UV-B辐射增强对三种赤潮微藻DNA的伤害效应

应用生态学报 ›› 2005, Vol. 16 ›› Issue (3): 559-562.

UV-B辐射增强对三种赤潮微藻DNA的伤害效应

蔡恒江¹, 唐学玺¹, 张培玉^1,2

1. 中国海洋大学海洋生态学研究室, 青岛, 266003;
2. 曲阜师范大学生命科学学院, 曲阜, 273165

收稿日期:2004-03-24 修回日期:2004-07-05 出版日期:2005-03-15 发布日期:2005-03-15
通讯作者: 唐学玺
基金资助:
国家自然科学基金项目(30270258)和山东省优秀中青年科学家科研奖励基金资助项目(03BS120).

Effects of UV-B radiation enhancement on DNA damage of three red-tide microalgae species

CAI Hengjiang¹, TANG Xuexi¹, ZHANG Peiyu^1,2

1. Marine Ecology Laboratory, Ocean University of China, Qingdao 266003, China;
2. College of Life Sciences, Qufu Normal University, Qufu 273165, China

Received:2004-03-24 Revised:2004-07-05 Online:2005-03-15 Published:2005-03-15

摘要/Abstract

摘要： 运用生态毒理学和生物化学方法研究了UV-B辐射增强对赤潮异弯藻、亚历山大藻和中肋骨条藻DNA的伤害作用.结果表明,3种赤潮微藻的生长状况对UV-B辐射增强的敏感性不同;对UV-B辐射增强的敏感性由高到低依次是赤潮异弯藻、亚历山大藻和中肋骨条藻.随着UV-B辐射剂量的增加,3种赤潮微藻的DNA损伤程度提高,而且赤潮异弯藻DNA的损伤程度明显高于亚历山大藻和中肋骨条藻,亚历山大藻DNA的伤害程度又远远高于中肋骨条藻.UV-B辐射处理解除后,损伤DNA可明显恢复.赤潮异湾藻和亚历山大藻恢复培养6d,损伤DNA可明显恢复(P＜0.05);而中肋骨条藻恢复培养3d,损伤DNA可明显恢复(P＜0.05),说明3种赤潮微藻的DNA损伤水平不适合作为指示UV-B辐射增强的生物学指标.

关键词: 赤潮异弯藻, 亚历山大藻, 中肋骨条藻, UV-B辐射增强, DNA损伤

Abstract: The ecotoxicological and biochemical study with Heterosigma akashiwo,Alexandrium tamarense and Skeletonema costatum showed that the test three species of red tide microalgae had different sensitivities to UV B radiation enhancement,and the order from high to low was Heterosigma akashiwo,Alexandrium tamarense and Skeletonema costatum.The DNA damage of algal cells increased with UV B radiation enhancement,being more notable in Heterosigma akashiwo than in Alexandrium tamarense and Skeletonema costatum,and in Alexandrium tamarense than in Skeletonema costatum.The DNA damage of Heterosigma akashiwo and Alexandrium tamarense could be significantly repaired (P<0.05) after 6 days of UV B radiation removal,and that of Skeletonema costatum could be significantly repaired (P<0.05) after 3 days,which illustrated that the DNA damage of three red tide microalgae species could not indicate the damaged degree of marine microalgae resulted from UV B radiation enhancement.

Key words: Heterosigma akashiwo, Alexandrium tamarense, Skeletonema costatum, UV-B radiation enhancement, DNA damage

中图分类号:

X171.5

蔡恒江, 唐学玺, 张培玉. UV-B辐射增强对三种赤潮微藻DNA的伤害效应[J]. 应用生态学报, 2005, 16(3): 559-562.

CAI Hengjiang, TANG Xuexi, ZHANG Peiyu. Effects of UV-B radiation enhancement on DNA damage of three red-tide microalgae species[J]. Chinese Journal of Applied Ecology, 2005, 16(3): 559-562.

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