番茄青枯病生物防治的研究进展

doi:10.13287/j.1001-9332.202309.028

应用生态学报 ›› 2023, Vol. 34 ›› Issue (9): 2585-2592.doi: 10.13287/j.1001-9332.202309.028

• • 上一篇

番茄青枯病生物防治的研究进展

吴思炫^1,2,3, 高复云^1,2,3, 张锐澎^1,2,3, 苏浩^1,2, 姚槐应⁴, 范雪莲⁵, 李雅颖^1,2,3*

¹中国科学院城市环境研究所, 城市环境与健康重点实验室, 福建厦门 361021;
²宁波(北仑)中科海西产业技术创新中心, 浙江省城市环境过程与污染控制重点实验室, 浙江宁波 315800;
³中国科学院大学, 北京 100049;
⁴武汉工程大学, 武汉 430074;
⁵宁波市农业技术推广总站, 浙江宁波 315800

收稿日期:2023-03-30 修回日期:2023-07-11 出版日期:2023-09-15 发布日期:2024-03-16
通讯作者: *E-mail: yyli@iue.ac.cn
作者简介:吴思炫, 男, 1999年生, 硕士研究生。主要从事茄科连作障碍研究。E-mail: wsx13322871061@163.com
基金资助:
宁波市科技创新2025重大专项(2021Z047)

Research progress in biological control of tomato bacterial wilt

WU Sixuan^1,2,3, GAO Fuyun^1,2,3, ZHANG Ruipeng^1,2,3, SU Hao^1,2, YAO Huaiying⁴, FAN Xuelian⁵, LI Yaying^1,2,3*

¹Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, Fujian, China;
²Zhejiang Key Laboratory of Urban Environmental Process and Pollution Control, Ningbo (Beilun) Zhongke Haixi Industrial Technology Innovation Center, Ningbo 315800, Zhejiang, China;
³University of Chinese Academy of Sciences, Beijing 100049, China;
⁴Wuhan Institute of Technology, Wuhan 430074, China;
⁵Ningbo Agricultural Technology Promotion Station, Ningbo 315800, Zhejiang, China

Received:2023-03-30 Revised:2023-07-11 Online:2023-09-15 Published:2024-03-16

摘要/Abstract

摘要： 番茄在中国设施蔬菜产量中高居首位,是我国重要的蔬菜作物。青枯病是危害番茄产业发展的重要病害,由青枯雷尔氏菌侵染引起,其病原菌能够在土层深处长时间存活并进行转移,化学防治效果有限,严重影响番茄产量及品质。本文介绍了番茄青枯病的特征及病原菌种类,从植物源杀菌剂、农用抗生素、生防菌、噬菌体等方面系统综述了生物防治番茄青枯病的研究进展,重点介绍了这些生物防治措施的原理、应用现状,同时,对相应方法存在的局限性提出了改进的措施,认为开发出以微生态调控为基础的环保、高效生物防治新体系将是未来番茄青枯病生物防治的发展方向。

关键词: 雷尔氏菌, 生物防治, 土传病害

Abstract: Bacterial wilt caused by the infection of Ralstonia solanacearum, is one of the most harmful diseases to tomatoes, one of the most important greenhouse vegetables in China. R. solanacearum can survive and remain active in the deep soil for a long time, and the chemical control of tomato bacterial wilt is consequently limited. In this study, we introduced the characteristics of tomato bacterial wilt disease and the types of R. solanacearum, and systematically reviewed the research progresses of biological control methods from the aspects of botanical insecticides, agricultural antibiotics, biocontrol bacteria. We emphatically introduced the principle and current status of these methods, discussed the limitations and the improvement strategies, and prospected a new environmental protection and efficient biological control system based on micro-ecological regulation would be the development direction of biological control of tomato bacterial wilt.

Key words: Ralstonia solanacearum, biological control, soil-borne disease

吴思炫, 高复云, 张锐澎, 苏浩, 姚槐应, 范雪莲, 李雅颖. 番茄青枯病生物防治的研究进展[J]. 应用生态学报, 2023, 34(9): 2585-2592.

WU Sixuan, GAO Fuyun, ZHANG Ruipeng, SU Hao, YAO Huaiying, FAN Xuelian, LI Yaying. Research progress in biological control of tomato bacterial wilt[J]. Chinese Journal of Applied Ecology, 2023, 34(9): 2585-2592.

