硫酸黏菌素残留对土壤微生物群落结构特征的影响

应用生态学报

硫酸黏菌素残留对土壤微生物群落结构特征的影响

马驿¹,彭金菊¹,陈进军¹,范葶莉¹,孙永学^2**

(¹广东海洋大学农学院动物医学系, 广东湛江 524088; ^2华南农业大学广东省兽药研制与安全评价重点实验室, 广州 510642)

出版日期:2014-06-18 发布日期:2014-06-18

Effects of colistin sulfate residue on soil microbial community structure.

MA Yi¹, PENG Jin-ju¹, CHEN Jin-jun¹, FAN Ting-li¹, SUN Yong-xue^2

(¹Department of Veterinary Medicine, College of Agricultural, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China; ²Guangdong Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China)

Online:2014-06-18 Published:2014-06-18

摘要/Abstract

摘要：

采用氯仿熏蒸法和磷脂脂肪酸(phospholipid fatty acid,PLFA)方法,分析了硫酸黏菌素(colistin sulfate,CS)残留后土壤微生物群落结构的变化.结果表明: 硫酸黏菌素(w_CS≥5 mg·kg^-1)对土壤微生物生物量碳影响显著,土壤中硫酸黏菌素浓度愈高,微生物生物量碳愈低,50 mg·kg^-1的硫酸黏菌素处理使土壤微生物生物量碳下降52.1%.在整个采样周期中,每克土壤总PLFA含量在硫酸黏菌素胁迫下出现明显降低,且存在一定的剂量依赖效应.第7、49天时,低浓度组(w_CS=0.5 mg·kg^-1)和对照组分异不明显,第21、35天时,处理间土壤微生物群落结构多样性类型分异显著,其中高浓度组(w_CS=50 mg·kg^-1)与对照组分异最大.表明硫酸黏菌素可致土壤微生物群落结构多样性改变,并表现出时间差异.这可能与硫酸黏菌素在土壤中化学结构发生了改变和降解有关.

Abstract: By using fumigation extraction and phospholipid fatty acid (PLFA) methods, the change of characteristics of soil microbial community structure caused by residue of colistin sulfate (CS) was studied. The results showed that the CS (w_CS≥5 mg·kg^-1) had a significant effect on the microbial biomass carbon (MBC) and it was dosedependent where MBC decreased with the increase of CS concentration in soil. The MBC in soil decreased by 52.1% when the CS concentration reached 50 mg·kg^-1. The total PLFA of soil in each CS treatment was significantly decreased during the sampling period compared with the control group and showed a dosedependent relationship. The soil microbial community structure and diversity in the low CS group (w_CS=0.5 mg·kg^-1) were not significantly different from the control group on 7th and 49th day. However, they were significantly different on 21st and 35th day especially in the high CS group (w_CS=50 mg·kg^-1). It was concluded that CS could change the structure of soil microorganisms and varied with time which might be caused by the chemical conversion and degradation of CS in soil.

马驿1,彭金菊1,陈进军1,范葶莉1,孙永学2**. 硫酸黏菌素残留对土壤微生物群落结构特征的影响[J]. 应用生态学报.

MA Yi1, PENG Jin-ju1, CHEN Jin-jun1, FAN Ting-li1, SUN Yong-xue2. Effects of colistin sulfate residue on soil microbial community structure.[J]. Chinese Journal of Applied Ecology.