• 综合评述 •
WENG You-zhu, FANG Yong-qiang, ZHANG Yu-sheng
Abstract: Lysosome is an important organelle existing in eukaryotic cells. With the development of the study on the structure and function of lysosome in recent years, lysosome is considered as a target of toxic substances on subcellular level, and has been widely applied abroad in marine pollution monitoring. This paper summarized the biological characteristics of lysosomal marker enzyme, lysosomeautophagy system, and lysosomal membrane, and introduced the principles and methods of applying lysosomal detection in marine pollution monitoring. Bivalve shellfish digestive gland and fish liver are the most sensitive organs for lysosomal detection. By adopting the lysosomal detection techniques such as lysosomal membrane stability (LMS) test, neutral red retention time (NRRT) assay, morphological measurement (MM) of lysosome, immunohistochemical (Ih) assay of lysosomal marker enzyme, and electron microscopy (EM), the status of marine pollution can be evaluated. It was suggested that the lysosome could be used as a biomarker for monitoring marine environmental pollution. The advantages and disadvantages of lysosomal detection and some problems worthy of attention were analyzed, and the application prospects of lysosomal detection were discussed.
翁幼竹,方永强,张玉生**. 溶酶体检测在海洋污染监测中的应用研究进展[J]. 应用生态学报.
WENG You-zhu, FANG Yong-qiang, ZHANG Yu-sheng. Application of lysosomal detection in marine pollution monitoring: Research progress.[J]. Chinese Journal of Applied Ecology.