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应用生态学报 ›› 2017, Vol. 28 ›› Issue (9): 2923-2931.doi: 10.13287/j.1001-9332.201709.025

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紫花苜蓿种子萌发及幼苗生理特性对PEG6000模拟渗透势的响应

陈士超, 王猛, 汪季*, 高永, 刘宗奇, 王香   

  1. 内蒙古农业大学沙漠治理学院, 呼和浩特 010011
  • 收稿日期:2017-01-18 出版日期:2017-09-18 发布日期:2017-09-18
  • 通讯作者: * E-mail: wangji1957@163.com
  • 作者简介:陈士超, 男, 1977年生, 博士, 讲师. 主要从事资源环境与生态建设研究. E-mail: chenshichao2005@126.com
  • 基金资助:

    本文由林业公益性行业科研专项(201204205)资助

Response of seed germination and seedling physiological characteristics of Medicago sativa to the simulated osmotic potential of PEG6000.

CHEN Shi-chao, WANG Meng, WANG Ji*, GAO Yong, LIU Zong-qi, WANG Xiang   

  1. College of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010011, China.
  • Received:2017-01-18 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: wangji1957@163.com
  • Supported by:

    This work was supported by the Special Fund for Forest Scientific Research in the Public Welfare (201204205)

摘要: 用不同质量浓度PEG6000(0%、5%、10%、15%、20%、25%、30%,渗透势分别为0、-0.06、-0.17、-0.32、-0.53、-0.79、-1.10 MPa)控制渗透势,研究紫花苜蓿种子萌发及幼苗生理特性对不同渗透势的响应.结果表明: 种子5项萌发指标均随渗透势降低呈先升后降趋势,-0.06 MPa时萌发指数和活力指数最高,-0.17 MPa时发芽率、发芽势和发芽指数最高;幼苗过氧化物酶、超氧化物歧化酶、过氧化氢酶活性和叶绿素含量均随渗透势降低呈先升后降趋势,-0.17 MPa时各指标值最高;叶绿素a/b随渗透势降低呈先降后升趋势,-0.06 MPa时最低;游离脯氨酸、丙二醛含量和相对电导率随渗透势降低而持续升高.渗透势为-0.06~-0.17 MPa时,种子萌发和幼苗生理综合评价结果最优,是最适宜种子萌发的渗透势条件,而当渗透势低于-0.79 MPa时,对种子萌发产生抑制.

Abstract: The responses of seed germination and seedling physiological characteristics of alfalfa to different osmotic potentials were studied with the varied concentrations of PEG6000 (0%, 5%, 10%, 15%, 20%, 25% and 30% corresponding to osmotic potential of 0, -0.06, -0.17, -0.32, -0.53, -0.79 and -1.10 MPa, respectively) in this study. Five indexes of seed germination increased firstly and then decreased with a decrease of osmotic potential. Indexes of germination and vigor were the highest when osmotic potential was -0.06 MPa. Rate, potential and index germination reached the maximum when osmotic potential was -0.17 MPa. Seedling POD, SOD, CAT activities and chlorophyll content increased firstly and then decreased with a decrease of osmotic potential, and indexes reached the maximum when osmotic potential was -0.17 MPa. The ratio of chlorophyll a to chlorophyll b decreased firstly and then increased with a decrease of osmotic potential, and it reached the minimum when osmotic potential was -0.06 MPa. Pro, MDA and REC increased with a decrease of osmotic potential. Seed germination and the comprehensive indexes of seedling physiology were optimal for seed germination when osmotic potential was -0.06--0.17 MPa. Seed germination was restrained when the osmotic potential was lower than -0.79 MPa.