铁胁迫下大豆光合和磷/铁性状对磷素的响应

doi:10.13287/j.1001-9332.202105.025

应用生态学报 ›› 2021, Vol. 32 ›› Issue (5): 1768-1776.doi: 10.13287/j.1001-9332.202105.025

铁胁迫下大豆光合和磷/铁性状对磷素的响应

赵婧, 孟凡钢, 于德彬, 张鸣浩, 饶德民, 丛博韬, 闫晓艳, 张伟^*, 邱强

吉林省农业科学院大豆研究所/大豆国家工程研究中心, 长春 130033

收稿日期:2020-11-03 接受日期:2021-02-21 出版日期:2021-05-15 发布日期:2021-11-15
通讯作者: *E-mail: zw.0431@163.com
作者简介:赵婧,女,1984年生,硕士研究生。主要从事大豆栽培生理生态研究。E-mail:zhao114434260@163.com
基金资助:
国家重点研发计划项目(2018YFD1000905)和吉林省现代农业产业技术示范推广项目(2020-004)资助

Responses of photosynthesis and P/Fe traits to P application in soybean under stress of low Fe.

ZHAO Jing, MENG Fan-gang, YU De-bin, ZHANG Ming-hao, RAO De-min, CONG Bo-tao, YAN Xiao-yan, ZHANG Wei^*, QIU Qiang

National Engineering Research Center of Soybean/Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China

Received:2020-11-03 Accepted:2021-02-21 Online:2021-05-15 Published:2021-11-15
Contact: *E-mail: zw.0431@163.com
Supported by:
National Key Research and Development Program of China (2018YFD1000905) and the Demonstration and Popularization of Modern Agricultural Industrial Technology in Jilin Province (2020-004).

摘要/Abstract

摘要： 为了明确低铁胁迫下磷素用量对大豆光合和磷/铁性状的影响及基因型差异,为磷、铁肥的合理施用提供理论依据,以前期筛选的6个磷高效基因型和6个磷低效基因型大豆为供试材料,设4个P∶Fe配比处理,分别为0∶30、30∶30、150∶30和300∶30(μmol·L^-1),对大豆叶绿素荧光特性和磷、铁利用率进行了测定,利用单株粒重建立逐步回归方程并进行通径分析,通过因子得分综合评价磷高效和磷低效基因型对不同P∶Fe处理的响应。结果表明: 基因型效应、P∶Fe处理效应和两者互作效应对始花期(R1)光系统Ⅱ的相对电子传递速率(ETR)、光系统Ⅱ吸收的能量用于耗散为热量的比例(NPQ)、光系统Ⅱ吸收的能量用于进行光化学反应的比例(qL)的影响均达显著水平。典型相关分析表明,磷高效大豆基因型完熟期(R8)籽粒磷利用率与R1期光合速率呈负相关;磷低效大豆基因型R8期籽粒铁利用率与R1期NPQ呈正相关关系,而与R1期qL呈负相关关系;R1期PSⅡ实际光化学效率(Φ_PSⅡ)与磷高效基因型呈负相关,而与磷低效基因型呈正相关,这表明R1期Φ_PSⅡ可以作为鉴定低铁条件下不同磷效率大豆基因型的一个重要指标。利用因子得分综合评价发现,磷高效基因型表现为随着磷水平的上升而先降后升,而磷低效基因型则为先升后降,但两者拐点均出现在P∶Fe为30∶30处理下,这表明在低铁条件下P∶Fe为30∶30可以作为鉴定不同磷效率基因型的一个临界值。因而,在低铁地区种植磷高效大豆基因型时,磷肥的施用量至少要大于1∶1 (P∶Fe);而种植磷低效基因型时,磷肥的施用量不宜超过1∶1 (P∶Fe)。

