石墨烯对植物的生理毒性效应研究进展

doi:10.13287/j.1001-9332.202006.041

摘要/Abstract

摘要： 石墨烯是当前研究最热的碳纳米材料,具有独特的理化特性,在各领域广泛应用。随着石墨烯生产和使用量的不断增大,其不可避免地进入到环境中,给生态环境和人类健康带来风险。阐明石墨烯的潜在毒性效应及其作用机制对于客观评价石墨烯的生态环境健康风险具有重要意义。迄今为止,已有诸多研究报道了石墨烯的植物生理毒理效应。研究表明,石墨烯对植物的生理学响应及其生理变化过程存在影响,涉及萌芽、幼苗生长、氧化应激、光合特性、植物激素和代谢过程等,且多呈浓度效应。今后亟需构建一套被广泛认可的植物石墨烯毒性评价体系,为石墨烯的安全生产和使用提供指导。

Abstract: Graphene is one of the most popular carbon nanomaterials that widely used in many fields due to its unique physical and chemical properties. The expanding production and application of graphene materials has led to their inputs into the environment, with increasing risks of environment and human health. Therefore, elucidating the potential toxic effects of graphene and the related mechanism are of significance to evaluate its ecological risk and bio-safety. To date, many studies have reported the physiotoxicological effects of graphene on plants. Literature showed that graphene had concentration-dependent effects on the physiological response of plants, including seed germination, growth, oxidative stress, photosynthetic characteristics, plant hormones, and metabolic processes. In the future, it is necessary to establish a widely accepted phytotoxicity evaluation system for the safe manufacture and use of graphene.

翁轶能, 蒋楠, 李佳欣, 应祉凝, 都韶婷. 石墨烯对植物的生理毒性效应研究进展[J]. 应用生态学报, 2020, 31(6): 2129-2138.

WENG Yi-neng, JIANG Nan, LI Jia-xin, YING Zhi-ning, DU Shao-ting. Research advances in physiological toxicity of graphene on plants[J]. Chinese Journal of Applied Ecology, 2020, 31(6): 2129-2138.

