植物根际促生菌(PGPR)的研究与应用前景

摘要/Abstract

摘要： 植物土传病害难以防治,植物根际促生菌(plant growth-promoting rhizobacteria,PGPR)的深入研究和发展为解决这一难题展现了诱人的前景.PGPR能够高密度地在植物根际定殖,兼有抑制植物病原菌、根际有害微生物,以及促进植物生长并增加作物产量的作用,更重要的是有些PGPR能够诱导植物产生系统抗性(induced systemic resistance,ISR),从而提高植物整体的抗病能力.近20年来,国外这一领域的研究十分活跃,已有很多成功应用的PGPR产品,国内应大力加强基础与应用的研究,并推进其产业化的发展.

关键词: 植物根际促生菌(PGPR), 生物防治

Abstract: The study of plant growth-promoting rhizobacteria will provide possible methods to overcome the difficulties in controlling soil borne plant diseases.PGPR can colonize in rhizosphere at high population density,inhibit plant pathogens and deleterious microorganisms there,and promote crop growth and its yield.More importantly,some PGPR strains applied as biocontrol agents can make treated plant produce induced systemic resistance (ISR),and thus,increases plant overall health.In recent two decades,the researches of PGPR in abroad are very active,and many PGPR products have been applied successfully.In our contry,more attention should be paid to the study of the basic theory and application of GPPR and the industrialization of PGPR products.

Key words: PGPR, Biocontrol agent

中图分类号:

S432.4

胡江春, 薛德林, 马成新, 王书锦. 植物根际促生菌(PGPR)的研究与应用前景[J]. 应用生态学报, 2004, (10): 1963-1966.

HU Jiangchun, XUE Delin, MA Chengxin, WANG Shujin. Research advances in plant growth-promoting rhizobacteria and its application prospects[J]. Chinese Journal of Applied Ecology, 2004, (10): 1963-1966.