关键词: 叶绿素荧光特性, 磷利用率, 铁利用率, 磷:铁

Abstract: We examined the effects of phosphorus (P) levels on photosynthetic and P/Fe traits of soybean under the stress of low Fe and their genotypic differences, to provide a theoretical basis for rational application of P and Fe fertilizer. Six P-efficient and six P-inefficient soybean varieties screened in the early stage were used as experimental materials. Four treatments of P:Fe ratio were set, including 0:30, 30:30, 150:30 and 300:30 (μmol·L^-1). We measured chlorophyll fluorescence traits and P-Fe utilization efficiency in soybean. A stepwise regression equation was established with seed weight per plant. Pathway analysis was performed, with the response of P-efficient and P-inefficient soybean genotypes to different P:Fe treatments being comprehensively evaluated by factor scores. The results showed significant main and interactive effects of genotype and P:Fe on the relative electron transfer rate of photosystem Ⅱ (ETR) at beginning of flowering stage (R1), the proportion of the energy absorbed by photosystem Ⅱ dissipated into heat (NPQ) at R1 stage, and proportion of energy absorbed by photosystem Ⅱ devoted to the photochemical reaction (qL) at R1 stage. Results of canonical correlation analysis showed a negative correlation between P utilization efficiency of seed at full maturity stage (R8) and photosynthetic rate at R1 stage of P-efficient genotypes. Seed Fe utilization efficiency of P-inefficient genotypes at R8 stage was positively correlated with NPQ at R1 stage, but negatively correlated with qL at R1 stage. The actual photochemical efficiency of PSⅡ (Φ_PSⅡ) at R1 stage was negatively correlated with P-efficient genotypes, but positively correlated with P-inefficient genotypes, which indicated that Φ_PSⅡ at R1 stage was an important indicator for identifying soybean genotypes with different P efficiency under stress of low Fe. The comprehensive performance of P-efficient soybean genotypes decreased first and then increased with P level, while P-inefficient soybean genotypes increased first and then decreased. The inflection point of both genotypes appeared in P:Fe of 30:30. Thus, P:Fe ratio of 30:30 could be used as a threshold to identify soybean genotypes with different P efficiency under stress of low Fe. In conclusion, P fertilizer application should be equal to or greater than 1:1 (P:Fe) when planting P-efficient soybean genotypes in low Fe area, while P fertilizer application should not exceed 1:1 (P:Fe) when planting P-inefficient soybean genotypes.

Key words: chlorophyll fluorescence trait, P utilization efficiency, Fe utilization efficiency, P:Fe

赵婧, 孟凡钢, 于德彬, 张鸣浩, 饶德民, 丛博韬, 闫晓艳, 张伟, 邱强. 铁胁迫下大豆光合和磷/铁性状对磷素的响应[J]. 应用生态学报, 2021, 32(5): 1768-1776.

ZHAO Jing, MENG Fan-gang, YU De-bin, ZHANG Ming-hao, RAO De-min, CONG Bo-tao, YAN Xiao-yan, ZHANG Wei, QIU Qiang. Responses of photosynthesis and P/Fe traits to P application in soybean under stress of low Fe.[J]. Chinese Journal of Applied Ecology, 2021, 32(5): 1768-1776.

