Abstract: The research of green development regionalization is the premise and basis of the adaptation to the new situations of “Green Development”, “Demonstrative Pilot Zone of Ecological Civilization” and “Experiment Zone of Ecological Civilization”, which should be transformed from the ecological management model of emergency rescue (mountain governance, river governance, lake governance, and poor governance) to the sustainable development model of reducing pressure and increasing efficiency (rich in mountain, rich in water, rich in people, and strengthening in ecology) in Jiangxi Province. Previous studies of regionalization emphasized ecological protection but neglected green development. Based on the spatial data including terrain, geomorphology, ecology, agriculture, forestry, grassland, wetland, economy and tourism in Jiangxi Province, our research set up an index system including 44 indices, which combined qualitative and quantitative, ecological and economic as well as comprehensive and important factors. Based on this index system, the development condition and direction for each research unit was evaluated. After these evaluations, we divided Jiangxi Province into 4 first-level types, 17 second-level types and 51 third-level types. This research took green development as the goal, the county’s ecological resources and the deve-lopment advantages as the basis, the green development approach of each unit as a breakthrough for the first time. The results of the regionalization were helpful to define the direction of the green development for each county, to guide the optimum utilization of resources and the rational distribution of industries, and to provide scientific basis for the ecological environment protection and sustainable development in Jiangxi Province.