应用生态学报 ›› 2022, Vol. 33 ›› Issue (11): 3146-3158.doi: 10.13287/j.1001-9332.202211.010
唐加菜, 魏成梅, 赵婧, 严乃胜, 董文霞*
收稿日期:
2022-01-04
修回日期:
2022-08-23
出版日期:
2022-11-15
发布日期:
2023-05-15
通讯作者:
*E-mail: dongwenxia@163.com
作者简介:
唐加菜, 女, 1997年生, 硕士研究生。主要从事昆虫化学生态学研究。E-mail: tjc14769635501@163.com
基金资助:
TANG Jia-cai, WEI Cheng-mei, ZHAO Jing, YAN Nai-sheng, DONG Wen-xia*
Received:
2022-01-04
Revised:
2022-08-23
Online:
2022-11-15
Published:
2023-05-15
摘要: 单感器记录技术是一种昆虫细胞外电生理技术,可以测量昆虫单个感受器对刺激物的电生理反应。该技术有助于探明昆虫嗅觉和味觉感受器对不同信息化合物的电生理响应机制,将单感器记录技术与其他技术相结合,不仅可以阐明昆虫嗅觉反应的分子机制,还可以研制昆虫行为调节剂、检测挥发性有机化合物的生物传感器。本文介绍了单感器记录仪的结构和昆虫单感器记录的原理,并对单感器记录技术在昆虫学研究方面的应用进行了综述,以期为探明昆虫感受化学信息物质的机理和应用提供依据。
唐加菜, 魏成梅, 赵婧, 严乃胜, 董文霞. 昆虫单个感觉器记录技术研究进展[J]. 应用生态学报, 2022, 33(11): 3146-3158.
TANG Jia-cai, WEI Cheng-mei, ZHAO Jing, YAN Nai-sheng, DONG Wen-xia. Research progress on insect single sensillum recording.[J]. Chinese Journal of Applied Ecology, 2022, 33(11): 3146-3158.
[1] 尹海辰, 李文静, 许敏, 等. 昆虫嗅觉视觉信号识别及相关引诱技术研究进展. 湖北植保, 2020(5): 56-64 [2] Fan J, Francis F, Liu Y, et al. Review an overview of odorant-binding protein functions in insect peripheral olfactory reception. Genetics & Molecular Research, 2011, 10: 3056-3069 [3] Hua JF, Zhang S, Cui JJ, et al. Identification and bin-ding characterization of three odorant binding proteins and one chemosensory protein from Apolygus lucorum (Meyer-Dur). Journal of Chemical Ecology, 2012, 38: 1163-1170 [4] 娄永根, 程家安. 昆虫的化学感觉机理. 生态学杂志, 2001, 20(2): 66-69 [5] 杜永均, 严福顺. 植物挥发性次生物质在植食性昆虫、寄生植物和昆虫天敌关系中的作用机理. 昆虫学报, 1994, 37(2): 233-250 [6] Olsson SB, Hansson BS. Electroantennogram and single sensillum recording in insect antennae. Methods in Molecular Biology, 2013, 1068: 157-177 [7] 黄翠虹, 李静静, 周琳, 等. 昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术. 应用昆虫学报, 2014, 51(2): 579-585 [8] Boeckh J. Electrophysiological studies on individual olfactory receptors on the antennae of the gravedigger (Necrophorus: Coleoptera). Journal of Comparative Physiology A, 1962, 46: 212-248 [9] Kaissling KE. Sensory transduction in insect olfactory receptors// Jaenicke L, ed. Biochemistry of Sensory Functions. New York: Springer, 1974: 243-273 [10] 严福顺. 鳞翅目昆虫的味觉感受器及其电生理研究方法. 昆虫知识, 1995, 32(3): 169-172 [11] Hansson BS. Olfaction in Lepidoptera. Cellular and Molecular Life Sciences, 1995, 51: 18742 [12] 汤清波, 马英, 黄玲巧, 等. 昆虫味觉感受机制研究进展. 昆虫学报, 2011, 54(12): 1433-1444 [13] Schoonhoven LM, van Loon JJA, Dicke M. Insect-Plant Biology. 2nd Ed. Oxford: Oxford University Press, 2005: 183-189 [14] 王艳. 蔗糖和黑芥子苷对铃夜蛾属两近缘种昆虫取食选择行为和味觉感受的影响. 硕士论文. 郑州: 河南农业大学, 2018 [15] Liu F, Liu N. Using single sensillum recording to detect olfactory neuron responses of bed bugs to semiochemicals. Journal of Visualized Experiments, 2016,107: e53337 [16] Dawson GW, Griffiths DC, Pickett JA, et al. Plant-derived synergists of alarm pheromone from turnip aphid, Lipaphis erysimi (Homoptera: Aphididae). Journal of Chemical Ecology, 1987, 13: 1663-1671 [17] Ng R, Lin HH, Wang JW, et al. Electrophysiological recording from Drosophila trichoid sensilla in response to odorants of low volatility. Journal of Visualized Experiments, 2017, 125: e56147 [18] 王婵. 