不同粒径红壤胶体颗粒对DNA的吸附特性

应用生态学报

不同粒径红壤胶体颗粒对DNA的吸附特性

廖敏^1,2,谢晓梅^1,2**,方舒^1,2,裘晓白^1,2,陈娜^1,2,徐雅倩^1,2,姜春燕³,陈雪芳³

(¹浙江大学环境与资源学院, 杭州 310058； ²浙江省亚热带土壤与植物营养重点实验室, 杭州 310058； ^3浙江省海盐县环境监测站, 浙江海盐 314300)

出版日期:2013-03-18 发布日期:2013-03-18

Characteristics of DNA adsorption on different sizes red soil colloidal particles.

LIAO Min^1,2, XIE Xiao-mei^1,2, FANG Shu^1,2, QIU Xiao-bai^1,2, CHEN Na^1,2, XU Ya-qian^1,2, JIANG Chun-yan³, CHEN Xue-fang³

(¹College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China; ²Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, Hangzhou 310058, China; ³ Haiyan Station of Environmental Monitoring, Haiyan 314300, Zhejiang, China)

Online:2013-03-18 Published:2013-03-18

摘要/Abstract

摘要：

采用平衡法研究了含有机质粗粘粒、去有机质粗粘粒、含有机质细粘粒和去有机质细粘粒4种红壤胶粒对DNA的吸附特征及其热力学特性.结果表明: 4种红壤胶粒对DNA的吸附是快反应过程,Langmuir吸附方程可较好地描述4种红壤胶体对DNA的等温吸附,相应拟合的相关系数r²分别为0.974、0.991、0.958和0.975.最大吸附量表现为含有机质细粘粒>去有机质细粘粒>含有机质粗粘粒>去有机质粗粘粒.电解质浓度和种类及吸附体系pH是影响红壤胶体对DNA吸附的重要因子,一定电解质浓度范围(NaCl<60 mmol·L^-1,CaCl₂<10 mmol·L^-1)内,DNA在红壤胶体表面的吸附量随电解质浓度的增大而显著增加,其中钙离子的促进作用大于钠离子,但随着吸附体系pH的上升而显著降低.含有机质胶粒对DNA的吸附过程是吸热反应,而去有机质胶粒对DNA的吸附过程是放热反应,红壤胶粒对DNA的吸附反应过程是一个熵增过程.

Abstract: By using balance reaction method, this paper studied the adsorption characteristics and thermodynamic properties of DNA on four kinds of red soil colloids (organic matter-contained coarse clay, organic matterremoved coarse clay, organic matter-contained fine clay, and organic matter-removed fine clay). The DNA adsorption on the four red soil colloids was a process of fast reaction, and the adsorption isotherms were conformed to the Langmuir equation, with the corresponding correlation coefficient (r²) being 0.974, 0.991, 0.958, and 0.975, respectively. The maximum adsorption amount of DNA on the colloidal particles followed the order of organic mattercontained fine clay > organic matterremoved fine clay ＞ organic matter-contained coarse clay ＞ organic matterremoved coarse clay, implying that the size and organic matter content of colloidal particles played an important role in DNA adsorption. Electrolyte concentration and type and adsorption system pH were the main factors affecting the DNA adsorption on the four soil colloids. Within a definite electrolyte concentration range (NaCl < 60 mmol·L^-1 and CaCl2 <10 mmol·L^-1), the adsorption amount of DNA on the red soil colloids increased significantly with the increase of electrolyte concentration. As compared with sodium ion, calcium ion had a greater promotion effect on the DNA adsorption, but the effect decreased significantly with the increase of adsorption system pH. The DNA adsorption on the organic matter-contained red soil colloids was an endothermic reaction, while the DNA adsorption on the organic matter-removed red soil colloids was an exothermic reaction. The DNA adsorption on the red soil colloids was a process of entropy increase.

廖敏1,2,谢晓梅1,2**,方舒1,2,裘晓白1,2,陈娜1,2,徐雅倩1,2,姜春燕3,陈雪芳3. 不同粒径红壤胶体颗粒对DNA的吸附特性[J]. 应用生态学报.

LIAO Min1,2, XIE Xiao-mei1,2, FANG Shu1,2, QIU Xiao-bai1,2, CHEN Na1,2, XU Ya-qian1,2, JIANG Chun-yan3, CHEN Xue-fang3. Characteristics of DNA adsorption on different sizes red soil colloidal particles.[J]. Chinese Journal of Applied Ecology.