苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响

doi:10.13287/j.1001-9332.201812.004

应用生态学报 ›› 2018, Vol. 29 ›› Issue (12): 4165-4171.doi: 10.13287/j.1001-9332.201812.004

苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响

刘丽英¹,丁文龙¹,曹雅杰¹,尤鸿基¹,刘珂欣¹,孙中涛¹,毛志泉^2*

¹山东农业大学生命科学学院, 山东泰安 271018;
²山东农业大学园艺科学与工程学院/作物生物学国家重点实验室, 山东泰安 271018

收稿日期:2018-04-16 修回日期:2018-10-08 出版日期:2018-12-20 发布日期:2018-12-20
作者简介:刘丽英,女,1976年生,博士,副教授. 主要从事微生物与宿主间互作研究. E-mail: llylxy@163.com
基金资助:
本文由现代农业产业技术体系建设专项(CARS-28)、山东省重点研发计划项目(2017CXGC0306)和山东省“双一流”项目(SYL2017XTTD15)资助

Effects of antagonistic bacteria B6 against the pathogens of apple replant disease on the biomass of Malus hupehensis Rehd seedlings and soil environment under replanting

LIU Li-ying¹, DING Wen-long¹, CAO Ya-jie¹, YOU Hong-ji¹, LIU Ke-xin¹, SUN Zhong-tao¹, MAO Zhi-quan^2*

¹College of Life Science, Shandong Agricultural University, Tai’an 271018, Shandong, China;
²State Key Laboratory of Crop Biology/College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China

Received:2018-04-16 Revised:2018-10-08 Online:2018-12-20 Published:2018-12-20
Supported by:
This work was supported by Special Fund for Modern Agricultural Industry Technical System Construction (CARS-28), Key Research and Development Program of Shandong Province (2017CXGC0306), and Shandong Province “Double first-rate” Project (SYL2017XTTD15).

摘要/Abstract

摘要： 以苹果连作障碍病原真菌层出镰刀菌、串珠镰刀菌、尖孢镰刀菌和腐皮镰刀菌为靶标菌,通过平板对峙法对分离自苹果根际土壤的细菌进行反复筛选比较,对筛选出的拮抗效果最优的菌株进行形态学、生理生化特征和16S rDNA序列分析鉴定,并于盆栽条件下探讨其菌肥对平邑甜茶幼苗生长及连作土壤环境的影响.结果表明: 菌株B6对上述4种病原真菌的抑菌率最高,分别达到71.8%、70.1%、72.6%、91.5%.经鉴定,菌株B6为甲基营养型芽孢杆菌.盆栽试验表明,与连作处理(CK₁)相比,B6菌肥处理(T)可以不同程度地促进平邑甜茶幼苗生物量的增加,其中地径、鲜质量和干质量分别显著增加18.3%、51.2%;显著提高连作土壤中可培养细菌和放线菌数量,使真菌数量下降为连作土壤的37.7%,促使土壤类型向细菌型转化;显著提高连作土壤中的蔗糖酶、磷酸酶、脲酶和过氧化氢酶的活性,增长率分别为37.3%、24.0%、42.9%、49.4%.表明B6菌肥可以优化苹果连作障碍土壤中可培养微生物群落结构,提高土壤酶活性,增加平邑甜茶幼苗生物量.

Abstract: We isolated strains from the rhizosphere soil of apple trees with replanting disease and evaluated the biological control potential for the pathogens Fusarium proliferatum, F. moniliforme, F. oxysporum, and F. solani. The morphological, physiological and biochemical character, and 16S rDNA sequence of the strain with the highest inhibitory rate were analyzed. The effect of strain biofertilizer on the biomass of Malus hupehensis Rehd. seedlings and soil environment under replanting disease was evaluated in a pot experiment. The results showed that the strain B6 had the strongest antagonistic activity. The inhibitory rate of B6 for F. proliferatum, F. moniliforme, F. oxysporum, Fusarium solani reached 71.8%, 70.1%, 72.6% and 91.5%, respectively. The strain B6 was identified as Bacillus methylotrophicus according to the results of morphological, physiological and biochemical character and 16S rDNA sequence analysis. Compared with the control, the bacterial manure made from the strain B6 enhanced the biomass of Malus hupehensis Rehd. seedlings in replanting soil to different extent. The ground diameter, fresh and dry mass were significantly increased by 18.3%, 49.6% and 51.2%, respectively. The strain B6 dramatically increased the number of cultivable bacteria and actinomyces in replanting soil and reduced the abundance of fungus to 37.7%, which accelerated the conversion of fungal soil to bacterial soil. It also dramatically increased the activities of sucrase, phosphatase, ureaseandcatalase in soil by 37.3%, 24.0%, 42.9% and 49.4%, respectively. In conclusion, the B6 fertilizer could improve the structure of cultivable microbial communities in the continuous cropping soil of apple trees, increase the soil enzyme activity, and enhance the growth of Malus hupehensis seedlings.

刘丽英,丁文龙,曹雅杰,尤鸿基,刘珂欣,孙中涛,毛志泉. 苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响[J]. 应用生态学报, 2018, 29(12): 4165-4171.

LIU Li-ying, DING Wen-long, CAO Ya-jie, YOU Hong-ji, LIU Ke-xin, SUN Zhong-tao, MAO Zhi-quan. Effects of antagonistic bacteria B6 against the pathogens of apple replant disease on the biomass of Malus hupehensis Rehd seedlings and soil environment under replanting[J]. Chinese Journal of Applied Ecology, 2018, 29(12): 4165-4171.

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苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响

Effects of antagonistic bacteria B6 against the pathogens of apple replant disease on the biomass of Malus hupehensis Rehd seedlings and soil environment under replanting

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