参考文献

[1] 李乐, 田敏娇, 高艳明, 等. 硒肥对基质培番茄生长和矿质元素积累的影响. 浙江农业学报, 2020, 32(2): 253-261
[2] 曾晓娟, 张驰, 何艳清, 等. 基于1980—2019年FAO数据的世界番茄生产状况分析. 湖南农业科学, 2021(11): 104-108
[3] Gao LH, Qu M, Ren HZ, et al. Structure, function, application, and ecological benefit of a single-slope, energy-efficient solar greenhouse in China. HortTechnology, 2010, 20: 626-631
[4] Mohammed AF, Oloyede AR, Odeseye AO. Biological control of bacterial wilt of tomato caused by Ralstonia solanacearum using Pseudomonas species isolated from the rhizosphere of tomato plants. Archives of Phytopatho-logy and Plant Protection, 2020, 53: 1-16
[5] Popoola A, Ganiyu S, Enikuomehin O, et al. Isolation and characterization of Ralstonia solanacearum causing bacterial wilt of tomato in Nigeria. Nigerian Journal of Biotechnology, 2015, 29: 1-10
[6] Paudel S, Dobhal S, Alvarez AM, et al. Taxonomy and phylogenetic research on Ralstonia solanacearum species complex: A complex pathogen with extraordinary economic consequences. Pathogens, 2020, 9: 886
[7] Maji S, Chakrabartty P. Biocontrol of bacterial wilt of tomato caused by “Ralstonia solanacearum” by isolates of plant growth promoting rhizobacteria. Australian Journal of Crop Science, 2014, 8: 208-214
[8] Hayward AC. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 1991, 29: 65-87
[9] Singh D, Yadav DK, Sinha S, et al. Effect of temperature, cultivars, injury of root and inoculums load of Ralstonia solanacearum to cause bacterial wilt of tomato. Archives of Phytopathology and Plant Protection, 2014, 47: 1574-1583
[10] 何礼远, 康耀卫. 植物青枯菌(Pseudomonas solanacearum)致病机理. 云南农业大学学报, 1995, 10(2): 190-191
[11] 潘晓英, 张振臣, 袁清华, 等. 植物抗青枯病的分子机制研究进展. 植物生理学报, 2022, 58(4): 607-621
[12] Galán JE, Collmer A. Type Ⅲ secretion machines: Bacterial devices for protein delivery into host cells. Science, 1999, 284: 1322-1328
[13] Hueck CJ. Type Ⅲ protein secretion systems in bacterial pathogens of animals and plants. Microbiology and Molecular Biology Reviews, 1998, 62: 379-433
[14] 张勇, 李牧原, 罗锋. 青枯菌三型分泌系统研究进展. 微生物学报, 2015, 55(6): 675-682
[15] Chen K, Khan RAA, Cao W, et al. Sustainable and ecofriendly approach of managing soil born bacterium Ralstonia solanacearum (Smith) using dried powder of Conyza canadensis. Pathogens, 2020, 9: 327
[16] Chen S, Qi GF, Ma GQ, et al. Biochar amendment controlled bacterial wilt through changing soil chemical properties and microbial community. Microbiological Research, 2020, 231: 126373
[17] 蔡秋华, 左进香, 李忠环, 等. 抗性烤烟品种根际微生物数量及功能多样性差异. 应用生态学报, 2015, 26(12): 3766-3772
[18] 易永丰, 周洁尘. 浅谈植物源杀菌剂. 林业与生态, 2018(10): 31-32
[19] Yang L, Wang Y, He XB, et al. Discovery of a novel plant-derived agent against Ralstonia solanacearum by targeting the bacterial division protein FtsZ. Pesticide Biochemistry and Physiology, 2021, 177: 104892
[20] Han ST, Yang L, Wang Y, et al. Preliminary studies on the antibacterial mechanism of a new plant-derived compound, 7-methoxycoumarin, against Ralstonia solanacearum. Frontiers in Microbiology, 2021, 12: 697911
