桑天牛虫体提取物的成分及其在两性通讯中的作用

doi:10.13287/j.1001-9332.202010.031

应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3267-3272.doi: 10.13287/j.1001-9332.202010.031

桑天牛虫体提取物的成分及其在两性通讯中的作用

徐昭焕¹, 廖为财², 刘伟¹, 王广利^1*

¹江西农业大学农学院, 南昌 330045;
²赣州市植保植检局, 江西赣州 341000

收稿日期:2019-12-15 接受日期:2020-07-01 出版日期:2020-10-15 发布日期:2021-04-15
通讯作者: * E-mail: wgl_2002@126.com
作者简介:徐昭焕, 女, 1984年生, 博士, 讲师。主要从事农业昆虫与害虫防治研究。E-mail: hzzhaohuan@163.com
基金资助:
国家自然科学基金项目(31060116)资助

Chemical composition of body extracts from Apriona germari and its function in sexual communication

XU Zhao-huan¹, LIAO Wei-cai², LIU Wei¹, WANG Guang-li^1*

¹School of Agricultural Science, Jiangxi Agricultural University, Nanchang 330045, China;
²Plant Protection and Quarantine Bureau of Ganzhou, Ganzhou 341000, Jiangxi, China

Received:2019-12-15 Accepted:2020-07-01 Online:2020-10-15 Published:2021-04-15
Contact: * E-mail: wgl_2002@126.com
Supported by:
National Natural Science Foundation of China (31060116).

摘要/Abstract

摘要： 为明确桑天牛成虫释放的化学信息素成分及其在两性通讯中的作用,利用气质联用仪(GC-MS)分析了桑天牛整体及其腹末组织提取物的化学成分,采用Y型嗅觉仪测定雌雄成虫对5种主要提取物标准品的嗅觉反应,以及雌雄虫对涂抹标准品的洗脱同性与异性成虫的接触反应。结果表明: 桑天牛雌雄成虫整体及其腹末组织提取物化学成分主要为10个碳以上的烷烃和烯烃,其中顺-9-二十三碳烯含量最高,其次为二十七烷、二十九烷、十九碳烯、二十八烷和9-己基-十七烷等,以及少量醛类和酯类物质。嗅觉行为反应结果显示,二十九烷对雌雄虫的引诱作用显著,二十七烷仅对雌虫有显著的引诱作用,而十九碳烯则对雌成虫产生显著的驱避作用,1-二十二碳烯和顺-9-二十三碳烯对桑天牛雌雄虫作用不明显。接触反应结果显示,雄虫对涂抹1-二十二碳烯、二十七烷和二十九烷的洗脱成虫表现出明显的求偶行为,而雌虫仅对涂抹二十九烷的洗脱成虫表现出明显的求偶行为。综合分析发现,1-二十二碳烯、二十七烷和二十九烷是桑天牛性信息素的重要组分,在两性通讯中起重要作用。

关键词: 桑天牛, 信息化学物质, 气相色谱-质谱联用, Y型嗅觉仪, 接触反应

Abstract: To elucidate the composition of semiochemicals of Apriona germari and its function in sexual communication, GC-MS was used to detect the composition of semiochemicals of the overall body and the end abdominal tissue extracts in A. germari. Y-tube olfactometer was used to determine the olfactory response of adult female and male to the standard compounds of the five main extracts. The contact reaction test with male and female adults was performed to the eluted adults that smeared tandard compounds. The results showed that the main ingredients of semiochemicals were alkanes and alkenes with more than 10 carbons. Concentration of (Z)-9-Tricosene was the highest, followed by heptacosane, nonacosane, nonadecene, octacosane, 9-Hexylheptadecane, aldehyde, and ester. Results of the olfactory reaction showed that nonacosane had a significant attractivity to both male and female adults, and that heptacosane had a significant attractivity only to female adults. Nonadecene had a extremely significant repellent activity to female adults. 1-docosene and (Z)-9-Tricosene had no evident role to the male and female adults. Results of the contact test showed that male adults had the strongest courtship responses to the eluted adults with 1-docosene, heptacosane and nonacosane. Female adults had the strongest courtship responses to the eluted adults with nonacosane. Our results indicated that 1-docosene, heptacosane, and nonacosane were important component of the sex pheromone of A. germari, which played an important role in the sexual communication.

