小球藻细胞活性物质的提取及对啤酒酵母的生理效应

应用生态学报 ›› 2005, Vol. 16 ›› Issue (8): 1573-1576.

小球藻细胞活性物质的提取及对啤酒酵母的生理效应

胡开辉, 周山勇

福建农林大学生命科学学院, 福州 350002

收稿日期:2004-08-16 修回日期:2004-12-15 出版日期:2005-08-15 发布日期:2005-08-15
通讯作者: 胡开辉,男,1962年生,副教授.主要从事微生物生态和食用真菌的教学与科研工作,发表论文8篇.E-mail:hukh62@sohu.com
基金资助:
国家自然科学基金项目(30471028、30200170)和福建省教育厅资助项目(K04042).

Extraction of active substance from Chlorella vulgaris cells and its physiological effects on Saccharomyces cerevisiae

HU Kaihui, ZHOU Shanyong

School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2004-08-16 Revised:2004-12-15 Online:2005-08-15 Published:2005-08-15

摘要/Abstract

Abstract: The study showed that freezing-thawing method could have a higher cellwall breakage rate,and a light injury to the active substance,which could not only increase the cell fission and growth,delay the death of Saccharomycete cells,but also enhance CO₂ production rate and quicken fermentation process when the cells were exposed to the medium with 0.5% active substance.

Key words: Chlorella vulgaris, Active substance, Extraction, Saccharomycete, Physiological effects

中图分类号:

TS262

胡开辉, 周山勇. 小球藻细胞活性物质的提取及对啤酒酵母的生理效应[J]. 应用生态学报, 2005, 16(8): 1573-1576.

HU Kaihui, ZHOU Shanyong . Extraction of active substance from Chlorella vulgaris cells and its physiological effects on Saccharomyces cerevisiae[J]. Chinese Journal of Applied Ecology, 2005, 16(8): 1573-1576.

