施肥对设施菜地nirK型反硝化细菌群落结构和丰度的影响

应用生态学报

施肥对设施菜地nirK型反硝化细菌群落结构和丰度的影响

曾希柏^1**,王亚男¹,王玉忠²,白玲玉¹,李莲芳¹,段然¹,苏世鸣¹,吴翠霞¹

(¹中国农业科学院农业环境与可持续发展研究所/农业部农业环境重点实验室，北京 100081； ²甘肃省武威市凉州区农业技术推广中心，甘肃武威 733000)

出版日期:2014-02-18 发布日期:2014-02-18

Effects of different fertilization regimes on abundance and community structure of the nirKtype denitrifying bacteria in greenhouse vegetable soils.

ZENG Xi-bai¹, WANG Ya-nan¹, WANG Yu-zhong², BAI Ling-yu¹, LI Lian-fang¹, DUAN Ran¹, SU Shi-ming¹, WU Cui-xia¹

(¹Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Agricultural Environment and Climate Change, Beijing 100081, China; ²Liangzhou Center of Agricultural Technology Extension, Wuwei 733000, Gansu, China)

Online:2014-02-18 Published:2014-02-18

摘要/Abstract

摘要：

采用末端限制性片段多态性分析(T-RFLP)和实时荧光定量PCR(real-time PCR)方法，研究了甘肃武威设施菜地不同施肥条件下0～20 cm、20～40 cm土层中土壤nirK型反硝化细菌群落结构和丰度的变化.结果表明：施肥对土壤中nirK型反硝化细菌的群落结构具有明显影响，且对70、156、190 bp片段所代表设施菜地土壤优势种群影响最显著.施肥对0～20 cm土层nirK型反硝化细菌丰度有明显影响，其最大值出现在全有机肥(M)处理、为每克干土2.16×10⁷个拷贝数，分别是对照(CK)和全化肥(NPK)处理的2.04和2.02倍.设施菜地土壤0～20 cm与20～40 cm土层nirK型反硝化细菌的优势种群及其基因丰度均存在显著差异，且设施菜地土壤中nirK型反硝化细菌的群落结构和丰度与大田差异明显.土壤pH值、有机质及硝酸盐含量均影响nirK型反硝化细菌的群落结构和丰度.系统发育分析结果表明，土壤中除存在与厌氧反硝化细菌亲缘相近的nirK型反硝化微生物外，还存在与好氧反硝化菌亲缘关系相近的nirK型反硝化微生物，如根瘤菌属、苍白杆菌属、土壤杆菌属等.

Abstract: The community structure and abundance of nirKtype denitrifying bacteria in different soil layers (0-20 cm and 20-40 cm) under various fertilization regimes in Wuwei, Gansu Province were investigated by the combination of terminal restriction fragment length polymorphism (T-RFLP) and realtime quantitative PCR. Results showed that the nirKtype denitrifying bacteria community structure was significantly affected by fertilization regimes, especially for 70, 156 and 190 bp T-RFs that represented the dominant populations in greenhouse soil. Fertilization regimes significantly influenced the abundance of nirK gene in the 0-20 cm soil layer with the highest abundance of nirK gene copy number (2.16×107 copies·g^-1 soil) detected in the manure treatment (M), which was 2.04 and 2.02 times of that in the control (CK) and chemical fertilizer (NPK) treatments, respectively. Both the dominant population and abundance of nirKtype denitrifying bacteria in the greenhouse soil were significantly different between the 0-20 cm and 20-40 cm soil layers, and the nirKtype denitrifying bacteria community structure and abundance in the greenhouse soil were obviously different from that in the field. Soil pH, soil organic matter content and nitrateN content had the greatest influence on the bacterial community composition. Phylogenetic
analysis indicated that there were not only anaerobic nirKtype denitrifying bacteria in greenhouse soil, but also aerobic denitrifying bacteria, such as Rhizobium, Ochrobactrum, Agrobacterium.

曾希柏1**,王亚男1,王玉忠2,白玲玉1,李莲芳1,段然1,苏世鸣1,吴翠霞1. 施肥对设施菜地nirK型反硝化细菌群落结构和丰度的影响[J]. 应用生态学报.

ZENG Xi-bai1, WANG Ya-nan1, WANG Yu-zhong2, BAI Ling-yu1, LI Lian-fang1, DUAN Ran1, SU Shi-ming1, WU Cui-xia1. Effects of different fertilization regimes on abundance and community structure of the nirKtype denitrifying bacteria in greenhouse vegetable soils.[J]. Chinese Journal of Applied Ecology.