Abstract: A total of twentythree endophytic bacteria were isolated from Tripterygium wilfordii, among which three strains were selected for further studying based on their capabilities of growthpromotion and wheat germination. All three isolated strains could produce phytohormone and siderophore, and LG3 and LJ10 strains were capable of dissolving phosphorus. Additionally, LG3 and LY4 strains were both proved to have nitrogenfixing function and 1-aminocyclopropane-1-carboxylate deaminase activity. Bacterial 16S rDNA sequence identification and homology analysis suggested that LG3 and LY4 strains belonged to the Enterobacter and LJ10 belonged to the Pantoea. The results of reinoculation experiment demonstrated that three endophytic bacteria could not only promote the growth of Tripterygium wilfordii, but also improve the triptolide contents of different organs significantly.