参考文献 57

[1]	Novoselov KS, Geim AK, Morozov SV, et al. Electric field effect in atomically thin carbon films. Science, 2004, 306: 666-669
[2]	Mahmoudi T, Wang Y, Hahn YB. Graphene and its derivatives for solar cells application. Nano Energy, 2018, 47: 51-65
[3]	Scott A. Graphene’s global race to market. Chemical & Engineering News, 2016, 94: 28-33
[4]	Ren W, Cheng H. The global growth of graphene. Nature Nanotechnology, 2014, 9: 726-730
[5]	任文杰, 滕应. 石墨烯的环境行为及其对环境中污染物迁移归趋的影响. 应用生态学报, 2014, 25(9): 2723-2732 [Ren W-J, Teng Y. Environmental behavior of graphene and its effect on the transport and fate of pollutants in environment. Chinese Journal of Applied Ecology, 2014, 25(9): 2723-2732]
[6]	刘尚杰. 石墨烯对水稻种子萌发及幼苗生长的影响. 硕士论文. 荆州: 长江大学, 2013 [Liu S-J. The Effect of Graphene on the Germination and Seedling Growth in Rice. Master Thesis. Jingzhou: Yangtze University, 2013]
[7]	Liu S, Wei H, Li Z, et al. Effects of graphene on germination and seedling morphology in rice. Journal of Nanoscience and Nanotechnology, 2015, 15: 2695-2701
[8]	Caklr Y, Caklr OUC. Investigation of graphene phytotoxi-city in the germination stage of wheat and barley. IEEE 2015 Fourth International Conference on Agro-Geoinformatics. Istanbul, Turkey, 2015: 19-22
[9]	Caklr Y, Caklr OUC. Phytotoxicity of graphene in tomatoes and bean. IEEE 2016 Fifth International Conference on Agro-Geoinformatics. Tianjin, China, 2016: 1-4
[10]	Anjum NA, Singh N, Singh MK, et al. Single-bilayer graphene oxide sheet impacts and underlying potential mechanism assessment in germinating faba bean (Vicia faba L.). Science of the Total Environment, 2014, 472: 834-841
[11]	吴金海, 焦靖芝, 谢伶俐, 等. 氧化石墨烯处理对甘蓝型油菜生长发育的影响. 基因组学与应用生物学, 2015, 34(12): 2738-2742 [Wu J-H, Jiao J-J, Xie L-L, et al. Effects of grapheme oxide on growth and deve-lopment of Brassica napus L. Genomics and Applied Biology, 2015, 34(12): 2738-2742]
[12]	王晓静, 赵树兰, 多立安. 氧化石墨烯拌种对高羊茅种子萌发与幼苗生长的影响. 种子, 2018, 37(4): 1-4 [Wang X-J, Zhao S-L, Duo L-A. Effect of seed dressing with graphene oxide on seed germination and seedling growth of Festuca arundinacea. Seeds, 2018, 37(4): 1-4]
[13]	Begum P, Ikhtiari R, Fugetsu B. Graphene phytotoxicity in the seedling stage of cabbage, tomato, red spinach, and lettuce. Carbon, 2011, 49: 3907-3919
[14]	Cheng F, Liu Y, Lu G, et al. Graphene oxide modulates root growth of Brassica napus L. and regulates ABA and IAA concentration. Journal of Plant Physiology, 2016, 193: 57-63
[15]	常海伟. 石墨烯吸附多氯联苯及其对植物毒性的影响研究. 硕士论文. 贵阳: 贵州大学, 2016 [Chang H-W. Adsorption of Polychlorinated Biphenyls by Graphene and Its Effects on Plant Toxicity. Master Thesis. Guiyang: Guizhou University, 2016]
[16]	Yin L, Wang Z, Wang S, et al. Effects of graphene oxide and/or Cd2+ on seed germination, seedling growth, and uptake to Cd2+ in solution culture. Water, Air & Soil Pollution, 2018, 229: 151, doi: 10.1007/s11270-018-3809-y
[17]	Vochita G, Oprica L, Gherghel D, et al. Graphene oxide effects in early ontogenetic stages of Triticum aestivum L. seedlings. Ecotoxicology and Environmental Safety, 2019, 181: 345-352
[18]	He Y, Hu R, Zhong Y, et al. Graphene oxide as a water transporter promoting germination of plants in soil. Nano Research, 2018, 11: 1928-1937
[19]	Zhang M, Gao B, Chen J, et al. Effects of graphene on seed germination and seedling growth. Journal of Nano-particle Research, 2015, 17: 78, doi:10.1007/s11051-015-2885-9
[20]	Morpeth DR, Hall AM. Microbial enhancement of seed germination in Rosa corymbifera ‘Laxa’. Seed Science Research, 2000, 10: 489-494
