Abstract: The increasing nitrogen deposition due to human activities has impacted forest ecosystems to a large extent. The organic carbon and nitrogen released from decomposing litters play an important role in the formation, stability and transformation of soil organic carbon and nitrogen. We collected soil samples from a subtropical evergreen broadleaved forest experiment with nitrogen deposition [control (0), LN (75 kg·hm^-2·a^-1), HN (150 kg·hm^-2·a^-1)] and litter control (litter retained and litter removal) for eight years. After extracted by solution of K₂SO₄, Na₂B₄O₇, Na₄P₂O₇, NaOH, H₂SO₄, Na₂S₂O₄ and HF step by step, carbon and nitrogen in each extraction was analyzed. The results showed that overall most of soil carbon and nitrogen existed in the Humin fraction, accounting for 33.5% of the total carbon and 33.3% of the total nitrogen. The soluble total carbon and nitrogen extracted by Na₂B₄O₇ solution was the highest, followed by NaOH and Na₄P₂O₇ solution. The soluble total carbon, soluble total nitrogen and soluble organic nitrogen of soil extracted by three reagents accounted for 46.2%, 47.9%, and 76.5% of the total extractions, respectively. In addition, nitrogen addition significantly increased carbon and nitrogen content in Na₂S₂O₄ and Humin fractions. Litter removal reduced carbon content in Na₂B₄O₇, H₂SO₄, Na₂S₂O₄ and Humin fractions, and nitrogen content in NaOH, HF and Humin fractions. The nitrogen content in the K₂SO₄ extraction was significantly increased by both litter remained and nitrogen addition. Our results demonstrated that litter and nitrogen added could mutually affect carbon and nitrogen concentration of soil fractions with different chemical stability, with consequences on the process of soil carbon and nitrogen.