[21] 曹鹏飞. 3种植物提取物对樱桃番茄青枯病病原菌的抑菌活性. 江苏农业科学, 2014, 42(11): 169-170
[22] 杨斌, 陈功锡, 唐克华, 等. 海金沙提取物抑菌活性研究. 中药材, 2011, 34(2): 267-272
[23] 高莹. 紫甘薯叶多糖和黄酮的提取及抑菌作用研究. 硕士论文. 天津: 天津商业大学, 2007
[24] 殷彩霞, 谢家敏, 张更, 等. 茶多酚抑菌抗氧性能研究. 云南化工, 1999(2): 24-26
[25] 曹鹏飞, 陈银华, 周慧娟, 等. 抗青枯病病菌植物杀菌剂的研究. 江苏农业科学, 2017, 45(22): 102-107
[26] 李丽萍. 紫茎泽兰提取物对细菌的抑制作用及抑菌机理的研究. 硕士论文. 北京: 北京林业大学, 2010
[27] 刘朦, 王琦, 鲁一薇, 等. 紫茎泽兰提取物对番茄病害致病菌的抑制作用. 山西农业科学, 2020, 48(5): 784-788
[28] Li P, Yang ZY, Tang BL, et al. Identification of xanthones from the mangosteen pericarp that inhibit the growth of Ralstonia solanacearum. ACS Omega, 2020, 5: 334-343
[29] 朱将伟. 生物农药的应用与研究进展——生防菌及其相关的生物农药. 绿色科技, 2019(22): 203-205
[30] 刘亚苓, 于营, 雷慧霞, 等. 植物病害生防因子的作用机制及应用进展. 中国植保导刊, 2019, 39(3): 23-28
[31] 周开拓, 蒋承耿, 王秋萍, 等. 中生菌素对贵州烟区烟草青枯病的毒力测定及田间防效试验. 农业科技与装备, 2017(11): 14-16
[32] 朱昌雄, 蒋细良, 孙东园, 等. 新农用抗生素——中生菌素. 精细与专用化学品, 2002(16): 14-17
[33] 农业农村部农药检定所药情信息处. 农药登记数据[DB/OL]. (2018-02-23)[2021-07-01]. http://www.icama.org.cn/hysj/index.jhtml
[34] 赵德天, 翟恩昱, 赵啸宇, 等. 设施农业中微生物农药施用情况及发展前景. 生物资源, 2019, 41(3): 195-203
[35] El-Shanshoury AERR, El-Sououd SMA, Awadalla OA, et al. Effects of Streptomyces corchorusii, Streptomyces mutabilis, pendimethalin, and metribuzin on the control of bacterial and Fusarium wilt of tomato. Canadian Journal of Botany, 1996, 74: 1016-1022
[36] 黄瑛. 克菌康防治番茄青枯病药效试验. 农业网络信息, 2006(8): 136
[37] 薛泽程. 50%农用链霉素·诺尔霉素可湿性粉剂的研制. 硕士论文. 重庆: 西南大学, 2007
[38] Trigalet A, Trigalet-Demery D. Use of avirulent mutants of Pseudomonas solanacearum for the biological control of bacterial wilt of tomato plants. Physiological and Molecular Plant Pathology, 1990, 36: 27-38
[39] 肖田, 肖崇刚, 邹阳, 等. 青枯菌无致病力菌株对烟草青枯病的控病作用初步研究. 植物保护, 2008, 34(2): 79-82
[40] 冯吉, 黎妍妍, 程玲, 等. 烟草青枯病的生物防治研究进展. 安徽农业科学, 2016, 44(1): 203-205, 215
[41] Chen WY, Echandi E. Effects of avirulent bacteriocin-producing strains of Pseudomonas solanacearum on the control of bacterial wilt of tobacco. Plant Pathology, 1984, 33: 245-253
[42] 康耀卫, 毛国璋, 吕常胜, 等. 利用青枯菌胞外蛋白输出缺失突变体防治番茄青枯病的研究. 植物保护学报, 1995, 22(3): 287-288
[43] Ongena M, Jacques P. Bacillus lipopeptides: Versatile weapons for plant disease biocontrol. Trends in Microbio-logy, 2008, 16: 115-125
[44] 龙良鲲, 肖崇刚, 窦彦霞. 防治番茄青枯病内生细菌的分离与筛选. 中国蔬菜, 2003(2): 19-21
[45] 曹宇, 陈鹏泽, 曹秀兰, 等. 贝莱斯芽孢杆菌HNU24高效拮抗茄雷尔氏菌和促进植物生长活性的研究. 海南师范大学学报: 自然科学版, 2022, 35(1): 50-56
[46] 余成鹏, 胡蓉花, 陈小强, 等. 江西和广东烟草青枯菌对噬菌体的敏感性及聚类分析. 江西农业大学学报, 2018, 40(4): 699-707
[47] Fujiwara A, Fujisawa M, Hamasaki R, et al. Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages. Applied and Environmental Microbiology, 2011, 77: 4155-4162
[48] Murugaiyan S, Bae JY, Wu J, et al. Characterization of filamentous bacteriophage PE226 infecting Ralstonia solanacearum strains. Journal of Applied Microbiology, 2011, 110: 296-303
[49] Ahmad AA, Stulberg MJ, Mershon JP, et al. Molecular and biological characterization of øRs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum. PLoS One, 2017, 12(9): e0185034
