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青海盐湖嗜盐微生物类群及F16菌株生长特性和抗菌、抗肿瘤活性

叶央芳;严小军;黄晓春;陈烨;陈海敏;朱世华   

  1. 宁波大学生命科学与生物工程学院, 宁波 315211
  • 收稿日期:2005-09-30 修回日期:2006-07-26 出版日期:2006-10-18 发布日期:2006-10-18

Halophilous microbial groups in saline lake of Qinghai and the growth characteristics and anti-microbial and anti-tumor activities of F16.

YE Yangfang; YAN Xiaojun;HUANG Xiaochun; CHEN Ye;CHEN Haimin; ZHU Shihua   

  1. College of Life Science and Biotechnology, Ningbo University, Ningbo 315211,China
  • Received:2005-09-30 Revised:2006-07-26 Online:2006-10-18 Published:2006-10-18

摘要: 从青海盐湖底泥中分离出45株嗜盐微生物.其中,丝状真菌F16的抗菌和抗肿瘤活性最强,其发酵液的乙酸乙酯粗提物能抑制4种细菌的生长,尤其对大肠杆菌的抑制作用最强,具有较强的细胞毒活性,当粗提物浓度为50 μg·ml-1时对肝癌细胞BEL7402的抑制率可达76.91%.F16菌株的最适生长温度为15 ℃;培养基盐度升高,对F16的抑制性增强,当盐度超过15%时,F16不生长;在pH值为5~9范围内,F16生长良好.

关键词: 球孢白僵菌, 桃蚜, 特定时间侵染率

Abstract: A total of forty-five halophilous microorganisms were isolated from the sediment of saline lake in Qinghai Province, among which, filamentous fungus F16 showed the highest activity of anti-microorganism and anti-tumor. The ethyl acetate extract of F16 culture filtrate showed a strong cytotoxicity, and could inhibit the growth of four kinds of bacteria, especially Escherichia coli. When the concentration of the crude extract was 50 μg·ml-1, the inhibition rate to liver cancer cell BEL7402 reached 76.91%. The optimal temperature for F16 growth was 15 ℃,and the increase of salt concentration in media would inhibit its growth. When the concentration of salt surpassed 15%, F16 could not survive. F16 grew well when the pH value ranged from 5 to 9.

Key words: Beaveria bassiana, Myzus persicae, Time-specific-infection rate