参考文献

[1] Bakker PA HM, Ran LX, Pieterse CMJ, et al. 2003. Understanding the involvement of rhizobacteria-mediated induction of systemic resistance in biocontrol of plant diseases. Can J Plant Patho, 25: 5～9
[2] Broadbent P, et al. 1971. Bacteria and actinomycetes antagonistic to fungal root pathogens in Australian soil. Aust J Biol Sci, 24: 925～944
[3] Burdman S, Jurkevitch E, Okon Y, et al. 2000. Recent advances in the use of plant growth promoting Rhizobacteria(PGPR)in agriculture. In: Subba Rao NS, Dommergues YR. eds. Microbial Interactions in Agriculture and Forestry. Calicut, India: Science Publishers Inc. 229 ～ 250
[4] Chen X-B(陈晓斌),Zhang B-X(张炳欣),Lou B-G(娄兵干),et al.2001.Introduction ofthe chromogenic gene tothe plant growthpromoting rhizobacteria of cucumber.Acta Microbiol Sin(微生物学报),41:287 292(in Chinese)
[5] Chen X-B(陈晓斌)and Zhang B-X(张炳欣).2000.The proceedings of PGPR mechanisms.JMicrobiol(微生物学杂志),20:38～41(in Chinese)
[6] DingY-Q(丁延芹)and Du B-H(杜秉海).2001.Screening of PGPR strains isolated from the rhizosphere of maize. Soil Fert (土壤肥料), 3:41～43(in Chinese)
[7] El Ghaouth A, Wilson CL and Wisniewski M. 2003. Control of postharvest decay of apple with Candida saitoana and induction of defense responses. Phytopathology, 93: 344 ～ 348
[8] He CY, Hsiang T and Wolyn DJ. 2002. Induction of systemic disease and pathogen defense responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium infection. Plant Pathol, 51: 225 ～ 230
[9] Hu B-F(胡炳福 ). 2000. The application of PGPR on chinese forest. Guizhou For Sci Technol ( 贵州林业科技 ), 28: 41 ～ 47 ( in Chinese)
[10] Hu J-C(胡江春),Xue D-L(薛德林),Wang S-J(王书锦), et al.2002. Obstacles of soybean continuous cropping Ⅲ. Mechanism of soybean yield increment by marine actinomyces MB-97. Chin J Appl Ecol (应用生态学报), 13:1095 ～ 1098(in Chinese)
[11] Jin H, et al. 1996. Characterization and purification of a phytotoxin produced by Fusarium solani, the causal agent of soybean sudden death syndrome. Phytopathology, 86: 277～ 282
[12] Jetiyanon K and Kloepper JW. 2002. Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biol Control, 24: 285 ～ 291
[13] Kloepper JW, Rodriguez-Ubana GW, Zehnder B, et al. 1999. Plant root bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Aust Plant Pathol, 28: 21 ～ 26
[14] Kloepper JW, Leong T, Teintze M, et al. 1980. Enhanced plant growth by siderphones produced by plant growth-promoting rhizobacteria. Nature, 286: 885 ～ 886
[15] Lalitha B. 1989. Phytotoxin produced by Diaporthe phaseolorurn var. caulivora, the causal organism of stem canker of soybean. Phytopathology, 79: 499 ～ 504
[16] Long W-W (龙伟文), Wang P ( 王平), Feng X-M ( 冯新梅).2000. Research progress on PGPR/AMF interactions. Chin J Appl Ecol(应用生态学报), 11(2) :311 ～ 314(in Chinese)
[17] Penrose DM and Glick BR. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoring rhizobacteria. Physiol Plantarum, 118:10～ 15
[18] Ramamoorthy V, Viswanathan R, Raguchander T, et al. 2001. Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protec, 20:1 ～11
[19] Ramamoorthy V, Raguchander T and Sarniyappan R. 2002. Enhancing resistance of tomato and hot pepper to Pythium diseases by seed treatment with Fluorescent pseudornonads. Euro J Plant Pathol, 108: 429～ 441
[20] Rangeshwaran R, Wasnikar AR, Prasad PD, et al. 2002. Isolation of endophytic bacteria for biological control of wilt pathogens. J Biol Control, 16:125～134
[21] Ryu CM, Farag MA, Hu CH, et al. 2003. Bacterial volatiles promote growth in Arabidlopsis. Proc Natl Acad Sci USA, 100:4927～ 4932
[22] Silva H and Sandro A. 2003. Development of a root colonization bioassay for rapid screening of rhizobacteria for potential biocontrol agents. J Phytopathol, 151: 42 ～ 46
[23] Schippers B, Bakker AW, Backker PA. 1987. Interactions of deleterious and beneficial rhizosphere microoganisms. Annu Rev Phytopathol, 25: 339 ～ 358
[24] Someya N, Nakajima M, Hibi T, et al. 2002. Induced resistance to rice blast by antagonistic bacterium, Serratia marcescens strain B2.J Gen Plant Pathol, 68:177～ 182
[25] Stockwell VO, Moore LW, Loper JE. 1993. Fate of Agrobacterium radiobacter K84 in the environment. Appl Environ Microbiol, 59 :2112～2120
[26] Wang C-X(王春霞). 1998. Studies on isolation selection, identification, mechanisms and molecular ecology of the PGPR strains in cotton. Acta Phytopathol Sin (植物病理学报), 28:190～ 191(in Chinese)
[27] Wang J-S(王金生), Zhao L-P(赵立平), Feng J-X(冯家勋).2001. Recombination microorganisms for biological control of plant disease. In:Huang D-F(黄大昉 ), eds. Genetic Engineering of Agricultural Microorganisms. Beijing: Science Press. 316 ～ 408
[28] Wang p(王平)and Feng X-M(冯新梅). 1999. Screening and identification of PGPR strains isolated from the rhizosphere of winter wheat( Triticum aestivum L. ). J Huazhong Agric Univ ( 华中农业学学报), 18:352～356(in Chinese)
[29] Weller DM. 1988. Biological control of soilborhe plant pathogen in the rhizophere with bacteria. Ann Rev Phytopathol, 26:379～407
[30] Yao CB, Zehner GW, Sikora E, et al. 1997. Evaluation of induced systemic resistance and plant growth promotion in tomato with selected PGPR strains. In: Ogoshi A, Kobayashi K, Homma Y, eds.Plant Growth-Promoting Rhizobacteria-Present Status and Future Prospects. Sapporo: Nakanishi Printing. 285～ 288
[31] Zehnder G, Klopper J, Yao C, et al. 1997. Induction of systemic resistance in cucumber against cucumber beetles by plant growth-promoting rhizobacteria. J Econ Entomol, 90: 391 ～ 396
[32] Zhang L, Wu WM, Yan ZN, et al. 1996. Research and commercialization of yield-increasing bacteria(YIB)in China. In Tang WH,Cook RJ, and Rovira, eds. Advances in Biological Control of Plant Disease. Beijing: China Agricultural University Press. 47 ～ 53
[33] Zhang S, Reddy MS and Kloepper JW. 2002. Development of assays for assessing induced systemic resistance by plant growth-promoting rhizobacteria against blue mold of tobacco. Biol Control, 23: 79 ～86