黏虫Mythimna separata性信息素受体及神经的功能研究. 硕士论文. 北京: 中国农业科学院, 2019 [19] Chang HT, Guo MB, Wang B, et al. Sensillar expression and responses of olfactory receptors reveal different peripheral coding in two Helicoverpa species using the same pheromone components. Scientific Reports, 2016, 6: 18742 [20] Jiang NJ, Tang R, Guo H, et al. Olfactory coding of intra- and interspecific pheromonal messages by the male Mythimna separata in north China. Insect Biochemistry and Molecular Biology, 2020, 125: 103439 [21] Jiang NJ, Mo BT, Guo H, et al. Revisiting the sex pheromone of the fall armyworm Spodoptera frugiperda, a new invasive pest in south China. Insect Science, 2022, 29: 865-878 [22] Guo H, Gong XL, Li GC, et al. Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda. Pest Management Science, 2022, 78: 2052-2064 [23] 吴才宏, 刘启渊. 蓖麻蚕雄蛾触角的嗅觉感受细胞对性信息素各组分的反应. 昆虫学报, 1996, 39(2): 121-125 [24] Xu P, Garczynski SF, Atungulu E, et al. Moth sex pheromone receptors and deceitful parapheromones. PLoS One, 2012, 7(7): e41653 [25] Wu H, Hou C, Huang LQ, et al. Peripheral coding of sex pheromone blends with reverse ratios in two Helico-verpa species. PLoS One, 2013, 8(7): e70078 [26] Rouyar A, Deisig N, Dupuy F, et al. Unexpected plant odor responses in a moth pheromone system. Frontiers in Physiology, 2015, 6: 148 [27] Xu M, Guo H, Hou C, et al. Olfactory perception and behavioral effects of sex pheromone gland components in Helicoverpa armigera and Helicoverpa assulta. Scientific Reports, 2016, 6: 22998 [28] Zielonka M, Gehrke P, Badeke E, et al. Larval sensilla of the moth Heliothis virescens respond to sex pheromone components. Insect Molecular Biology, 2016, 25: 666-678 [29] Cui WC, Wang B, Guo MB, et al. A receptor-neuron correlate for the detection of attractive plant volatiles in Helicoverpa assulta (Lepidoptera: Noctuidae). Insect Biochemistry and Molecular Biology, 2018, 97: 31-39 [30] Liu W, Jiang XC, Cao S, et al. Functional studies of sex pheromone receptors in Asian corn borer Ostrinia furnacalis. Frontiers in Physiology, 2018, 9: 591 [31] Wanner KW, Moore K, Wei J, et al. Pheromone odo-rant receptor responses reveal the presence of a cryptic, redundant sex pheromone component in the European corn borer, Ostrinia nubilalis. Journal of Chemical Eco-logy, 2020, 46: 567-580 [32] 马百伟, 刘晓岚, 常亚军, 等. 利用单感器记录与神经元示踪结合对棉铃虫主要性信息素感器内神经元投射的鉴定. 昆虫学报, 2020, 63(4): 413-420 [33] Tang R, Jiang NJ, Ning C, et al. The olfactory reception of acetic acid and ionotropic receptors in the Oriental armyworm, Mythimna separata walker. Insect Biochemistry and Molecular Biology, 2020, 118: 103312 [34] Sun JG, Huang LQ, Wang CZ. Electrophysiological and behavioral responses of Helicoverpa assulta (Lepidoptera: Noctuidae) to tobacco volatiles. Arthropod-Plant Interactions, 2012, 6: 375-384 [35] 李冠楠. 烟夜蛾对烟草挥发物嗅觉识别分子机制的研究. 硕士论文. 