[21]	Anjum NA, Singh N, Singh MK, et al. Single-bilayer graphene oxide sheet tolerance and glutathione redox system significance assessment in faba bean (Vicia faba L.). Journal of Nanoparticle Research, 2013, 15: 1770, doi: 10.1007/s11051-013-1770-7
[22]	Hu X, Lu K, Mu L, et al. Interactions between graphene oxide and plant cells: Regulation of cell morpho-logy, uptake, organelle damage, oxidative effects and metabolic disorders. Carbon, 2014, 80: 665-676
[23]	Mohanta TK, Bashir T, Hashem A, et al. Early events in plant abiotic stress signaling: Interplay between cal-cium, reactive oxygen species and phytohormones. Journal of Plant Growth Regulation, 2018, 37: 1033-1049
[24]	Gao M, Song Z. Toxicity of cadmium to wheat seedling roots in the presence of graphene oxide. Chemosphere, 2019, 233: 9-16
[25]	Khodakovskaya MV, De Silva K, Biris AS, et al. Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano, 2012, 6: 2128-2135
[26]	Zhao S, Wang Q, Zhao Y, et al. Toxicity and translocation of graphene oxide in Arabidopsis thaliana. Environmental Toxicology and Pharmacology, 2015, 39: 145-156
[27]	Jiao J, Cheng F, Zhang X, et al. Preparation of graphene oxide and its mechanism in promoting tomato roots growth. Journal of Nanoscience and Nanotechnology, 2016, 16: 4216-4223
[28]	常海伟, 任文杰, 刘鸿雁, 等. 磺化石墨烯对小麦幼苗生长及生理生化指标的影响. 生态毒理学报, 2015, 10(4): 123-128 [Chang H-W, Ren W-J, Liu H-Y, et al. Effect of sulfonated graphene on seedling growth and physiological and biochemical indices of wheat. Asian Journal of Ecotoxicology, 2015, 10(4): 123-128]
[29]	Zhang P, Zhang R, Fang X, et al. Toxic effects of graphene on the growth and nutritional levels of wheat (Triticum aestivum L.): Short- and long-term exposure studies. Journal of Hazardous Materials, 2016, 317: 543-551
[30]	王子英. 石墨烯-磺胺嘧啶单一及复合污染对小麦的毒性效应研究. 硕士论文. 新乡: 河南师范大学, 2017 [Wang Z-Y. Toxic Effects of Single and Combined Pollution of Graphene-Sulfadiazine on Wheat. Master Thesis. Xinxiang: Henan Normal University, 2017]
[31]	Horie T, Kaneko T, Sugimoto G, et al. Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots. Plant and Cell Physiology, 2011, 52: 663-675
[32]	Zhou Q, Hu X. Systemic stress and recovery patterns of rice roots in response to graphene oxide nanosheets. Environmental Science & Technology, 2017, 51: 2022-2030
[33]	彭霄鹏. 杨树木质素合成基因C3H与HCT表达下调对细胞壁组成与结构的影响. 博士论文. 北京: 北京林业大学, 2015 [Peng X-P. Effects on Cell Wall Composition and Structure by Down-regulation of Monolignol Biosynthetic Genes C3H and HCT in Populus alba × P. glandulosa. PhD Thesis. Beijing: Beijing Forestry University, 2015]
[34]	Zhao J, Wang Z, White JC, et al. Graphene in the aquatic environment: Adsorption, dispersion, toxicity and transformation. Environmental Science & Technology, 2014, 48: 9995-10009
[35]	Mesaricˇ T, Sepcˇicˇ K, Piazza V, et al. Effects of nano carbon black and single-layer graphene oxide on settlement, survival and swimming behaviour of Amphibalanus amphitrite larvae. Chemistry and Ecology, 2013, 29: 643-652
[36]	Zheng H, Ma R, Gao M, et al. Antibacterial applications of graphene oxides: Structure-activity relationships, molecular initiating events and biosafety. Science Bulletin, 2018, 63: 133-142
[37]	Ouyang S, Hu X, Zhou Q. Envelopment-internalization synergistic effects and metabolic mechanisms of graphene oxide on single-cell Chlorella vulgaris are depen-dent on the nanomaterial particle size. ACS Applied Materials & Interfaces, 2015, 7: 18104-18112
[38]	Anjum NA, Ahmad I, Mohmood I, et al. Modulation of glutathione and its related enzymes in plants’ responses to toxic metals and metalloids: A review. Environmental and Experimental Botany, 2012, 75: 307-324
