利福霉素生产菌产生钝化Rif SV物质的分离及性质研究

应用生态学报 ›› 1995, Vol. 6 ›› Issue (3): 282-286.

利福霉素生产菌产生钝化Rif SV物质的分离及性质研究

吴文芳, 张成刚, 吕安国, 张立新, 胡江春, 曾青

中国科学院沈阳应用生态研究所, 沈阳 110015

收稿日期:1995-03-11 修回日期:1995-06-02 出版日期:1995-07-25 发布日期:1995-07-25
基金资助:
中国科学院重点资助

Isolation and properties or Rifamycin SV-inactivation substance produced by Rif SV-pro-ductive strain

Wu Wenfang, Zhang Chenggang, Lü Anguo, Zhang Lixin, Hu Jiangchun, Zeng Qing

Institute of Applied Ecology, Academia Sinica, Shenyang 110015

Received:1995-03-11 Revised:1995-06-02 Online:1995-07-25 Published:1995-07-25

摘要/Abstract

摘要： 利福霉素SV(简称Rif SV)生产菌──地中海拟无枝菌酸菌在生物合成RifSV过程中,产生一种能钝化自身产物(RifSV)的物质。实验证实,该物质是由谷氨酸、天冬氨酸、赖氨酸及缬氨酸等14种常见氨基酸组成的蛋白质。分子量(MW)约2.5×10⁴D,等电点(PI)为5.7-6.1.在100℃下加热10min,其活性丧失。作用于RifSV的最适pH值范围为7.4-8.6,最适温度为29℃,初步证实该物质是一种酶(暂称利福霉素钝化酶).

关键词: 地中海拟无枝菌酸菌, 利福霉素SV, 利福霉素钝化酶, 抗生素酶

Abstract: The productive strain(Amycolatopsis mediterranei) produces a substance for inactivating self-product (Rif SV) in the course of Rif SV-biosynthesis.The experiments demonstrate that it is a protein consisting of 14 kinds of common amino acids including Gin, Asp,Lys, Val and so on.Its molecular weight (MW) is approximately 2.5×10⁴D and its isoelectric point (PI) is 5.7-6.1.After heated at 100℃ for 10 min,its activity is disappeared.The optimum PH range for reacting on Rif SV is 7.4-8.6 and the optimum temperature is 29℃.It is preliminarily identified as a enzyme (temporarily designated as Rifamycin SV-inactivation enzyme).

Key words: Amycolatopsis mediterranei, Rifamycin SV, Rifamycin SV-inactivation enzyme, Antibiotic enzyme

吴文芳, 张成刚, 吕安国, 张立新, 胡江春, 曾青. 利福霉素生产菌产生钝化Rif SV物质的分离及性质研究[J]. 应用生态学报, 1995, 6(3): 282-286.

Wu Wenfang, Zhang Chenggang, Lü Anguo, Zhang Lixin, Hu Jiangchun, Zeng Qing. Isolation and properties or Rifamycin SV-inactivation substance produced by Rif SV-pro-ductive strain[J]. Chinese Journal of Applied Ecology, 1995, 6(3): 282-286.