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水-水循环在生姜叶片耗散过剩光能中的作用

张永征,李海东,李秀,徐坤**   

  1. (山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点实验室, 山东泰安 271018)
  • 出版日期:2014-01-18 发布日期:2014-01-18

Role of water-water circulation in excessive light energy dissipation of ginger leaves.

ZHANG Yong-zheng, LI Hai-dong, LI Xiu, XU Kun   

  1. (College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, Ministry of Agriculture, Tai’an 271018, Shandong, China)
  • Online:2014-01-18 Published:2014-01-18

摘要:

为探讨水-水循环对生姜叶片的光保护作用,通过对生姜采取叶面喷施10 mmol·L-1碘乙酰胺(IA)与遮光50%交互处理,研究了自然强光+清水(T1)、遮光50%+清水(T2)、自然强光+10 mmol·L-1 IA(T3)、遮光50%+10 mmol·L-1 IA(T4)对生姜叶片叶绿素荧光参数、Mehler反应及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性的影响.结果表明: 处理过程中,T3、T4处理生姜叶片光合速率及最大光化学效率均持续降低,至处理9 d时,光合速率分别较处理前降低了64%和33.2%,最大光化学效率分别降低了16.5%和10.9%,Mehler反应分别升高了139.4%和72.6%,且SOD和APX活性显著增强,而T1、T2则无显著变化.处理6 d时,一天内各处理生姜叶片光合速率、最大光化学效率在午间均显著降低,叶片的Mehler反应及SOD、APX活性在午间均显著增强,但以T3变幅较大,T4次之,T1、T2较小.可见,外源IA抑制生姜叶片光合速率后,Mehler反应及活性氧清除酶活性增强,表明水-水循环在耗散生姜叶片过剩光能中起重要作用.
 

Abstract: To investigate the photoprotection of waterwater circulation in ginger leaves, the effects of different treatments such as natural light + water control (T1), shading 50% + water control (T2), natural light + 10 mmol·L-1 IA (T3), shading 50% + 10 mmol·L-1 IA (T4) on leaf chlorophyll fluorescence parameters, Mehler reaction and the activities of SOD, APX of potted ginger were studied. The results showed that the Pn and Fv/Fm of ginger leaves in T3 and T4 reduced constantly, but that of T1 and T2 had no significant change during treatment. For example, at the ninth day after treatment, the Pn of T3 and T4 decreased by 64% and 33.2% respectively, and the Fv/Fm decreased by 16.5% and 10.9% respectively, while Mehler reaction of T3 and T4 increased by 139.4% and 72.6% respectively, the activity of SOD and APX were significantly higher than those of the controls. At the sixth day after treatment, the leaf Pn and Fv/Fm in all treatments reduced significantly at noon, but Mehler reaction and the activities of SOD and APX increased markedly, and the largest amplitude was observed in T3, followed by T4, and then T1 and T2. So, it was clear that Mehler reaction and the activity of reactiveoxygen scavenging enzymes increased when leaf Pn was inhibited by exogenous IA. The results suggested that waterwater circulation played an essential role in dissipating excessive light energy of ginger leaves.