接种变栖克雷伯氏菌DX120E对不同甘蔗品种的促生效应

应用生态学报

接种变栖克雷伯氏菌DX120E对不同甘蔗品种的促生效应

魏春燕^1,2,邢永秀^1,2,林丽³,杨丽涛^1,3,4,李杨瑞^1,2,3**,胡春锦⁴

(¹广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室，广西南宁 530004； ²广西作物遗传改良生物技术重点开放实验室，广西南宁 530007；
³中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术与遗传改良重点实验室，广西南宁 530007； ⁴广西农业科学院微生物研究所，广西南宁 530007)

出版日期:2014-07-18 发布日期:2014-07-18

Growth-promoting effect of inoculating Klebsiella variicola DX120E on different sugarcane cultivars.

WEI Chun-yan^1,2, XING Yong-xiu^1,2, LIN Li³, YANG Li-tao^1,3,4, LI Yang-rui^1,2,3, HU Chun-jin^4

(¹College of Agriculture, Guangxi University/State Key Laboratory of Conservation and Utilization of Subtropical Agrobioresources, Nanning 530004, China;
²Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning 530007, China； ³Sugarcane Research Center, Chinese Academy of Agricultural Sciences/ Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Nanning 530007, China; ^4Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China)

Online:2014-07-18 Published:2014-07-18

摘要/Abstract

摘要：

为探究具有高固氮酶活性的变栖克雷伯氏菌DX120E回接甘蔗后的固氮能力和促生效应，以甘蔗品种B8和GT21的无菌组培苗为材料，采用根部接种的方法，研究固氮菌DX120E在甘蔗体内的定殖数量及其对甘蔗组培苗植株生长、氮代谢关键酶活性和硝态氮含量及矿质元素吸收的影响.结果表明：固氮菌DX120E能在甘蔗根和地上部分组织内生存和定殖；接种固氮菌DX120E可以有效促进甘蔗植株生长和对矿质营养的吸收；显著提高甘蔗植株体内的硝酸还原酶(NR)活性，同时也能在一定程度上提高植株体内谷氨酰胺合成酶(GS)活性，增加硝态氮含量.表明变栖克雷伯氏菌DX120E对甘蔗具有明显的促生效应，在生物固氮肥开发方面具有较大的应用前景.

Abstract: In order to investigate the growth promoting effect of inoculating Klebsiella variicola DX120E, a bacterial strain with high activity of associative nitrogen fixation, on sugarcane, the strain was inoculated through roots into the pathogen free micropropagated seedlings of two sugarcane cultivars B8 and GT21. The bacterial numbers colonized in sugarcane plants, the activities of the key enzymes for nitrogen metabolism, the nitrate concentration and nutrient uptake were analyzed. The results indicated that the DX120E strain could live, propagate and colonize in the roots and aerial parts of sugarcane seedlings. The DX120E inoculation could effectively promote the plant growth and nutrient uptake, significantly improve the nitrate reductase (NR) activities, and increase the glutamine synthetase (GS) activities and nitrate concentration in certain degree in the leaves, compared with the uninoculated seedlings. It was suggested that Klebsiella variicola DX120E possesses a significant growth promoting effect on sugarcane plants which has a great application potential in developing biological nitrogen fixation fertilizer for sugarcane.

魏春燕1,2,邢永秀1,2,林丽3,杨丽涛1,3,4,李杨瑞1,2,3**,胡春锦4. 接种变栖克雷伯氏菌DX120E对不同甘蔗品种的促生效应[J]. 应用生态学报.

WEI Chun-yan1,2, XING Yong-xiu1,2, LIN Li3, YANG Li-tao1,3,4, LI Yang-rui1,2,3, HU Chun-jin4. Growth-promoting effect of inoculating Klebsiella variicola DX120E on different sugarcane cultivars.[J]. Chinese Journal of Applied Ecology.