Key words: Apriona germari, semiochemicals, GC-MS, Y-tube olfactometer, contact test

徐昭焕, 廖为财, 刘伟, 王广利. 桑天牛虫体提取物的成分及其在两性通讯中的作用[J]. 应用生态学报, 2020, 31(10): 3267-3272.

XU Zhao-huan, LIAO Wei-cai, LIU Wei, WANG Guang-li. Chemical composition of body extracts from Apriona germari and its function in sexual communication[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3267-3272.

参考文献

[1] 黄大庄, 严俊杰, 王志刚, 等. 桑天牛研究. 北京: 科学出版社, 2015: 9-10 [Huang D-Z, Yan J-J, Wang Z-G, et al. Study of Apriona germari. Beijing: Science Press, 2015: 9-10]
[2] 张琳, Wang B, 许志春. 桑科中4种桑天牛寄主植物的挥发物成分研究. 生态学报, 2011, 31(24): 7479-7485 [Zhang L, Wang B, Xu Z-C. Volatile constituents of four moraceous host plants of Apriona germari. Acta Ecologica Sinica, 2011, 31(24): 7479-7485]
[3] 王薇, 李继泉, 崔建州, 等. 桑天牛卵啮小蜂雌蜂对茉莉酸诱导的桑枝挥发物的触角电位和嗅觉反应. 昆虫学报, 2017, 60(9): 41-48 [Wang W, Li J-Q, Cui J-Z, et al. Electroantennographic and olfactory responses of female wasps of Aprostocetus prolixus (Hymenoptera: Eulophidae) to volatiles from Morusalba branches induced by jasmonic acid. Acta Entomologica Sinica, 2017, 60(9): 41-48]
[4] 李会平, 黄大庄, 杜邵华, 等. 不同基质传代白僵菌对桑天牛幼虫的侵染力与寄主血淋巴酚氧化酶活性的关系. 农药学学报, 2008, 10(2): 221-225 [Li H-P, Huang D-Z, Du S-H, et al. Relationship between the infectivity of different subculturing isolatesof Beauveria bassiana on Apriona germari larvae and the phenoloxidase activity in hemolymph of Apriona germari larvae. Chinese Journal of Pesticide Science, 2008, 10(2): 221-225]
[5] Li HP, Han X, Zhao YQ. Potential use of Beauveria bassiana in combination with Scleroderma guani for improved control of Apriona germari. Canadian Journal of Forest Research, 2016, 47: 10-15
[6] Hanks LM, Millar JG. Sex and aggregation-sex pheromones of Cerambycid beetles: Basic science and practical applications. Journal of Chemical Ecology, 2016, 42: 631-654
[7] Millar JG, Hanks LM. Chemical ecology of ceramby-cids// Wang Q, ed. Cerambycidae of the world: Biology and pest management. Boca Raton, FL, USA: CRC Press/Taylor & Francis, 2017: 161-196
[8] 雷朝亮, 荣秀兰. 普通昆虫学. 北京: 中国农业出版社, 2003: 287-297 [Lei C-L, Rong X-L. General Entomology. Beijing: China Agriculture Press, 2003: 287-297
[9] 王安佳, 张开心, 梅向东, 等. 昆虫性信息素及其类似物干扰昆虫行为的机理和应用研究进展. 农药学学报, 2018, 20(4): 425-438 [Wang A-J, Zhang K-X, Mei X-D, et al. Research progress on mechanisms and applications of insect mating behavior disruption by insect sex pheromones and analogues. Chinese Journal of Pesticide Science, 2018, 20(4): 425-438]
[10] Svacha P, Lawrence JF. Cerambycidae Latreille, 1802// Leschen RAB, Beutel RG, eds. Handbook of Zoology: Arthropoda: Insecta: Coleoptera, Beetles. Vol. 3: Morphology and Systematics (Phytophaga). Berlin/Boston: Walter de Gruyter, 2014: 77-177
[11] 伍苏然, 熊国如, 蔡文伟, 等. 信息素在天牛行为中的作用研究进展. 甘蔗糖业, 2018(2): 51-55 [Wu S-R, Xiong G-R, Cai W-W, et al. Advances in the studies on semiochemicals in Cerambycid beetles. Sugarcane and Canesugar, 2018(2): 51-55]
[12] 江望锦, 嵇保中, 刘曙雯, 等. 天牛成虫信息素及嗅觉感受机制研究进展. 昆虫学报, 2005, 48(3): 427-436 [Jiang W-J, Ji B-Z, Liu S-W, et al. Advances in the studies on semiochemicals and olfactory receptor mechanism in adults of cerambycid beetles. Acta Entomologica Sinica, 2005, 48(3): 427-436]
