芒草种植对土壤细菌群落结构和功能的影响

doi:10.13287/j.1001-9332.201906.038

应用生态学报 ›› 2019, Vol. 30 ›› Issue (6): 2030-2038.doi: 10.13287/j.1001-9332.201906.038

芒草种植对土壤细菌群落结构和功能的影响

段鹏飞¹, 陈彦², 张菲², 韩辉¹, 庞发虎¹, 陈兆进^1,*, 田伟³

¹南阳师范学院农业工程学院/南水北调中线水源区水安全河南省协同创新中心, 河南南阳 473061;
²南阳师范学院生命科学与技术学院, 河南南阳 473061;
³环境保护部南京环境科学研究所, 南京 210042

收稿日期:2018-08-16 出版日期:2019-06-15 发布日期:2019-06-15
通讯作者: * E-mail: zhaojin_chen@163.com
作者简介:段鹏飞,男,1983年出生,博士,讲师.主要从事作物生理生态研究. E-mail: dpf19830905@126.com
基金资助:
国家自然科学基金项目(41601332,31501260)、河南省科技攻关计划项目(172102110259,182102110468,182102110239)和河南省教育厅高等学校重点科研项目(16A210012)资助

Effect of Miscanthus planting on the structure and function of soil bacterial community.

DUAN Peng-fei¹, CHEN Yan², ZHANG Fei², HAN Hui¹, PANG Fa-hu¹, CHEN Zhao-jin^1,*, TIAN Wei³

¹School of Agricultural Engineering, Nanyang Normal University/Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Nanyang 473061, Henan, China;
²School of Life Science and Technology, Nanyang Normal University,Nanyang 473061, Henan, China;
³Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China

Received:2018-08-16 Online:2019-06-15 Published:2019-06-15
Supported by:
This work was supported by National Natural Science Foundation of China (41601332, 31501260), the Key Science and Technology Project of Henan Province (172102110259, 182102110468, 182102110239), and the Key Research Project of Colleges and Universities of Henan Province Education Department (16A210012)

摘要/Abstract

摘要： 芒草作为第二代能源植物的代表,其生长过程中根际土壤细菌群落的结构与功能尚不清楚.本研究以种植5年的芒草(湘杂芒1号)为研究对象,选取裸地作为对照,采用16S rRNA基因Miseq测序技术研究其细菌群落组成,同时采用PICRUSt功能预测分析其功能.结果表明: 芒草根际细菌由变形菌门、酸杆菌门、放线菌门、绿弯菌门、拟杆菌门和芽单胞菌门等23个门、231个属的细菌组成,表现出群落组成的丰富性.细菌群落分析表明,种植湘杂芒1号改变了根际细菌群落结构,其细菌群落多样性低于裸地对照.PICRUSt功能预测分析表明,湘杂芒1号根际细菌主要涉及氨基酸运输和代谢、细胞壁/细胞膜/膜结构的生物合成、信号转导机制等24个基因功能家族,表现出功能上的丰富性,并有22个基因功能家族预测基因相对丰度高于裸地,表明种植湘杂芒1号提高了根际细菌功能.对氮、磷循环相关基因进行分析表明,种植湘杂芒1号改变了土壤氮、磷代谢能力.

Abstract: Miscanthus is a promising candidate species of second-generation energy crops. To our knowledge, the composition and function of Miscanthus rhizosphere bacterial communities has not yet been reported. In this study, rhizosphere soil samples were collected from Miscanthus (Xiangzamang No. 1) which was grown in Nanyang City for five years and bareground (as the control) to analyze the bacterial community structure and diversity using 16S rRNA gene sequencing on the Illumina MiSeq platform. PICRUSt was used to determine the metabolic and functional abilities of soil bacterial communities. Phylogenetic analysis based on 16S rRNA gene sequences showed that sol bacteria could be divided into 23 phyla and 231 genera, with high richness of the community composition. The dominant phyla were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Bacteroidetes and Gemmatimonadetes. The bacterial community diversity in the rhizosphere samples of Xiangzamang No. 1 was lower than that in unplanted samples. Rhizosphere bacterial communities were affected by Miscanthus crops. Based on the PICRUSt analysis of bacterial community functions, our results revealed a wide genetic diversity of organisms involved in various essential processes,such as amino acid transport and metabolism, cell wall/membrane/envelope biogenesis, signal transduction mechanisms. Based on the 16S rRNA gene copy number of the detected phylotypes, 22 bacterial metabolic function in the rhizosphere samples of Miscanthus was higher than that in bareground. Results from N- and P-cycling gene analysis showed that Miscanthus planting altered the N- and P-cycling metabolic capacities of soil bacteria.

段鹏飞, 陈彦, 张菲, 韩辉, 庞发虎, 陈兆进, 田伟. 芒草种植对土壤细菌群落结构和功能的影响[J]. 应用生态学报, 2019, 30(6): 2030-2038.

DUAN Peng-fei, CHEN Yan, ZHANG Fei, HAN Hui, PANG Fa-hu, CHEN Zhao-jin, TIAN Wei. Effect of Miscanthus planting on the structure and function of soil bacterial community.[J]. Chinese Journal of Applied Ecology, 2019, 30(6): 2030-2038.

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芒草种植对土壤细菌群落结构和功能的影响

Effect of Miscanthus planting on the structure and function of soil bacterial community.

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