Abstract: Understanding the temporal and spatial dynamics of land use/cover (LUC) can contri-bute to reveal the impacts of climate change and human activities on ecosystems and thus be an important prerequisite for ecosystem management. As an ecologically vulnerable area in China, the Three Gorges Reservoir Area (TGRA) of the Yangtze River presented significant complexity in the response to environmental changes. However, there is a general lack of understanding in the underlying mechanism. In this study, we interpreted the time series remote sensing images derived from the Landsat sensors to map the LUC of the TGRA, and aimed to analyze the long-term changes in the distribution and structure of LUC and elucidate the evolution process of LUC, which could provide a scientific basis to understand the complexity of ecosystem changes and regional ecosystem management in ecologically fragile regions. The results showed that the TGRA presented the significant changes in spatial heterogeneity of LUC structure from 1990 to 2015. Meanwhile, the landscape changed from farmland to woodland (forest and shrubland). Farmland decreased from 66.2% to 40.4%, but woodland increased from 31.3% to 53.5%, buildings and water area increased gradually. Forest gradually spread to the middle of the TGRA, while the buildings presented a scattered expansion. In the altitude zone of 500-1000 m and slope zone of 15°-25°, woodland increased noticeably, and the coniferous forest, mixed forest and shrubland had a higher growth rate. In the study period, due to the urban construction and the various ecological restoration projects in the TGRA, the LUC structure varied with the dam’s impoundment and was characterized by the ecosystem restoration. Ecological restoration projects were helpful to reduce the negative impacts of urban construction and economic development on the environment.