参考文献

[1] Borowitka M.1992.Mircoalgal Biotechnology.Cambridge:Cambridge University Press.254～287
[2] Cao J(曹键),Zhou Z-J(周志军).1997.Nutrient value and exploitation of production and application of microalgal.Cereal Feed Ind(粮食与饲料工业),5:28～31(in Chinese)
[3] Chen F(陈峰),Jiang Y(姜悦).1999.Biotechnology of Microalgal.Beijing:China Light Industry Press.(in Chinese)
[4] Chen Y(陈颖).1998.Progress in the studies on Cholrella biotechnology andits prospect.Proc Biotech nol(生物工程进展),9(6):12～16(in Chinese)
[5] de Souza QJ,Malacrida SA,Justo GZ,et al.2004.Myelopoietic response in mice exposed to acute cold/restraint stress:Modulation by Chlorella vulgaris prophylactic treatment.Immunopharmacol Immunotoxicol,26(3):455～467
[6] Dong X-W(董秀文),Li Y(李岩).2003.Application of SPSS in statistical analysis of physical and chemical experimental data.Physic Testing Chem Anal:Physic Anal(理化检验:物理分册),39(11):574～577(in Chinese)
[7] El-Naggar AH,El-Sheekh MM.1998.Abolishing cadmium toxicity in Chlorella vulgaris by ascorbic acid,calcium,glucose and reduced glutathione.Environ Poll,101(2):169～174
[8] Han S-Q(韩士群),Chang Z-C(常志川),Zheng Q(郑勤).2001.Effect of cholrella growth factor on leavening of beer and Lactic Acid Products.Food Sci(食品科学),22(10):54～56(in Chinese)
[9] Han S-Q(韩士群),Zhang Z-H(张振华),Liu H-Q(刘海琴).2003.Physiological function of Chlorella growth factor.Jiangsu Agric Sci(江苏农业科学),5:99～101(in Chinese)
[10] Kozlowska SB,Bialuk I,Maleszewski S.2004.Enhancement of photosynthetic O₂ evolution in Chlorella vulgaris under high light and increased CO₂ concentration as a sign of acclimation to phosphate deficiency.Plant Physiol Biochem,42(5):403～409
[11] Li S-W(李师翁),Li H(李欢).1997.The resource of single plant cell protein-Current status of studies on Cholrella exploitation and application.Biotechnology(生物技术),7(3):45～49(in Chinese)
[12] Lin Y-C(林永成),Zhou S-N(周世宁).2003.Marine Microorganisms and their Metabolic Products.Beijing:Science Press.(in Chinese)
[13] Lintongan PB,Carino FA,Rivero GC.2004.Photosynthetic protein from Chlorella vulgaris strain Bt-09 may be responsible for the coping mechanism against cadmium toxicity.Bull Environ Contam Toxicol,72(6):1232～1239
[14] Liu R-X(刘润幸).2003.ROC curve analysis of multivariable observation value using SPSS.Chin J Public Health(中国公共卫生),19(9):1151～1152(in Chinese)
[15] Makoto K,Takashi Y,Kouichirou M,et al.1998.Degradation of Chlorella cells during composting.J Biotechnol,66(2～3):187～193
[16] Mallick N.2004.Copper-induced oxidative stress in the chlorophycean microalga Chlorella vulgaris:Response of the antioxidant system.J Plant Physiol,161(5):591～597
[17] Mukherjee I,Gopal M,Dhar DW.2004.Disappearance of chlorpyrifos from cultures of Chlorella vulgaris.Bull Environ Contam Toxicol,73(2):358～363
[18] Netzeva TI,Dearden JC,Edwards R,et al.2004.Toxicological evaluation and QSAR modelling of aromatic amines to Chlorella vulgaris.Bull Environ Contam Toxicol,73(2):385～391
[19] Peredo-Alvarez VM,Sarma SS,Nandini S.2003.Combined effect of concentrations of algal food(Chlorella vulgaris)and salt(sodium chloride)on the population growth of Brachionus calyciflorus and Brachionus patulus(Rotifera).Rev Biol Trop,51(2):399～407
[20] Ren J-L(任景玲),Liu S-M(刘素美),Zhang J(张经),et al.2003.Effects of terrestrial input on the harmful algal bloom areawith aluminum as an example.Chin J Appl Ecol(应用生态学学报),14(7):1117～1121(in Chinese)
[21] Singh A,Sharma L,Mallick N.2004.Antioxidative role of nitric oxide on copper toxicity to a chlorophycean alga Chlorella.Ecotoxicol Environ Saf,59(2):223～227
[22] Sun W-J(孙王杰),Zhan X-Q(战学秋).2003.Using statistical software SPSS to analyze students' scores.J Jilin Inst Chem Technol(吉林化工学院报),20(3):87～89(in Chinese)
[23] Umamaheswari A,Venkateswarlu K.2004.Impact of nitrophenols on the photosynthetic electron transport chain and ATP content in Nostoc muscorum and Chlorella vulgaris.Ecotoxicol Environ Sa f,58(2):256～259
[24] Wang J-H(王金辉),Huang X-Q(黄秀清).2003.Ecological characteristics of Prorocentrum dentatum and the cause of harmful algal bloom formation in China Sea.Chin J Appl Ecol(应用生态学报),14(7):1065～1069(in Chinese)
[25] Wang W(王微).1997.Current situation of plant proteins production and processing at home and abroad.Food Sci(食品科学),18(8):3～7(in Chinese)
[26] Yamamoto M,Fujishita M,Hirata A,et al.Regeneration and maturation of daughter cell walls in the autospore-forming green alga Chlorella vulgaris(Chlorophyta,Trebouxiophyceae).J Plant Res,117(4):257～264
[27] Yan X(严雪),Yang Y-Q(杨永清),Li Y-K(李永科),et al.2002.Accumulation and biodegradation of anthracene by Chlorella protothecoides under different trophic conditions.Chin J Appl Ecol(应用生态学报),13(2):145～150(in Chinese)
[28] Yang L-S(杨鹭生),Li G-P(李国平),Chen L-S(陈林水).2003.Analysis of the contents of protein and amino acid in the powder of Chlorella pyrenoidosa and its nutritive value.Subtrop Plant Sci(亚热带植物科学),32(1):36～38(in Chinese)
[29] Yang Z-Q(杨志强),Dong B(董波),Wu J-C(吴进才).2004.Sensitivity of Chlorella vulgaris to metribuzin,puma and alachlor.Chin J Appl Ecol(应用生态学报),15(9):1621～1625(in Chinese)
[30] Yoshida T,Hairston NG Jr,Ellner SP.2004.Evolutionary trade-off between defence against grazing and competitive ability in a simple unicellular alga,Chlorella vulgaris.Proc R Soc Lond B Biol Sci,271(1551):1947～1953
[31] Zheng Y(郑怡),Yu P(余萍),Liu Y-R(刘艳如).2003.Purification and partial characterization of Chlorella pyrenoidosa lectin.Acta Hydrobiol Sin(水生生物学报),27(1):36～40(in Chinese)