参考文献

[1] Lin Y, Chen G, Hu H, et al. Phenotypic and genetic variation in phosphorus-deficiency-tolerance traits in Chinese wheat landraces. BMC Plant Biology, 2020, 20: 330
[2] Kaur G, Shukla V, Meena V, et al. Plant biology: Underpinning wheat physiological and molecular responses to co-occurring iron and phosphate deficiency stress. News of Science, 2020, doi: 10.1101/2020.05.26.117101
[3] 赵婧, 邱强, 张鸣浩, 等. 植物体内磷铁平衡与缺铁胁迫的关系研究进展. 作物研究, 2016, 30(3): 343-346 [Zhao J, Qiu Q, Zhang M-H, et al. Research progress on the relationship between P-Fe balance and Fe deficiency stress. Crop Research, 2016, 30(3): 343-346]
[4] Ahmed MF, Kennedy IR, Choudhury ATMA, et al. Phosphorous adsorption in some Australian soils and influence of bacteria on the desorption of phosphorous. Communications in Soil Science and Plant Analysis, 2008, 39: 1269-1294
[5] Sims JT, Pierzynski GM. Chemistry of phosphorus in soils// Tabatabai MA, Sparks DL, eds. Chemical Processes in Soils. Soil Science Society of America Book Series 8. Soil Science Society of America, Madison, WI, USA, 2005: 151-192
[6] Ayenew B, Tadesse AM, Kibret K, et al. Phosphorous status and adsorption characteristics of acid soils from Cheha and Dinsho districts, southern highlands of Ethiopia. Environmental Systems Research, 2018, 7: 17
[7] Lucena C, Romera FJ, García MJ, et al. Ethylene participates in the regulation of Fe deficiency responses in strategy. I. Plants and in rice. Frontiers in Plant Science, 2015, 6: 1056, doi: 10.3389/fpls.2015.01056.eCollection 2015
[8] Song L, Dong L. Ethylene and plant responses to phosphate deficiency. Frontiers in Plant Science, 2015, 6: 796, doi: 10.3389/fpls.2015.00796. eCollection 2015
[9] Neumann G. The role of ethylene in plant adaptations for phosphate acquisition in soils: A review. Frontiers in Plant Science, 2016, 6: 1224, doi: 10.3389/fpls.2015.01224.eCollection 2015
[10] Hirsch J, Marin E, Floriani M, et al. Phosphate deficiency promotes modification of iron distribution in Arabidopsis plants. Biochimie, 2006, 88: 1767-1771
[11] Misson J, Raghothama KG, Jain A, et al. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proceedings of the National Acade-my of Sciences of the United States of America, 2005, 102: 11934-11939
[12] Ward JT, Lahner B, Yakubova E, et al. The effect of iron on the primary root elongation of Arabidopsis during phosphate deficiency. Plant Physiology, 2008, 147: 1181-1191
[13] 何春娥, 刘学军, 张福锁. 植物根表铁膜的形成及其营养与生态环境效应. 应用生态学报, 2004, 15(6): 1069-1073 [He C-E, Liu X-J, Zhang F-S. Formation of iron plaque on root surface and its effect on plant nutrition and ecological environment. Chinese Journal of Applied Ecology, 2004, 15(6): 1069-1073]
[14] 夏海勇, 薛艳芳, 孟维伟, 等. 间套作体系作物-土壤铁和锌营养研究进展. 应用生态学报, 2015, 26(4): 1263-1270 [Xia H-Y, Xue Y-F, Meng W-W, et al. Research advances in iron and zinc transfer from soil to plant in intercropping systems. Chinese Journal of Applied Ecology, 2015, 26(4): 1263-1270]
[15] 陈远学, 李汉邯, 周涛, 等. 施磷对间套作玉米叶面积指数、干物质积累分配及磷肥利用效率的影响. 应用生态学报, 2013, 24(10): 2799-2806 [Chen Y-X, Li H-H, Zhou T, et al. Effects of phosphorus fertilization on leaf area index,biomass accumulation and allocation,and phosphorus use efficiency of intercropped maize. Chinese Journal of Applied Ecology, 2013, 24(10): 2799-2806]
[16] 赵伟, 宋春, 周攀, 等. 施磷量与施磷深度对玉米-大豆套作系统磷素利用率及磷流失风险的影响. 应用生态学报, 2018, 29(4): 1205-1214 [Zhao W, Song C, Zhou P, et al. Effects of phosphorus application rates and depths on P utilization and loss risk in a maize-soybean intercropping system. Chinese Journal of Applied Ecology, 2018, 29(4): 1205-1214]
[17] Kim SA, Guerinot ML. Mining iron: Iron uptake and transport in plants. FEBS Letters, 2007, 581: 2273-2280