郑州: 河南农业大学, 2018 [36] Wang C, Li G, Miao C, et al. Nonanal modulates oviposition preference in female Helicoverpa assulta (Lepidoptera: Noctuidae) via the activation of peripheral neurons. Pest Management Science, 2020, 76: 3159-3167 [37] Blanka P. Olfactory responses recorded from sensilla coeloconica of the silkmoth Bombyx mori. Physiological Entomology, 1997, 22: 239-248 [38] Anfora G, Vitagliano S, Larsson MC, et al. Disruption of Phthorimaea operculella (Lepidoptera: Gelechiidae) oviposition by the application of host plant volatiles. Pest Management Science, 2014, 70: 628-635 [39] Wee SL, Oh HW, Park KC. Antennal sensillum morphology and electrophysiological responses of olfactory receptor neurons in trichoid sensilla of the diamondback moth (Lepidoptera: Plutellidae). Florida Entomologist, 2016, 99: 146-158 [40] Park KC, Lee JA, Suckling DM. Antennal olfactory sensory neurones responsive to host and nonhost plant volatiles in gorse pod moth Cydia succedana. Physiological Entomology, 2017, 43: 86-99 [41] 张钟宁, 涂美华, 杜永均, 等. 桃蚜对[反]-β-法尼烯的行为及电生理反应. 昆虫学报, 1997, 40(1): 40-44 [42] Dawson GW, Griffiths DC, Pickett JA, et al. Plant-derived synergists of alarm pheromone from turnip aphid, Lipaphis erysimi (Homoptera: Aphididae). Journal of Chemical Ecology, 1987, 13: 1663-1671 [43] Zhang RB, Wang B, Grossi G, et al. Molecular basis of alarm pheromone detection in aphids. Current Biology, 2017, 27: 55-61 [44] 王璐. 基于SSR和EAG的麦长管蚜及其寄生性天敌的嗅觉反应研究. 硕士论文. 泰安: 山东农业大学, 2019 [45] Bromley AK, Anderson M. An electrophysiological study of olfaction in the aphid Nasonovia ribisnigri. Entomologia Experimentalis et Applicata, 1982, 32: 101-110 [46] 王振华, 赵晖, 李金甫, 等. 植物源挥发物对昆虫信息素的增效作用及其增效机制. 应用生态学报, 2008, 19(11): 2533-2537 [47] Liu F, Liu N. Human odorant reception in the common bed bug, Cimex lectularius. Scientific Reports, 2015, 5: 15558 [48] Keesey IW, Knaden M, Hansson BS. Olfactory specialization in Drosophila suzukii supports an ecological shift in host preference from rotten to fresh fruit. Journal of Chemical Ecology, 2015, 41: 121-128 [49] Dekker T, Irene I, Siju KP, et al. Olfactory shifts para-llel superspecialism for toxic fruit in Drosophila melanogaster sibling, D. sechellia. Current Biology, 2006, 16: 101-109 [50] Gonzalez F, Witzgall P, Walker WB. Protocol for hetero-logous expression of insect odourant receptors in Drosophila. Frontiers in Ecology and Evolution, 2016, 4: 24 [51] 游银伟. 果蝇嗅觉受体Or82a及飞蝗嗅觉受体LmigOR3的功能研究. 博士论文. 北京: 中国农业大学, 2017 [52] Meijerink J, Braks M, van Loon JJA. Olfactory receptors on the antennae of the malaria mosquito Anopheles gambiae are sensitive to ammonia and other sweat-borne components. Journal of Insect Physiology, 2001, 47: 455-464 [53] Ditzen M, Pellegrino M, Vosshall LB. Insect odorant receptors are molecular targets of the insect repellent DEET. Science, 2008, 319: 1838-1842 [54] Ghaninia M, Ignell R, Hansson BS. Functional classification and central nervous projections of olfactory receptor neurons housed in antennal trichoid sensilla of