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笋秀夜蛾雄蛾对性信息素化合物的电生理和行为反应

张爱良1,周樟庭2,张亚波3,周智峰2,沈志连4,王浩杰3,舒金平3**   

  1. (1安吉县林业局, 浙江湖州 313300; 2遂昌县林业局, 浙江丽水 323300; 3中国林业科学研究院亚热带林业研究所, 浙江富阳 311400; 4武康镇人民政府, 浙江湖州 313300)
  • 出版日期:2014-10-18 发布日期:2014-10-18

Electrophysiological and behavioral responses of male Apamea apameoides (Draudt) (Lepidoptera: Noctuidae) to sex pheromone components.

ZHANG Ai-liang1, ZHOU Zhang-ting2, ZHANG Ya-bo3, ZHOU Zhi-feng2, SHEN Zhi-lian4, WANG Hao-jie3, SHU Jin-ping3   

  1. (1Anji Bureau of Forestry, Huzhou 313300, Zhejiang, China; 2Suichang Bureau of Forestry, Lishui 323300, Zhejiang, China; 3Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China; 4The People’s Government of Wukang Town, Huzhou 313300, Zhejiang, China)
  • Online:2014-10-18 Published:2014-10-18

摘要: 利用气质联用仪对笋秀夜蛾雌蛾性腺体粗提物成分进行了分析比对,通过触角电位(EAG)试验研究了笋秀夜蛾雄成虫对性信息素组分的电生理反应,以Y形嗅觉仪进行了室内和林间的行为生测.结果表明: 雌蛾性腺体粗提物中顺11-十六碳乙酸酯(Z11-16:Ac)和顺11-十六碳烯醇(Z11-16:OH)为笋秀夜蛾性信息素活性组分,性腺粗提物、两种化合物及其混合物均能激发雄蛾明显的EAG反应,且EAG反应值随性信息素组分浓度增加而增加.Z11-16:Ac和Z11-16:OH按57∶43配置成的二元混合物所激发的EAG反应强于同等剂量下的单一组分.室内的行为生测和林间诱捕试验结果与EAG反应基本一致,Z11-16:Ac+Z11-16:OH(57∶43)混合物对雄蛾引诱力最强,在林间单诱捕器(诱芯浓度为104ng·μL-1)的平均诱捕量达到(48.5±6.7)头.

Abstract: The sex pheromone gland extracts collected from calling females of Apamea apameoides (Lepidoptera: Noctuidae) were analyzed with GC-MS, the electrophysiological and behavioral responses of the male adults to serial dilutions of sex pheromone components and their synthetic blends were investigated with Ytube olfactometer in laboratory and in bamboo forest field. The results indicated that (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol were the functional components in the sex pheromone gland extracts. Electroantennogram (EAG) recordings showed that sex pheromone gland extracts, (Z)-11-hexadecenyl acetate, (Z)-11-hexadecen-1-ol and the mixture of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol all could elicit strong EAG responses, and the average EAG values increased with the increasing concentration of the sex pheromone. The blends of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol at the ratio of 57:43 elicited a higher EAG value than each singular component did. The results of behavioral assay by Y-tube olfactometer accorded with those of EAG responses on the whole, and the mixture of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol at the ratio of 57:43 was more attractive than each component alone. In field tests with silicone rubber as pheromone dispensers (concentration=104 ng·uL-1), the average number of male adults captured per trap by the mixture was (48.5±6.7).