Abstract: The water consumption, water consumption rate, net photosynthetic rate (P_n), transpiration rate (T_r) and water use efficiency (WUE) of Eucalyptus urophylla × E. grandis DH33-27, E. urophylla × E. grandis DH32-29, E. grandis H1, Corymbia ptychocarpa and an indigenous tree species Bischofia javanica were studied in normal soil moisture condition. The average daily water consumption of the five tree species was in the order of E. urophylla × E. grandis DH32-29(188.47±14.91) g > E. urophylla × E. grandis DH33-27(169.27±16.26) g > E. grandis H1 (118.65±5.32) g > B. javanica (38.12±1.46) g > C. ptychocarpa (20.13±1.72) g, which had obviously positive correlation with the total leaf area of each seedling. The water consumption was mostly in the daytime, which took 90% of the whole day water consumption. The daily change of the water consumption rates of 5 kinds of seedlings followed the curve with one peak at 12:00-14:00. The total water consumption ability of Eucalyptus and Corymbia was higher than that of B. javanica. The water consumption rate of C. ptychocarpa was far higher than that of the other 4 kinds of seedlings, so its large area planting should be given full consideration to this issue. The change in water consumption rate of E. urophylla × E. grandis DH33-27 was mostly impacted by environmental temperature and humidity, because its water consumption rate was the smallest among 4 kinds of Eucalyptus and Corymbia seedlings during the daytime with high temperature and low humidity. The four clone seedlings of Eucalyptus and Corymbia showed higher photosynthetic rates and transpiration rates compared with B. javanica. Two clone seedlings of E. urophylla × E. grandis had better watersaving performance. The WUE of  Eucalyptus and Corymbia was higher than that of B. javanica in general, except E. urophylla × E. Grandis DH33-27.