[50] Biosca EG, Català-Senent JF, Figàs-Segura À, et al. Genomic analysis of the first european bacteriophages with depolymerase activity and biocontrol efficacy against the phytopathogen Ralstonia solanacearum. Viruses, 2021, 13: 2539
[51] da Silva XA, da Silva FP, Vidigal PMP, et al. Genomic and biological characterization of a new member of the genus Phikmvvirus infecting phytopathogenic Ralstonia bacteria. Archives of Virology, 2018, 163: 3275-3290
[52] Kawasaki T, Shimizu M, Satsuma H, et al. Genomic characterization of Ralstonia solanacearum phage φRSB1, a T7-like wide-host-range phage. Journal of Bacteriology, 2009, 191: 422-427
[53] Buttimer C, McAuliffe O, Ross RP, et al. Bacteriophages and bacterial plant diseases. Frontiers in Microbiology, 2017, 8: 34
[54] 杨婷婷. 利用番茄相关细菌防治番茄青枯病的筛选策略研究. 硕士论文. 南京: 南京农业大学, 2009
[55] 王杰, 龙世芳, 王正文, 等. 番茄青枯病防治研究进展. 中国蔬菜, 2020(1): 22-30
[56] Mamphogoro TP, Babalola OO, Aiyegoro OA. Sustainable management strategies for bacterial wilt of sweet peppers (Capsicum annuum) and other Solanaceous crops. Journal of Applied Microbiology, 2020, 129: 496-508
[57] 陈志迪, 王新宇, 李晴雯, 等. 植物源抑菌剂的研究进展. 食品安全质量检测学报, 2021, 12(18): 7433-7439
[58] 李晓菲, 徐政. 植物源杀菌剂研究进展. 南方农业, 2018, 12(13): 40-42
[59] 贾睿, 蔡丹, 刘景圣, 等. 天然植物源抑菌活性成分研究进展. 食品工业, 2020, 41(10): 283-287
[60] 王宇, 金剑雪, 李凤良. 微生物农药在植物病虫害防治中的应用策略. 现代农机, 2022(5): 107-109
[61] 沈志松, 金坚, 陈蕴. 一种微生物新农药在防治作物纹枯病中的应用. 中国. CN112335684A. 2021-02-09
[62] 崔佳佳, 张雪洪. 微生物源农用抗生素的研发与高产策略. 生物工程学报, 2021, 37(3): 1032-1041
[63] 王震. 水培番茄青枯病发生条件及防治研究. 硕士论文. 武汉: 华中农业大学, 2008
[64] Arwiyanto T, Goto M, Tsuyumu S, et al. Biological control of bacterial wilt of tomato by an avirulent strain of Pseudomonas solanacearum isolated from Strelitzia reginae. Japanese Journal of Phytopathology, 1994, 60: 421-430
[65] 陈国康, 周帮菊, 周丹妮, 等. 无致病力青枯菌株对烟草青枯病的控制作用. 烟草科技, 2015, 48(11): 7-10
[66] 邱敬萍, 黄艳霞, 王超, 等. EG03菌剂对辣椒青枯病的防治效果及对根围土壤微生物群落的影响. 应用生态学报, 2014, 25(5): 1468-1474
[67] 宫超, 黎振兴, 麦培婷, 等. 番茄青枯病抗性相关根际微生物的研究进展. 广东农业科学, 2021, 48(9): 51-61
[68] 高芬, 郝锐, 秦雪梅, 等. 防治药用植物土传病害的芽胞杆菌制剂开发的制约因素分析. 植物保护, 2017, 43(3): 23-28
[69] 陈志谊, 刘邮洲, 刘永锋, 等. 拮抗细菌菌株之间的互作关系及其对生物防治效果的影响. 植物病理学报, 2005, 35(6): 539-544
[70] Ramírez M, Neuman BW, Ramírez CA. Bacteriophages as promising agents for the biological control of Moko disease (Ralstonia solanacearum) of banana. Biological Control, 2020, 149: 104238
[71] Thomas RC. A bacteriophage in relation to Stewart’s disease of corn. Phytopathology, 1935, 25: 371-372
[72] Iriarte FB, Balogh B, Momol MT, et al. Factors affecting survival of bacteriophage on tomato leaf surfaces. Applied and Environmental Microbiology, 2007, 73: 1704-1711
[73] 王佳宁, 王玉鑫, 侯如娇, 等. 噬菌体鸡尾酒联合生物有机肥防控番茄青枯病的效果研究. 微生物学通报, 2021, 48(9): 3194-3204
[74] Iriarte FB, Obradović A, Wernsing MH, et al. Soil-based systemic delivery and phyllosphere in vivo propagation of bacteriophages: Two possible strategies for improving bacteriophage persistence for plant disease control. Bacteriophage, 2012, 2: 215-224