[18] Raghothama KG. Phosphorus acquisition. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 50: 665-693
[19] Qiu W, Dai J, Wang N, et al. Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean. Plant Physiology & Biochemistry, 2017, 112: 1-8
[20] 张雪洁, 谭晓风, 袁军, 等. 低磷胁迫对油茶叶绿素荧光参数的影响. 经济林研究, 2012, 30(2): 48-51 [Zhang X-J, Tan X-F, Yuan J, et al. Effects of low phosphorus stress on chlorophyll fluorescence parameters in Camellia oleifera. Non-wood Forest Research, 2012, 30(2): 48-51]
[21] Aro EM, Virgin I, Andersson B. Photoinhibition of photosystem Ⅱ. Inactivation, protein damage and turnover. Biochimica et Biophysica Acta-Bioenergetics, 1993, 1143: 113-134
[22] 陈建明, 俞晓平, 程家安. 叶绿素荧光动力学及其在植物抗逆生理研究中的应用. 浙江农业学报, 2006, 18(1): 51-55 [Chen J-M, Yu X-P, Cheng J-A. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agriculturae Zhejiangensis, 2006, 18(1): 51-55]
[23] 成敏敏, 陈柯伊, 朱雪玉, 等. 花叶矢竹复绿期光合特性及叶绿体结构. 林业科学, 2018, 54(4): 1-10 [Cheng M-M, Chen K-Y, Zhu X-Y, et al. Photosynthetic characteristics and chloroplast ultrastructure of Pseudosasa japonica f. akebonosuji during green-revertible albino stage. Scientia Silvae Sinicae, 2018, 54(4): 1-10]
[24] 杨伟波, 付登强, 李艳, 等. 不同光强下义安油茶幼苗生长和叶绿素荧光特性分析. 热带作物学报, 2012, 33(4): 651-654 [Yang W-B, Fu D-Q, Li Y, et al. Growth and chlorophyll fluorescence of Camellia oleifera var. ‘nhge an' seedlings under different light intensity. Chinese Journal of Tropical Crops, 2012, 33(4): 651-654]
[25] Jeong K, Julia CC, Waters DL, et al. Remobilisation of phosphorus fractions in rice flag leaves during grain fil-ling: Implications for photosynthesis and grain yields. PLoS One, 2017, 12(11): e0187521
[26] Alscher RG, Erturk N, Heath LS. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, 2002, 53: 1331-1341
[27] Liu K, Ma B, Luan L, et al. Nitrogen, phosphorus, and potassium nutrient effects on grain filling and yield of high-yielding summer corn. Journal of Plant Nutrition, 2011, 34: 1516-1531
[28] Sanchez-Rodriguez AR, del Campillo MC, Torrent J. The severity of iron chlorosis in sensitive plants is related to soil phosphorus levels. Journal of the Science of Food and Agriculture, 2014, 94: 2766-2773
[29] Sanchez-Rodriguez AR, del Campillo MC, Torrent J. Phosphate aggravates iron chlorosis in sensitive plants grown on model calcium carbonate-iron oxide systems. Plant and Soil, 2013, 373: 31-42
[30] 黄洁雪, 闫明科, 薛彩纹, 等. 外界铁浓度调控缺磷植物铁吸收相关基因的表达量. 土壤, 2018, 50(5): 866-873 [Huang J-X, Yan M-K, Xue C-W, et al. Phosphate deficiency induced down-regulation of iron acquisition genes is dependent on ambient iron concentrations. Soils, 2018, 50(5): 866-873]
[31] Hirsch J, Marin E, Floriani M, et al. Phosphate deficiency promotes modification of iron distribution in Arabidopsis plants. Biochimie, 2006, 88: 1767-1771
[32] Maharajan T, Ceasar SA, Krishna TPA, et al. Phosphate supply influenced the growth, yield and expression of PHT1 family phosphate transporters in seven millets. Planta, 2019, 250: 1433-1448
[33] Hur YJ, Lee HG, Jeon EJ, et al. A phosphate starvation-induced acid phosphatase from Oryza sativa: Phosphate regulation and transgenic expression. Biotechnology Letters, 2007, 29: 829-835
[34] Ganie AH, Ahmad A, Pandey R, et al. Metabolite profiling of low-P tolerant and low-P sensitive maize genotypes under phosphorus starvation and restoration conditions. PLoS One, 2015, 10(6): e0129520
[35] Pandey R, Dubey KK, Ahmad A, et al. Elevated CO₂ improves growth and phosphorus utilization efficiency in cereal species under sub-optimal phosphorus supply. Journal of Plant Nutrition, 2015, 38: 1196-1217

铁胁迫下大豆光合和磷/铁性状对磷素的响应

Responses of photosynthesis and P/Fe traits to P application in soybean under stress of low Fe.

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