干旱下接种根际促生细菌对苹果实生苗光合和生理生态特性的影响

doi:10.13287/j.1001-9332.201910.024

应用生态学报 ›› 2019, Vol. 30 ›› Issue (10): 3501-3508.doi: 10.13287/j.1001-9332.201910.024

干旱下接种根际促生细菌对苹果实生苗光合和生理生态特性的影响

徐雪东¹, 张超², 秦成¹, 苏芸芸¹, 周洁³, 张海², 张立新^1*

¹西北农林科技大学生命科学学院, 陕西杨凌 712100;
²西北农林科技大学资源环境学院, 陕西杨凌 712100;
³西北农林科技大学化学与药学院, 陕西杨凌 712100

收稿日期:2018-11-09 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: *E-mail: zhanglixin@nwsuaf.edu.cn
作者简介:徐雪东, 男, 1992年生, 硕士研究生. 主要从事植物逆境水肥研究. E-mail: 15038019948@163.com
基金资助:
国家重点研发计划项目(2017YFE0114000)、陕西省水利科技计划项目(2015slkj-12)和农业农村部公益性行业科研专项(201303104)资助

Effects of PGPR inoculation on photosynthesis and physiological-ecological characteristics of apple seedlings under drought stress

XU Xue-dong¹, ZHANG Chao², QIN Cheng¹, SU Yun-yun¹, ZHOU Jie³, ZHANG Hai², ZHANG Li-xin^1*

¹College of Life Sciences, Northwest A＆F University, Yangling 712100, Shaanxi, China;²College of Natural Resources and Environment, Northwest A＆F University, Yangling 712100, Shaanxi, China;
³College of Chemistry ＆ Pharmacy, Northwest A＆F University, Yangling 712100, Shaanxi, China

Received:2018-11-09 Online:2019-10-20 Published:2019-10-20
Contact: *E-mail: zhanglixin@nwsuaf.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2017YFE0114000), the Water Resources Science and Technology Project of Shaanxi Province (2015slkj-12) and the Special Fund for Scientific Research in the Public Interest of Ministry of Agricuture and Rural Affairs (201303104).

摘要/Abstract

摘要： 本试验研究了接种根际促生细菌(PGPR)对干旱条件下植物光合和生理生态特性的影响,以期为PGPR在植物抗旱中的应用提供理论依据.采用盆栽试验,以苹果实生幼苗为供试植物,以经过筛选得到的既具有1-氨基环丙烷-1-羧酸(ACC)脱氨酶活性又具有较强溶磷能力的根际促生菌YX₂为供试菌株,设置正常水分(CK)、轻度干旱(LD)、中度干旱(MD)和重度干旱(SD),其相应含水量分别为田间持水量的70%～80%、55%～65%、40%～50%、25%～35%,研究不同程度干旱胁迫条件下接种YX₂对苹果实生幼苗光合和生理生态特性的影响.结果表明: 与未接种处理相比,干旱环境下接种YX₂提高了苹果幼苗叶片的相对含水量、叶绿素含量、抗氧化酶活性、叶绿素荧光值、气孔导度和光合性能,降低了相对电导率、渗透调节物质和丙二醛的积累,缓解了干旱胁迫对净光合速率的抑制,增强了抗氧化系统的防御能力,减少了细胞膜过氧化伤害,提高了植株抗旱性能.

Abstract: The effects of inoculation of rhizosphere-promoting bacteria (PGPR) on photosynthesis and physiological-ecological characteristics of apple tree seedlings under drought conditions were investigated in this study, aiming to provide a theoretical basis for the application of PGPR in plant drought resistance. In the pot experiment, the rhizosphere-promoting bacterium YX₂ which had both ACC deaminase activity and strong phosphorus solubilizing ability was selected as the tested strain. Apple seedlings were grown under four different irrigation levels i.e., control (CK), mild drought (LD), moderate drought (MD), and severe drought (SD) with soil moisture equivalent to 70%-80%, 55%-65%, 40%-50% and 25%-35% of field water holding capacity, respectively. Inoculation of PGPR alleviated the damaging effects of drought on growth by improving relative water content and chlorophyll content in apple tree seedlings. In addition, PGPR inoculated individuals exhibited higher antioxidant enzyme activity, chlorophyll fluorescence values, stomatal conductance and photosynthetic rate and lower relative conductivity and lipid peroxidation. Our results suggested that PGPR-YX₂ alleviated the negative effects of drought stress on the growth and net photosynthetic rate by improving the antioxidant system, water content and membrane functioning.

徐雪东, 张超, 秦成, 苏芸芸, 周洁, 张海, 张立新. 干旱下接种根际促生细菌对苹果实生苗光合和生理生态特性的影响[J]. 应用生态学报, 2019, 30(10): 3501-3508.

XU Xue-dong, ZHANG Chao, QIN Cheng, SU Yun-yun, ZHOU Jie, ZHANG Hai, ZHANG Li-xin. Effects of PGPR inoculation on photosynthesis and physiological-ecological characteristics of apple seedlings under drought stress[J]. Chinese Journal of Applied Ecology, 2019, 30(10): 3501-3508.

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干旱下接种根际促生细菌对苹果实生苗光合和生理生态特性的影响

Effects of PGPR inoculation on photosynthesis and physiological-ecological characteristics of apple seedlings under drought stress

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