番茄青枯病生物防治的研究进展

Research progress in biological control of tomato bacterial wilt

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李晓芳, 田叶韩, 彭海莹, 何邦令, 高克祥. 防治苦瓜枯萎病的拮抗放线菌分离筛选及鉴定 [J]. 应用生态学报, 2020, 31(11): 3869-3879.
[2]	李健, 李肖鹤, 后文, 郑沈, 朱向东. 榕树根际土壤广谱拮抗菌株的筛选、鉴定及特性 [J]. 应用生态学报, 2019, 30(11): 3894-3902.
[3]	李培谦,冯宝珍,李新秀,郝浩永. 番茄灰霉菌拮抗放线菌LA-5的筛选及鉴定 [J]. 应用生态学报, 2018, 29(12): 4172-4180.
[4]	刘丽英,丁文龙,曹雅杰,尤鸿基,刘珂欣,孙中涛,毛志泉. 苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响 [J]. 应用生态学报, 2018, 29(12): 4165-4171.
[5]	张好好, 刘振国, 龚佑辉, 刁青云. 信息素介导的西方蜜蜂与狄斯瓦螨互作研究进展 [J]. 应用生态学报, 2017, 28(6): 2055-2062.
[6]	刘英杰, 迟宝杰, 林芳静, MAIGRETOlivier, 郑鹭飞, 刘勇. 反-β-法尼烯对马铃薯蚜虫及其天敌的生态效应 [J]. 应用生态学报, 2016, 27(8): 2623-2628.
[7]	赵龙飞, 徐亚军, 侯怡婷, 邹艳慧, 李亚楠, 杨志华, 李小雨, 张梦瑶. 烟草赤星病菌拮抗性大豆根瘤内生菌的筛选及抑制作用 [J]. 应用生态学报, 2016, 27(5): 1560-1568.
[8]	申红妙, 李正楠, 贾招闪, 杨佳瑶, 冉隆贤. 内生枯草芽孢杆菌JL4在葡萄叶上的定殖及其对葡萄霜霉病的防治 [J]. 应用生态学报, 2016, 27(12): 4022-4028.
[9]	段曦, 毕焕改, 魏佑营, 李婷, 王洪涛, 艾希珍. 嫁接对辣椒根际土壤环境的影响及其与抗病性和产量的关系 [J]. 应用生态学报, 2016, 27(11): 3539-3547.
[10]	薛应钰, 范万泽, 张树武, 徐秉良. 苹果树腐烂病菌拮抗放线菌的筛选、鉴定及防效 [J]. 应用生态学报, 2016, 27(10): 3379-3386.
[11]	吕恒1, 2,牛永春2**,邓晖2,林晓民1,金春丽2. 根际真菌对黄瓜土传病害的抑制作用 [J]. 应用生态学报, 2015, 26(12): 3759-3765.
[12]	杨蓓芬1,2**,杜乐山3,李钧敏1,2. 南方菟丝子寄生对加拿大一枝黄花生长、繁殖及防御的影响 [J]. 应用生态学报, 2015, 26(11): 3309-3314.
[13]	贺张,刘继兵,陈永玲,陈中正,段毕升,胡好远**. 不同方法处理的家蝇蛹对蝇蛹金小蜂繁殖的影响 [J]. 应用生态学报, 2013, 24(3): 795-800.
[14]	赵紫华,贺达汉,杭佳,石云,赵映书,王颖. 设施农业景观下破碎化麦田麦蚜及寄生蜂种群的最小适生面积 [J]. 应用生态学报, 2011, 22(01): 206-214.
[15]	梅新兰,赵青云,谭石勇,徐阳春,沈标,沈其荣. 辣椒疫病拮抗菌株筛选、鉴定及其防效 [J]. 应用生态学报, 2010, 21(10): 2652-2658.