female yellow fever mosquitoes, Aedes aegypti. European Journal of Neuroscience, 2007, 26: 1611-1623 [55] Chen Z, Liu F, Liu NN. Neuronal responses of antennal olfactory sensilla to insect chemical repellents in the yellow fever mosquito, Aedes aegypti. Journal of Chemical Ecology, 2018, 44: 1120-1126 [56] Sun H, Liu F, Baker AP, et al. Neuronal odor coding in the larval sensory cone of Anopheles coluzzii: Complex responses from a simple system. Cell Reports, 2021, 36: 109555 [57] Syed Z, Leal WS. Mosquitoes smell and avoid the insect repellent DEET. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105: 13598-13603 [58] Ochieng SA, Hansson BS. Responses of factory receptor neurones to behaviourally important odours in gregarious and solitary desert locust, Schistocerca gregaria. Physiological Entomology, 1999, 24: 28-36 [59] Cui XJ, Wu CH, Zhang L. Electrophysiological response patterns of 16 olfactory neurons from the trichoid sensilla to odorant from fecal volatiles in the locust, Locusta migratoria manilensis. Insect Biochemistry & Physiology, 2011, 77: 45-57 [60] Li HW, You YW, Zhang L. Single sensillum recordings for locust palp sensilla basiconica. Journal of Visualized Experiments, 2018, 136: e57863 [61] 李红卫. 飞蝗口器嗅觉神经元编码特征及一种气味分子受体功能的研究. 博士论文. 北京: 中国农业大学, 2019 [62] Wei JR, Zhou Q, Hall L, et al. Olfactory sensory neurons of the Asian longhorned beetle, Anoplophora glabripennis, specifically responsive to its two aggregation-sex pheromone components. Journal of Chemical Eco-logy, 2018, 44: 637-649 [63] Hall LP, Graves F, Myrick A, et al. Labial and maxillary palp recordings of the Asian longhorned beetle, Anoplophora glabripennis, reveal olfactory and hygroreceptive capabilities. Journal of Insect Physiology, 2019, 117: 103905 [64] Lohonyai Z, Vuts J, Kárpáti Z, et al. Benzaldehyde: An alfalfa-related compound for the spring attraction of the pest weevil Sitona humeralis (Coleoptera: Curculionidae). Pest Management Science, 2019, 75: 3153-3159 [65] Park KC, McNeill MR, Suckling DM, et al. Olfactory receptor neurons for plant volatiles and pheromone compounds in the lucerne weevil, Sitona discoideus. Journal of Chemical Ecology, 2020, 46: 250-263 [66] Larsson MC, Leal WS, Hansson BS. Olfactory receptor neurons specific to chiral sex pheromone components in male and female Anomala cuprea beetles (Coleoptera: Scarabaeidae). Journal of Comparative Physiology A, 1999, 184: 353-359 [67] Larsson MC, Leal WS, Hansson BS. Olfactory receptor neurons detecting plant odours and male volatiles in Anomala cuprea beetles (Coleoptera: Scarabaeidae). Journal of Insect Physiology, 2001, 47: 1065-1076 [68] Ochieng SA, Park KC, Zhu JW, et al. Functional morphology of antennal chemoreceptors of the parasitoid Microplitis croceipes (Hymenoptera: Braconidae). Arth-ropod Structure & Development, 2000, 29: 231-240 [69] Ezaki K, Yamashita T, Carle T, et al. Aldehyde-specific responses of olfactory sensory neurons in the praying mantis. Scientific Reports, 2021, 11: 1856 [70] 魏宇. 