Abstract: Soil samples were collected from an alpine coniferous forest. Soil cores with soil organic layer above and mineral soil layer below were incubated in plant growth chambers during 10 weeks. Taking the annual average soil temperature in the alpine forest as the control, and other two levels of temperature were increased 2 and 4 ℃, respectively, to investigate the responses of soil microbial community and soil enzyme activity to warming. The results showed that warming significantly reduced PLFAs content of bacteria in soil organic layer and PLFAs content of G^- in the mineral soil layer, whereas soil fungi was slightly influenced by warming. Warming caused the G⁺/G^- increasing and changed the microbial community structure, but had no significant effect on soil enzymes activity, i.e., laccase (Lac), β-1,4-glucoside (BG), acid phosphate (AP), β-1,4-N-acetylglucosaminidase (NAG). There were significantly positive correlations between fungi, bacteria, G⁺ and G^-, showing the coordinated growth trend between soil microbial communities. There was significantly negative correlation between BG and bacteria, showing the BG competing for carbon source utilization with soil microbial community. In consequence, the soil microbe types responded differently to warming. Bacteria were more sensitive to temperature than fungi, and fungi had stronger ability to tolerate warming in the alpine forest.