转Bt基因抗虫棉叶面可培养微生物多样性的变化

应用生态学报

转Bt基因抗虫棉叶面可培养微生物多样性的变化

王元格¹;谭茂玉²;李金娥¹;谢庆恩¹;范作晓¹;沈法富¹

¹山东农业大学农学院山东省作物生物学重点实验室，山东泰安 271018；
²莱芜职业技术学院，山东莱芜 271100

收稿日期:2006-02-23 修回日期:2006-12-26 出版日期:2007-03-18 发布日期:2007-03-18

Changes in diversity of culturable microorganisms on leaf surface of transgenic Bt cotton

WANG Yuan-ge¹; TAN Mao-yu²; LI Jin-e¹; XIE Qing-en¹; FAN Zuo-xiao¹; SHEN Fa-fu¹

¹Key Laboratory of Crop Biology of Shandong Province, College of Agronomy, Shandong Agricultural University, Taian 271018, Shandong, China;
²Laiwu Vocational and Technical College, Laiwu 271100, Shandong, China

Received:2006-02-23 Revised:2006-12-26 Online:2007-03-18 Published:2007-03-18

摘要/Abstract

摘要： 在大田栽培条件下，以转Bt基因抗虫棉GK-12和常规棉泗棉3号为材料，在棉花的苗期、现蕾期、花铃期和吐絮期分别测定棉花叶面细菌、真菌和放线菌的数量变化，并在花铃期和吐絮期对叶面细菌生理群的数量和多样性进行分析.结果表明：棉花叶面可培养微生物数量与其生长发育呈正相关，叶面可培养微生物的数量一般由苗期开始增多，到花铃期达最高峰，吐絮期明显减少；花铃期转Bt基因抗虫棉叶面细菌生理群Simpson指数、Shannon-Wiener指数和均匀度比常规棉升高，吐絮期比常规棉下降.

Abstract: In a field study, the quantitative changes of bacterial, actinomycetic and fungal populations on the leaf surface of transgenic Bt cotton (GK-12) and non-transgenic Bt cotton (Simian No.3) were monitored at seedling, squaring, flowering and boll-opening stages, respectively, and the diversity of bacterial physiological groups was analyzed at flowering and boll-opening stages. The results showed that there was a positive correlation between the quantity of culturable microorganisms and the development of cotton. The total number of bacteria, fungi and actinomyces began to rise at seedling stage, reached its peak at flowering stage, and decreased markedly at boll-opening stage. The Simpson index, Shannon-Wiener index, and evenness index of bacterial physiological groups were higher at flowering stage, but lower at boll-opening stage on GK-12 than on Simian No.3.

Key words: Monopterus albus, Ecological factors, Oosperm, Hatching rate

王元格¹;谭茂玉²;李金娥¹;谢庆恩¹;范作晓¹;沈法富¹. 转Bt基因抗虫棉叶面可培养微生物多样性的变化[J]. 应用生态学报.

WANG Yuan-ge¹; TAN Mao-yu²; LI Jin-e¹; XIE Qing-en¹; FAN Zuo-xiao¹; SHEN Fa-fu¹ . Changes in diversity of culturable microorganisms on leaf surface of transgenic Bt cotton[J]. Chinese Journal of Applied Ecology.