两种盲蝽同源气味结合蛋白基因OBP11的克隆和功能研究. 硕士论文. 北京: 中国农业科学院, 2016 [71] Schoonhoven LM, vanLoon JJA. An inventory of taste in caterpillars: Each species its own key. Acta Zoologica Academiae Scientiarum Hungaricae, 2002, 48: 215-263 [72] 侯文华, 孙龙龙, 马英, 等. 草地贪夜蛾幼虫对四种刺激物质的味觉感受和取食选择. 昆虫学报, 2020, 63(5): 545-557 [73] Yang S, Cao D, Wang G, et al. Identification of genes involved in chemoreception in Plutella xylostella by antennal transcriptome analysis. Scientific Reports, 2017, 7: 11941 [74] Yang K, Gong XL, Li GC, et al. A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae). eLife Sciences, 2020, 9: e64114 [75] Yang J, Guo H, Jiang NJ, et al. Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae. PLoS Genetics, 2021, 17: e1009527 [76] 汤德良, 王琛柱, 罗林儿, 等. 棉铃虫和烟青虫幼虫下颚栓锥感器对某些化合物反应特性的比较. 中国科学: 生命科学, 2000, 30(5): 511-516 [77] 曹欢. 棉铃虫Helicoverpa armigera (Hübner)和烟青虫H. assulta(Guenée)幼虫味觉感受的比较研究. 硕士论文. 郑州: 河南农业大学, 2013 [78] 贾岩岩. 雌性棉铃虫触角刺形感器和喙管栓锥感器的味觉感受特性. 硕士论文. 合肥: 安徽大学, 2016 [79] 张佳佳, 侯文华, 孙龙龙, 等. Helicoverpa两近缘种昆虫对果糖、葡萄糖及氨基酸的味觉电生理反应. 华中昆虫研究, 2020, 16(1): 358 [80] 严福顺, Schoonhoven LM. 大菜粉蝶幼虫外颚叶味觉感器对蓼二醛的电生理反应. 昆虫学报, 1993, 36(1): 1-7 [81] Inoue TA, Asaoka K, Seta K, et al. Sugar receptor response of the food-canal taste sensilla in a nectar-feeding swallowtail butterfly, Papilio xuthus. Naturwissenschaften, 2009, 96: 355-363 [82] 张辉洁. 家蚕食物选择行为的生理学比较及味觉受体基因BmGr8的功能研究. 博士论文. 重庆: 西南大学, 2011 [83] Martin TL, Shields VDD. Detection of alkaloids and carbohydrates by taste receptor cells of the galea of gypsy moth larvae, Lymantria dispar (L.). Arthropod-Plant Interactions, 2012, 6: 519-529 [84] Miriyala A, Sébastien Kessler F, et al. Burst firing in bee gustatory neurons prevents adaptation. Current Bio-logy, 2018, 28: 1585-1594 [85] Thorne N, Chromey C, Bray S, et al. Taste perception and coding in Drosophila. Current Biology, 2004, 14: 1065-1079 [86] Dahanukar A, Lei YT, Kwon JY, et al. Two Gr genes underlie sugar reception in Drosophila. Neuron, 2007, 56: 503-516 [87] Ling F, Dahanukar A, Weiss LA, et al. The molecular and cellular basis of taste coding in the legs of Droso-phila. Journal of Neuroscience, 2014, 34: 7148-7164 [88] Zhang YF, van Loon JJA, Wang CZ. Tarsal taste neuron activity and proboscis extension reflex in response to sugars and amino acids in Helicoverpa armigera (Hübner). Journal of Experimental Biology, 2010, 213: 2889-2895 [89] Zhang YF, Huang LQ, Ge F, et al. Tarsal taste neurons of Helicoverpa assulta (Guenée) respond to sugars and amino acids, suggesting a role in feeding and oviposition. Journal of Insect Physiology, 2011, 57: 1332-1340 [90] Seada MA, Ignell R, Assiuty AN, et al. Functional characterization of the gustatory sensilla of tarsi of the female polyphagous moth Spodoptera littoralis. Frontiers in Physiology, 2018, 9: 1606 [91] De Brito Sanchez MG, Lorenzo E, Su S, et al. The tarsal taste of honey bees: Behavioral and electrophysiolo-gical analyses. Frontiers in Behavioral Neuroscience, 2014, 8: 25 [92] Merivee