[13] Iwabuchi K. Mating behavior of Xylotrechus pyrrhoderus Bates (Coleoptera: Cerambycidae). Ⅰ. Behavioral sequences and existence of the male sex pheromone. Applied Entomology and Zoology, 1982, 17: 494-500
[14] Barata E, Mustaparta H, Pickett JA, et al. Encoding of host and non-host plant odours by receptor neurones in the eucalyptus woodborer, Phoracantha semipunctata (Coleoptera: Cerambycidae). Journal of Comparative Physiology A, 2002, 188: 121-133
[15] 毕拥国, 郭丽, 王志刚, 等. 桑天牛对5种植物源挥发性化合物及其混合物的触角电位和行为反应. 蚕业科学, 2017, 43(1): 32-38 [Bi Y-G, Guo L, Wang Z-G, et al. Electroantennogram and behavioral responses of Apriona germari to five plant volatiles and their mixtures. Acta Sericologica Sinica, 2017, 43(1): 32-38]
[16] 马艳, 史黎央, 赵艺, 等. 不同危害状态下寄主山核桃挥发物成分的比较及桑天牛对其组分的GC-EAD和行为反应. 昆虫学报, 2018, 61(5): 574-584 [Ma Y, Shi L-Y, Zhao Y, et al. Comparison of volatiles released from the host Juglans mandshurica in different damaged states and the GC-EAD and behavioral responses of Apriona germari (Coleoptera: Cerambycidae) to these volatiles. Acta Entomologica Sinica, 2018, 61(5): 574-584]
[17] Dunn E, Hough-Goldstein J, Hanks LM, et al. Range of attraction of pheromone lures and dispersal behavior of Cerambycid beetles. Annals of the Entomological Society of America, 2016, 109: 872-880
[18] Allison JD, Borden JH, Seybold SJ. A review of the chemical ecology of the Cerambycidae (Coleoptera). Chemoecology, 2004, 14: 123-150
[19] Allison JD, Mckenney JL, Millar JG, et al. Response of the woodborers Monochamus carolinensis and Monocha-mus titillator (Coleoptera: Cerambycidae) to known cerambycid pheromones in the presence and absence of the host plant volatile α-pinene. Environmental Entomo-logy, 2012, 41: 1587-1596
[20] Agnello AM, Loizos L, Dan G. A new pheromone for prionus root-boring beetles. New York Fruit Quarterly, 2011, 19: 17-20
[21] Jayarama MGV, D'Souza MV, Naidu R, et al. Sex pheromone of coffee white stemborer for monitoring and controlis on the anvil. Bulletin of the India Coffee Board, 1997, 62: 15-16
[22] Boyer FD, Malosse C, Zagatti P, et al. Identification and synthesis of vesperal, the female sex pheromone of the longhorn beetle Vesperus xatarti. Bulletin de la Societe Chimique de France, 1997, 134: 757-764
[23] Zhang AJ, James E, Oliver K, et al. Evidence for contact sex recognition pheromone of the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae). Naturwissenschaften, 2003, 90: 410-413
[24] Chang H, Liu Y, Ai D, et al. A Pheromone antagonist regulates optimal mating time in the moth, Helicoverpa armigera. Current Biology, 2017, 27: 1610-1615
[25] 朱宁, 张冬勇, 吴利平, 等. 聚集信息素和寄主植物挥发物对光肩星天牛和星天牛的引诱作用. 昆虫学报, 2017, 60(4): 421-430 [Zhu N, Zhang D-Y, Wu L-P, et al. Attractiveness of aggregation pheromones and host plant volatiles to Anoplophora glabripennis and A. chinensis (Coleoptera: Cerambycidae). Acta Entomolo-gica Sinica, 2017, 60(4): 421-430]

桑天牛虫体提取物的成分及其在两性通讯中的作用

Chemical composition of body extracts from Apriona germari and its function in sexual communication

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