposition and water stress on growth and phy-siological responses of two annual desert plants in northwestern China. Environmental and Experimental Botany, 2011, 74: 1-8
[47] McGroddy ME, Daufresne T, Hedin LO. Scaling of C:N:P stoichiometry in forests worldwide: Implications of terrestrial redfield-type ratios. Ecology, 2004, 85: 2390-2401
[48] Litton CM, Ryan MG, Knight DH. Effects of tree density and stand age on carbon allocation patterns in postfire lodgepole pine. Ecological Applications, 2008, 14: 460-475
[49] Körner C. Carbon limitation in trees. Journal of Ecology, 2003, 91: 4-17
[50] Rachmilevitch S, Huang BR, Lambers H. Assimilation and allocation of carbon and nitrogen of thermal and nonthermal Agrostis species in response to high soil temperature. New Phytologist, 2006, 170: 479-490
[51] Imaji A, Seiwa K. Carbon allocation to defense, storage and growth in seedlings of two temperate broad-leaved tree species. Oecologia, 2010, 162: 273-281
[52] Lacointe A, Kajji A, Daudet FA, et al. Mobilization of carbon reserves in young walnut trees. Acta Botanica Gallica, 1993, 140: 435-441
[53] Schädel C, Blöchl A, Richter A, et al. Short-term dynamics of nonstructural carbohydrates and hemicelluloses in young branches of temperate forest trees during bud break. Tree Physiology, 2009, 29: 901-911