E, Must A, Milius M, et al. Electrophysiological identification of the sugar cell in antennal taste sensilla of the predatory ground beetle Pterostichus aethiops. Journal of Insect Physiology, 2007, 53: 377-384 [93] Pentzold S, Marion-Poll F, Grabe V, et al. Autofluorescence-based identification and functional validation of antennal gustatory sensilla in a specialist leaf beetle. Frontiers in Physiology, 2019, 10: 343 [94] Iacovone A, Salerno G, French AS, et al. Antennal gustatory perception and behavioural responses in Trissolcus brochymenae females. Journal of Insect Physiology, 2015, 78: 15-25 [95] Jiang XJ, Ning C, Guo H, et al. A gustatory receptor tuned to D-fructose in antennal sensilla chaetica of Helicoverpa armigera. Insect Biochemistry and Molecular Biology, 2015, 60: 39-46 [96] Popescu A, Couton L, Almaas T. Function and central projections of gustatory receptor neurons on the antenna of the noctuid moth Spodoptera littoralis. Journal of Comparative Physiology A, 2013, 199: 403-416 [97] Jrgensen K, Almaas JT, Marion-Poll F, et al. Electrophysiological characterization of responses from gustatory receptor neurons of sensilla chaetica in the moth Heliothis virescens. Chemical Senses, 2007, 32: 863-879 [98] Sethi S, Lin HH, Shepherd AK, et al. Social context enhances hormonal modulation of pheromone detection in Drosophila. Current Biology, 2019, 29: 3887-3898 [99] Merivee E, Must A, Nurme K, et al. Neural code for ambient heat detection in elaterid beetles. Frontiers in Behavioral Neuroscience, 2020, 14: 1 |
[1] | 吴远秀, 刘婧桐, 丁聪, 张炳川, 梁潇洒, 宁宇, 殷江霞, 吕晓涛. 氮素输入和刈割对草甸草原植食性昆虫多度和物种丰富度的影响 [J]. 应用生态学报, 2023, 34(7): 1975-1980. |
[2] | 邱可睿, 李景功, 刘文, 王小平. 昆虫敏感波长测定的研究进展 [J]. 应用生态学报, 2023, 34(5): 1430-1440. |
[3] | 王花, 王慧, 邵晓莉, 叶忠铭. 田边伴生植物种植提升作物传粉服务功能的研究现状与展望 [J]. 应用生态学报, 2023, 34(10): 2854-2860. |
[4] | 覃海蓉, 郭文锋, 王伟, 阳莎, 李晓琼. 莲草直胸跳甲取食对空心莲子草和莲子草克隆整合的影响 [J]. 应用生态学报, 2022, 33(6): 1661-1668. |
[5] | 吴磊, 孙奇, 赵骥民, 王德利, 张彦文. 花部重金属积累对植物-传粉昆虫互惠关系影响的研究进展 [J]. 应用生态学报, 2022, 33(5): 1429-1434. |
[6] | 周颖, 刘杰, 闫晓慧, 胡世俊. 模拟昆虫取食对牛膝菊防御特征的影响 [J]. 应用生态学报, 2022, 33(3): 808-812. |
[7] | 张月白, 娄永根. 植物与植食性昆虫化学互作研究进展 [J]. 应用生态学报, 2020, 31(7): 2151-2160. |
[8] | 陈瑾, 王建武, 舒迎花. 重金属污染影响植食性昆虫的研究进展 [J]. 应用生态学报, 2020, 31(5): 1773-1782. |
[9] | 郭祖国, 王梦馨, 崔林, 韩宝瑜. 昆虫趋色性及诱虫色板的研究和应用进展 [J]. 应用生态学报, 2019, 30(10): 3615-3626. |
[10] | 王美娜, 丁圣彦, 卢训令, 崔洋. 豫西山地传粉昆虫物种多样性对地貌和季节的响应 [J]. 应用生态学报, 2017, 28(9): 2879-2887. |
[11] | 吴龙飞, 姜文虎, 袁胜亮, 郭萌萌, 刘军侠. 塞罕坝自然保护区樟子松不同林分类型对昆虫群落多样性的影响 [J]. 应用生态学报, 2017, 28(1): 308-314. |
[12] | 王润, 丁圣彦, 卢训令, 宋博. 黄河中下游农业景观异质性对传粉昆虫多样性的多尺度效应——以巩义市为例 [J]. 应用生态学报, 2016, 27(7): 2145-2153. |
[13] | 雒珺瑜, 张帅, 朱香镇, 吕丽敏, 王春义, 李春花, 张利娟, 王丽, 崔金杰. 转GAFP基因棉花生态适合度及其对棉田昆虫群落的影响 [J]. 应用生态学报, 2016, 27(11): 3675-3681. |
[14] | 陈发军1,2,杨青青1,龙黎1,胡红梅1,段彬1,陈文年1,2*. 城市园林生境冬季中华蜜蜂的活动规律和采集行为 [J]. 应用生态学报, 2016, 27(1): 275-281. |
[15] | 刘哲强1,马玲1**,焦玥1,张静2,曹传旺1,顾伟1,满子源1,张曼胤3. 小兴安岭森林恢复期典型人工林与原始林昆虫群落结构动态 [J]. 应用生态学报, 2015, 26(2): 555-562. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||