Abstract: To explore the effects of different fulvic acid application rates on ¹⁵N-urea absorption, utilization, residue amount, loss and fruit yield and quality, fifteen-year-old ‘Red General’/ Malus micromalus Makino was selected as experiment material by using the ¹⁵N-labeled tracer technique. Five treatments were established: single urea (CK), combination of urea and fulvic acid with varied rates (NF₁, NF₂, NF₃ and NF₄ represented 75, 150, 300 and 450 kg·hm^-2 fulvic acid application rates, respectively). The main results were as follows: the ¹⁵N derived from fertilizer (Ndff) in root, one-year-old branch and leaf were displayed with an order of NF₃>NF₄>NF₂>NF₁>CK at the fruit maturity stage, and significant difference was observed in Ndff value among the five treatments. The total N content and ¹⁵N absorption amount were highest in NF₃, followed by NF₄, and lowest in CK. Compared with CK, the ¹⁵N utilization rate in NF₁, NF₂, NF₃ and NF₄ was increased by 14.2%, 33.5%, 64.2% and 50.0%, and the ¹⁵N loss rate was decreased by 9.1%, 18.5%, 37.1% and 28.7% respectively. The residue amount of ¹⁵N-urea varied across different treatments, and that in 0-60 cm soil layer under combination of urea and fulvic acid was significantly higher than CK, with the highest value occurred in NF₃, while it was lower than CK in 60-100 cm soil layer. In comparison with CK treatment, the largest increment of yield per plant and net income appeared in NF₃, with an enhancement of 15.8% and 20.2%, respectively. In addition, the fruit hardness, soluble solid and sugar-acid ratio reached the highest value in NF₃. Considering the responses of fruit yield and N utilization efficiency, the suitable fulvic acid application rate was 326.41-350.61 kg·hm^-2.