[39]	Rico CM, Hong J, Morales MI, et al. Effect of cerium oxide nanoparticles on rice: A study involving the antioxidant defense system and in vivo fluorescence imaging. Environmental Science & Technology, 2013, 47: 5635-5642
[40]	Chen J, Yang L, Li S, et al. Various physiological response to graphene oxide and amine-functionalized graphene oxide in wheat (Triticum aestivum). Molecules, 2018, 23: 1104, doi: 10.3390/molecules23051104
[41]	Yuen S, Ma C, Lin Y, et al. Preparation, morphology and properties of acid and amine modified multiwalled carbon nanotube/polyimide composite. Composites Science and Technology, 2007, 67: 2564-2573
[42]	赵圣青. 基于拟南芥的氧化石墨烯毒性效应与转运研究. 硕士论文. 南京: 南京农业大学, 2015 [Zhao S-Q. Toxicity and Translocation of Graphene Oxide in Arabidopsis. Master Thesis. Nanjing: Nanjing Agricultural University, 2015]
[43]	Begum P, Fugetsu, B. Induction of cell death by graphene in Arabidopsis thaliana (Columbia ecotype) T87 cell suspensions. Journal of Hazardous Materials, 2013, 260: 1032-1041
[44]	Nowack B, Bucheli TD. Occurrence, behavior and effects of nanoparticles in the environment. Environmental Pollution, 2007, 150: 5-22
[45]	陈凌云. 石墨烯纳米材料在植物体内的分布、毒性及其环境生态效应研究. 硕士论文. 成都: 西南民族大学, 2018 [Chen L-Y. Distribution, Toxicity and Environmental Ecological Effects of Graphene Nanomaterials in Plants. Master Thesis. Chengdu: Southwest Minzu University, 2018]
[46]	Chen L, Wang C, Yang S, et al. Chemical reduction of graphene enhances in vivo translocation and photosynthetic inhibition in pea plants. Environmental Science: Nano, 2019, 6: 1077-1088
[47]	Hu X, Ouyang S, Mu L, et al. Effects of graphene oxide and oxidized carbon nanotubes on the cellular division, microstructure, uptake, oxidative stress, and metabolic profiles. Environmental Science & Technology, 2015, 49: 10825-10833
[48]	Chen L, Wang C, Li H, et al. Bioaccumulation and toxi-city of 13C-skeleton labeled graphene oxide in wheat. Environmental Science & Technology, 2017, 51: 10146-10153
[49]	Jiao J, Yuan C, Wang J, et al. The role of graphene oxide on tobacco root growth and its preliminary mechanism. Journal of Nanoscience and Nanotechnology, 2016, 16: 12449-12454
[50]	Lin X, Chen L, Hu X, et al. Toxicity of graphene oxide to white moss Leucobryum glaucum. RSC Advances, 2017, 7: 50287-50293
[51]	张鹏. 石墨烯对植物的毒性效应及机制研究. 硕士论文. 杭州: 浙江工商大学, 2015 [Zhang P. Mechanisms of Graphene Toxicity to Plants. Master Thesis. Hangzhou: Zhejiang Gongshang University, 2015]
[52]	Wang Q, Zhao S, Zhao Y, et al. Toxicity and translocation of graphene oxide in Arabidopsis plants under stress conditions. RSC Advances, 2014, 4: 891-901
[53]	宋长春, 王毅勇. 湿地生态系统土壤温度对气温的响应特征及对CO2排放的影响. 应用生态学报, 2006, 17(4): 625-629 [Song C-C, Wang Y-Y. Responses of soil temperature in wetland ecosystem to air temperature and their effects on CO2 emission. Chinese Journal of Applied Ecology, 2006, 17(4): 625-629]
[54]	Chen L, Yang S, Liu Y, et al. Toxicity of graphene oxide to naked oats (Avena sativa L.) in hydroponic and soil cultures. RSC Advances, 2018, 8: 15336-15343
[55]	Shen S, Liu Y, Wang F, et al. Graphene oxide regulates root development and influences IAA concentration in rice. Journal of Plant Growth Regulation, 2019, 38: 241-248
[56]	Weng Y, You Y, Lu Q, et al. Graphene oxide exposure suppresses nitrate uptake by roots of wheat seedlings. Environmental Pollution, 2020, 262: 114224, doi: 10.1016/j.envpol.2020.114224
[57]	Huang C, Xia T, Niu J, et al. Transformation of 14C-labeled graphene to 14CO2 in the shoots of a rice plant. Angewandte Chemie, 2018, 130: 9907-9911