施加内生真菌对花生连作土壤微生物和酶活性的影响

应用生态学报 ›› 2012, Vol. 23 ›› Issue (10): 2693-2700.

施加内生真菌对花生连作土壤微生物和酶活性的影响

王宏伟¹,王兴祥^2,3，4,吕立新¹,肖逸¹,戴传超^1**

(¹南京师范大学生命科学学院江苏省微生物与功能基因组学重点实验室，南京 210046； ²中国科学院土壤环境与污染修复重点实验室，南京 210008； ³中国科学院南京土壤研究所，南京 210008；⁴国家红壤改良工程技术研究中心，江西鹰谭， 335211)

出版日期:2012-10-18 发布日期:2012-10-18

Effects of applying endophytic fungi on the soil biological characteristics and enzyme activities under continuously cropped peanut.

WANG Hong-wei¹, WANG Xing-xiang^2，3，4, LU Li-xin¹, XIAO Yi¹, DAI Chuan-chao¹

(¹Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; ²Key Laboratory of Soil Environment and Pollution Remediation, Chinese Academy of Sciences, Nanjing 210008, China; ³Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; ^4National Engineering and Technology Research Center for Red Soil Improvement, Yingtan 335211, Jiangxi, China)

Online:2012-10-18 Published:2012-10-18

摘要/Abstract

摘要： 通过盆栽试验，研究了在土壤中施加植物内生真菌拟茎点霉NJ4.1菌株、B3菌株和角担子菌B6菌株在花生不同生育时期对土壤微生物学特性及酶活力的影响.结果表明： B3处理显著提高花生荚果产量，为对照的1.2倍；施加内生菌NJ4.1、B3和B6能显著增加花生根瘤数量，分别为对照的12、1.3和1.3倍.3个加菌处理全生育期平均细菌和放线菌数量高于对照，萌发期和苗期土壤微生物生物量碳(SMBC)显著高于对照，微生物生物量氮(SMBN)在花生萌发期升高，开花期降低.花生开花期土壤DGGE图谱表明，B3处理土壤细菌和真菌条带数量以及多样性最高.从萌发期到成熟期，与对照相比，3个加菌处理的土壤蔗糖酶和过氧化氢酶活性提高，脲酶活性无显著变化.表明施加内生菌对连作花生土壤环境有一定的改善作用，其中施用B3效果最好.

Abstract: A pot experiment was conducted to investigate the effects of applying endophytic fungi Phomopsis liquidambari strain B3, Phomopsis sp. strain NJ4.1, and Ceratobasidum stevensii strain B6 on the soil biological characteristics and enzyme activities under continuously cropped peanut at its different growth stages. Compared with the control, applying B3 increased the peanut yield significantly by 19.8%, and applying NJ4.1, B3 and B6 increased the peanut nodule number significantly by 20.4%, 29.3% and 27.6%, respectively. In the three treatments of applying endophytic fungi, the average population of soil bacteria and actinomycetes in the whole growth period of peanut was higher than that of the control, and the soil microbial biomass carbon was significantly greater at germination and seedling stages. The soil microbial biomass nitrogen increased at germination stage, but decreased at flowering stage. The DGGE analysis indicated that at flowering stage, the soil bacteria and fungi in treatment B3 had the largest band number and diversity. From germination stage to maturing stage, the three treatments of applying endophytic fungi had higher activities of soil invertase and catalase than the control, but less difference in soil urease activity. It was suggested that applying endophytic fungi could improve the peanut continuous cropping soil environment, and applying B3 had the best effect.

王宏伟1,王兴祥2,3，4,吕立新1,肖逸1,戴传超1**. 施加内生真菌对花生连作土壤微生物和酶活性的影响[J]. 应用生态学报, 2012, 23(10): 2693-2700.

WANG Hong-wei1, WANG Xing-xiang2，3，4, LU Li-xin1, XIAO Yi1, DAI Chuan-chao1. Effects of applying endophytic fungi on the soil biological characteristics and enzyme activities under continuously cropped peanut.[J]. Chinese Journal of Applied Ecology, 2012, 23(10): 2693-2700.