南方红豆杉枝叶中药用抗癌活性物质含量

应用生态学报

南方红豆杉枝叶中药用抗癌活性物质含量

杨逢建;庞海河;张学科;孙佳音;祖元刚

东北林业大学森林植物生态学教育部重点实验室，哈尔滨 150040

收稿日期:2007-08-23 修回日期:1900-01-01 出版日期:2008-04-20 发布日期:2008-04-20

Quantitative changes of anticancer active components in Taxus chinensis var.mairei branches and leaves.

YANG Feng-jian; PANG Hai-he;ZHANG Xue-ke; SUN Jia-yin;ZU Yuan-gang

Key Laboratory of Forest Plant Ecology of Education Ministry, Northeast Forestry University, Harbin 150040, China

Received:2007-08-23 Revised:1900-01-01 Online:2008-04-20 Published:2008-04-20

摘要/Abstract

摘要： 通过高效液相色谱（HPLC），测定了人工栽培南方红豆杉枝叶中药用抗癌活性物质紫杉醇（taxol）、三尖杉宁碱（cephalomannine）和人工半合成紫杉醇的主要原料巴卡亭Ⅲ（baccatin Ⅲ）、10去乙酰基巴卡亭Ⅲ（10-deacetylbaccatin Ⅲ，10-DAB）在一个生长季的含量变化.研究表明：从4月新枝叶萌发至11月枝叶基本停止生长，南方红豆杉枝叶中紫杉醇等药用活性物质含量季节性变化明显.其中紫杉醇和三尖杉宁碱含量的最高值都出现在5月；巴卡亭Ⅲ和10-去乙酰基巴卡亭Ⅲ含量的最高峰值分别出现在9月和4月.相关分析发现，南方红豆杉枝叶中4种药用活性物质之间有很好的相关性，其中紫杉醇与三尖杉宁碱含量呈显著正相关(P<0.05)，三尖杉宁碱含量与10-去乙酰巴卡亭Ⅲ含量呈显著负相关(P<0.05).

Abstract: The quantitative changes of anticancer ingredients such as taxol, cephalomannine, baccatin Ⅲ and 10-deacetylbaccatin Ⅲ in the branches and leaves of Taxus chinensis var. maireiduring one growth season were measured by HPLC. The results showed that the contents of these anticancer ingredients had an obvious change from April when the new branches began to germinate to November when the branches basically stopped growing. The contents of taxol and cephalomannine in the branches and leaves of T. chinensis had the same change pattern, with the peaks appeared in May, and those of Baccatin Ⅲ and 10-deacetylbaccatin Ⅲ had the same change trend, with the highest values appeared in September and April, respectively. There was a significant positive correlation between the contents of taxol and cephalomannine (P<0.05), and a negative correlation between the contents of cephalomannine and 10-deacetylbaccatin Ⅲ (P<0.05).

杨逢建;庞海河;张学科;孙佳音;祖元刚. 南方红豆杉枝叶中药用抗癌活性物质含量[J]. 应用生态学报.

YANG Feng-jian; PANG Hai-he;ZHANG Xue-ke; SUN Jia-yin;ZU Yuan-gang . Quantitative changes of anticancer active components in Taxus chinensis var.mairei branches and leaves. [J]. Chinese Journal of Applied Ecology.