Abstract: We detected absorption and transportation of ions in the leaves of Atriplex nummularia, Atriplex canescens and Lycium barbarum under NaHCO₃ stress (300 mmol·L^-1) by using atomic absorption spectrophotometry and non-invasive ion flux measurement. The results showed that leaves of the A. nummularia, A. canescens and L. barbarum exhibited a high capacity to induce the Na⁺ accumulation when compared with that of control. The higher the concentration of NaHCO₃ treatment, the more Na⁺  accumulated in the leaves of the three plants under experimental condition. L. barbarum showed a higher Na⁺ efflux in the mesophyll cells, whereas A. nummularia and A. canescens showed a relative lower efflux. A lower K⁺ content and a higher Na⁺/K⁺  ratio were detected in leaves of A. nummularia and L. barbarum. However, a higher K⁺ content and a lower Na⁺/K⁺ ratio were seen in leaves of A. canescens. Due to induction of Ca²⁺ efflux under the NaHCO₃ treatment, a lower Ca²⁺ content and a higher Na⁺/Ca²⁺ratio were observed in L. barbarum. On the contrary, a higher Ca²⁺ influx was observed in A. nummularia and A. canescens. These results suggested that the three shrubs species had different Na⁺ segmentation strategies. The accumulation of Na⁺ inhibited Ca²⁺ absorption in leaves of L. barbarum, while in the A. nummularia and A. canescens, Ca²⁺ influx induced \[Ca²⁺\]cyt which preserved a lessdepolarized PM and then inhibited K efflux. The maintaining of cellular K⁺/Na⁺ homeostasis in A.nummularia and A. canescens might be achieved by the induction of \[Ca²⁺\]cyt under the NaHCO₃ treatment.