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基因工程菌在活性污泥中的衰减及其影响因素

刘春;黄霞;王慧   

  1. 清华大学环境科学与工程系环境模拟与污染控制国家联合重点实验室, 北京 100084
  • 收稿日期:2006-03-03 修回日期:2007-01-07 出版日期:2007-03-18 发布日期:2007-03-18

Decay of genetically engineered microorganisms (GEMs) in activated sludge and its related affecting factors

LIU Chun; HUANG Xia; WANG Hui   

  1. State Key Joint Laboratory of Environmental Simulation and Pollution Control,Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China
  • Received:2006-03-03 Revised:2007-01-07 Online:2007-03-18 Published:2007-03-18

摘要: 在间歇实验和连续运行的反应器中,考察了基因工程菌细胞密度在活性污泥中的衰减,进行了动力学模型拟合,求得模型参数,并分析了影响衰减的因素.结果表明,在间歇实验和连续运行反应器中,细胞密度在活性污泥中均呈现初期快速衰减而后趋于稳定的趋势.其衰减动力学可以用Logistic模型和Gompertz模型拟合,两个模型的拟合都有很好的相关性和显著性,拟合优度没有差异.在不同的条件下,间歇实验拟合得到的参数取值范围是:Logistic模型衰减系数r′=0.5~0.6 h-1,稳定细胞密度K′=104~105 cfu·ml-1;Gompertz模型初始衰减系数b=0.6~1.2 h-1,比衰减系数a=0.02~0.09 h-1.初始投加密度对衰减速率影响不大,但是显著影响稳定细胞密度.污泥浓度和温度对衰减速率和稳定密度均有显著影响. 污泥浓度越大,衰减越快,稳定密度越大;而温度越低,衰减越快,稳定密度也会越低.营养条件和微型动物捕食也是影响衰减的重要因素.基因工程菌在连续运行的膜生物反应器和活性污泥反应器中的细胞密度衰减,也可以用Logistic模型拟合,其参数在间歇实验的取值范围内.

关键词: 川鄂连蕊茶, 生态对策, 适合度, 生殖值

Abstract: With batch experiment and continuous-running bioreactor, this paper studied the decay of the population size of genetically engineered microorganisms (GEMs) in activated sludge. The decay models and their parameters were obtained, and several affecting factors were analyzed. The results showed that both in batch experiment and in continuous-running bioreactor, the population size of the GEMs decreased rapidly initially, and then tended to keep constant. The dynamics of decay could be fitted well by Logistic model and Gompertz model, and no difference was observed between the fit goodness of the models. Under different conditions, the value ranges of the parameters in batch experiment were decay coefficient r′=0.5—0.6 h-1 and stable population size K′=104—105 cfu·ml-1 for Logistic model, and initial decay coefficient b=0.6—1.2 h-1 and specific decay coefficient a=0.02—0.09 h-1 for Gompertz model. The initial population size had a little effect on the decay, but affected stable population size remarkably. Mixed liquor suspended solid (MLSS) and temperature both affected remarkably, i.e., the decay became faster but got larger when MLSS was smaller, whereas the decay became faster and got smaller when temperature was lower. Nutrient conditions and predation by protozoa and metozoa were also the important affecting factors of decay. In continuous-running membrane bioreactor and conventional activated sludge reactor, Logistic model could also fit the dynamics of decay well, and the values of parameters were in the ranges obtained from batch experiment.

Key words: Camellia rosthorniana, Bionomic strategies, Fitness, Reproductive value