Abstract: Taking substrates swine manure and wheat straw (fresh mass ratio 10.5:1) as the control (PMW), a composting experiment was conducted in a selfmade aerated static composting bin to study the effects of adding 8% grape seed (treatment PMW+G) on the succession of microbial community and the transformation of carbon and nitrogen in the substrates during the composting. Seven samples were collected from each treatment, according to the temperature of the compost during the 30 d composting period. The microbial population and physiological groups were determined, and the NH₄⁺-N, NO₃^--N, organic N, and organic C concentrations in the compost were measured. Grape seed addition induced a slight increase of bacterial count and a significant increase of actinomycetes count, but decreased the fungal count significantly. Grape seed addition also decreased the ratio of bacteria to actinomycetes and the counts of ammonifiers and denitrifiers, but increased the counts of nitrifiers, Nfixing bacteria, and cellulosedecomposing microorganisms. The contents of NH₄⁺-N and organic C decreased, while that of NO₃^--N increased obviously. The NO₃^--N content in the compost was positively correlated with the actinomycetes count. During composting, the compost temperature in treatment PMW+G increased more rapidly, and remained steady in thermophilic phase, while the water content changed little, which provided a stable and higher population of actinomycetes and nitrifiers in thermophilic phase, being beneficial to the increase of compost nitrate N.