添加葡萄糖和淀粉对盆栽甜樱桃根区土壤碳代谢及根功能的影响

应用生态学报

添加葡萄糖和淀粉对盆栽甜樱桃根区土壤碳代谢及根功能的影响

周文杰^1,2,张鹏^1,2,秦嗣军^1,2**,吕德国^1,2

(¹沈阳农业大学园艺学院, 沈阳 110866; ²辽宁省果树品质发育与调控重点实验室, 沈阳 110866)

出版日期:2015-11-18 发布日期:2015-11-18

Effects of exogenous glucose and starch on soil carbon metabolism of root zone and root function in potted sweet cherry.

ZHOU Wen-jie^1,2, ZHANG Peng^1,2, QIN Si-jun^1,2, LYU De-guo^1,2

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China; ²Liaoning Province Key Laborary of Fruit Quality Development and Regulation, Shenyang 110866, China)

Online:2015-11-18 Published:2015-11-18

摘要/Abstract

摘要： 在1年生盆栽甜樱桃土壤中添加葡萄糖和淀粉(4 g·kg^-1)，以不添加外源碳为对照，处理后0~60 d内定期采根区土样测定土壤微生物生物量碳、蔗糖酶和淀粉酶活性以及微生物群落功能多样性，处理后第30天测定根系呼吸速率、呼吸途径和根系活力.结果表明：添加葡萄糖后，土壤蔗糖酶活性及微生物生物量碳均表现为先升高再降低，峰值分别出现在处理后第15天及第7天，分别高于对照14.0%和13.1%，土壤有机质含量表现为先升高再降低再缓慢回升；添加淀粉后显著提高了土壤淀粉酶活性，第15天时为对照的8.5倍，土壤微生物生物量碳除在第7天低于对照外，其余时期均高于对照，土壤有机质含量表现为先升高再下降，处理后第60天高于对照19.8%.BIOLOG分析表明，处理后第15天平均吸光度（AWCD）值及微生物活性均达到最大值，表现为淀粉>葡萄糖>对照.处理后第30天，葡萄糖处理显著增加了土壤微生物对碳水化合物类、羧酸类、氨基酸类、酚酸类和胺类碳源的利用，淀粉处理显著增加了土壤微生物对碳水化合物类、羧酸类、聚合物类和酚酸类碳源的利用.处理后第30天，葡萄糖处理甜樱桃根系总呼吸速率分别较对照及淀粉处理提高21.4%和19.4%，根系活力分别提高65.5%和37.0%.添加葡萄糖和淀粉影响了甜樱桃根区土壤稳定碳源及不稳定碳源的代谢过程，整体上提高了土壤微生物活性，增强了甜樱桃根系呼吸速率及根系活力.

Abstract: One-year-old potted sweet cheery trees were treated with 4 g·kg^-1 exogenous glucose or starch and with nonaddition of exogenous carbon as the control for up to 60 days. Soil of root zone was sampled to analyze soil microbial biomass carbon, activities of invertase and amylase and microbial community functional diversity during the 60day treatment, and roots were sampled for analysis of root respiratory rate, respiratory pathways and root viability after treatment for 30 days. Results showed that the invertase activity and the microbial biomass carbon initially increased and decreased subsequently, with the maxima which were 14.0% and 13.1% higher in the glucose treatment than in the control treatment appeared after 15 and 7 days of treatments, respectively. Soil organic matter content increased first then decreased and finally moderately increased again. Amylase activity was 7.5fold higher in the starch treatment than in the control treatment after 15-day treatment. Soil microbial biomass carbon was higher in the starch treatment than in the control treatment except after 7-day treatment. Soil organic matter content initially increased and then decreased, but it was still 19.8% higher than in the control after 60day treatment. BIOLOG results showed that the maximum average well color development (AWCD) value and microbial activity appeared after 15-day treatment in the following order: starch>glucose>control. After 30-day treatment, glucose treatment resulted in a significant increase in the soil microbial utilization of carbohydrates, carboxylic acid, amino acids, phenolic acids and amines, and starch treatment significantly increased the soil microbial utilization of carbohydrates, carboxylic acid, polymers and phenolic acids. After 30-day treatment, the total root respiratory rate and root viability were 21.4%, 19.4% and 65.5%, 37.0% higher in glucose treatment than in the control and starch treatments, respectively. These results indicated exogenous glucose and starch affected soil carbon metabolism and enhanced soil microbial activity, the root respiratory rate and root viability.

周文杰1,2,张鹏1,2,秦嗣军1,2**,吕德国1,2. 添加葡萄糖和淀粉对盆栽甜樱桃根区土壤碳代谢及根功能的影响[J]. 应用生态学报.

ZHOU Wen-jie1,2, ZHANG Peng1,2, QIN Si-jun1,2, LYU De-guo1,2. Effects of exogenous glucose and starch on soil carbon metabolism of root zone and root function in potted sweet cherry.[J]. Chinese Journal of Applied Ecology.