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应用生态学报 ›› 2016, Vol. 27 ›› Issue (9): 3009-3015.doi: 10.13287/j.1001-9332.201609.020

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水杨酸和油菜素内酯对低温胁迫下黄瓜幼苗光合作用的影响

徐晓昀, 郁继华*, 颉建明, 胡琳莉, 李杰   

  1. 甘肃农业大学园艺学院, 兰州 730070
  • 收稿日期:2016-01-29 发布日期:2016-09-18
  • 通讯作者: * E-mail: yujihua@gsau.edu.cn
  • 作者简介:徐晓昀,男,1981年生,博士研究生. 主要从事设施蔬菜栽培研究. E-mail: xuxy@gsau.edu.cn
  • 基金资助:
    甘肃农业大学盛彤笙基金项目(GSAU-STS-1434)和国家自然科学基金项目(31260473)资助

Effects of exogenous salicylic acid and brassinolide on photosynthesis of cucumber seedlings under low temperature stress.

XU Xiao-yun, YU Ji-hua*, XIE Jian-ming, HU Lin-li, LI Jie   

  1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2016-01-29 Published:2016-09-18
  • Contact: * E-mail: yujihua@gsau.edu.cn
  • Supported by:
    This work was supported by the SHENG Tong-sheng Foundation of Gansu Agricultural University (GSAU-STS-1434) and National Natural Science Foundation of China (31260473)

摘要: 为了探明外源水杨酸(SA)和2,4-表油菜素内酯(EBR)对低温胁迫下黄瓜幼苗光合作用的调控机理,以‘优博1-5’黄瓜为试材,用1 mmol·L-1SA和0.1 μmol·L-1EBR喷施预处理幼苗,每天喷1次,连喷2 d后置于低温下[10 ℃/5 ℃,光强(PFD)80 μmol·m-2·s-1]处理.结果表明: 低温胁迫下黄瓜幼苗生长量及净光合速率(Pn)下降;喷施SA和BER显著提高了Pn、光系统Ⅱ最大光化学效率(Fv/Fm)、光系统Ⅱ实际光化学效率(ΦPS)和光化学猝灭系数(qP),减缓了非光化学猝灭系数(NPQ)增加的幅度,同时核酮糖-1,5-二磷酸羧化/加氧酶(Rubisco)、景天庚酮糖-1,7-二磷酸酯酶(SBPase)、转酮醇酶(TK)和果糖-1,6-二磷酸醛缩酶(FBA)活性明显升高.说明SA和EBR可以通过调节光合关键酶的活性,缓解低温对黄瓜幼苗光合作用的影响,增强其对低温的适应性.

Abstract: An experiment was conducted to investigate the mechanism of the regulation of salicylic acid (SA) and 2,4-epibrassionolide (EBR) on photosynthesis in cucumber (Cucumis sativa) seedlings under low temperature stress. Taking cucumber cultivar ‘Youbo1-5’ as material, the seedlings were pre-treated with 1 mmol·L-1 SA or 0.1 μmol·L-1EBR (sprayed once a day), and then were exposed to chilling temperature (10 ℃/5 ℃, PFD 80 μmol·m-2·s-1) after being pre-treated 2 days. The results showed that the growth and net photosynthetic rate (Pn) of cucumber seedlings were decreased under low temperature stress. However, the Pn, maximal photochemical efficiency of PSⅡ (Fv/Fm), actual photochemical efficiency of PSⅡ (ΦPS) and photochemical quenching coefficient (qP) were significantly improved in SA- and EBR-pretreated seedlings, and the increase range of non-photochemical quenching coefficient (NPQ) was decreased. Moreover, the activities of ribulose 1,5-biphosphate carboxylase/oxygenase (Rubisco), sedoheptulose-1,7-bisphosphatase (SBPase), fructose-1,6-bisphosphate aldolase (FBA) and transketolase (TK) were signi-ficantly increased. These findings suggested that SA and EBR improved photosynthesis of cucumber seedlings by promoting the activities of key enzymes and increased low temperature tolerance.