四川松茸内生细菌群落结构与多样性

应用生态学报

四川松茸内生细菌群落结构与多样性

李强¹,李小林²,黄文丽³,熊川¹,杨阳¹,杨志荣¹,郑林用^1,4**

(¹四川大学生命科学学院，成都 610065； ²四川省农业科学院土壤肥料研究所，成都 610066； ^3四川省农业科学院生物技术核技术研究所，成都 610066； ⁴四川省农业科学院，成都 610066)

出版日期:2014-11-18 发布日期:2014-11-18

Community structure and diversity of entophytic bacteria in Tricholoma matsutake in Sichuan Province, Southwest China.

LI Qiang¹, LI Xiao-lin², HUANG Wen-li³, XIONG Chuan¹, YANG Yang¹, YANG Zhi-rong¹, ZHENG Lin-yong^1,4

(¹College of Life Sciences, Sichuan University, Chengdu 610065, China; ^2Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; ^3Institute of Biological & Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; ^4Sichuan Academy of Agricultural Sciences, Chengdu 610066, China)

Online:2014-11-18 Published:2014-11-18

摘要/Abstract

摘要：

为了解松茸的内生细菌群落结构与多样性，以采自四川7个松茸主产区的14个松茸样品为材料，通过变性梯度凝胶电泳(DGGE)分析其内生细菌的群落结构与多样性差异.结果表明：各个产区松茸样品的内生细菌群落结构与多样性存在明显差异.但采自相近地点、相似环境的松茸样品内生细菌群落结构相似性较高；样品的丰度范围为15～25，多样性指数依次为盐源＞冕宁＞会东＞木里＞雅江＞盐边＞小金；条带回收测序构建的系统发育树显示，松茸内生细菌种群丰富，各个产区松茸优势种群存在一定的差异，但假单胞菌属、爱文菌属、芽孢杆菌属在所有样品中均有分布，而且都占据一定的优势地位.产碱杆菌属与鞘氨醇杆菌属在大部分样品中为优势种群；而杜擀氏菌属、赖氨酸芽孢杆菌属在特定样品中为优势种群.表明松茸内生细菌具有丰富的多样性，研究结果可为筛选促进松茸生长的内生菌提供依据.

Abstract: In order to understand the diversity and community structure of endophytic bacteria in Tricholoma matsutake, 14 Tricholoma matsutake samples were collected from 7 main production counties of Xiaojin, Yajiang, Muli, Yanyuan, Yanbian, Huidong and Mianning. The entophytic bacterial community structure and diversity were investigated by PCR denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that the diversity and community structure of endophytic bacteria in T. matsutake varied significantly, while those from the environments alike had high similarity. In addition, each T. matsutake sample contained more than 15 species of endophytes, with that from Yanyuan having the highest endophytes diversity index and abundance, while those from Xiaojin were the lowest. Phylogenetic tree showed that endophytic bacterial species in T. matsutake were abundant. Dominant bacterial population in T. matsutake collected from different places varied, and Pseudomonas, Ewingella and Bacillus were distributed in all samples and retained a certain advantage. Alcaligenes and Sphingobacterium distributed in most samples, while Duganella and Lysinibacillus only in certain ones. This study demonstrated that the endophytic bacteria in T. matsutake were abundant and diversified, which could be in favor of searching the dominant bacteria promoting the growth of T. matsutake. 

李强1,李小林2,黄文丽3,熊川1,杨阳1,杨志荣1,郑林用1,4**. 四川松茸内生细菌群落结构与多样性[J]. 应用生态学报.

LI Qiang1, LI Xiao-lin2, HUANG Wen-li3, XIONG Chuan1, YANG Yang1, YANG Zhi-rong1, ZHENG Lin-yong1,4. Community structure and diversity of entophytic bacteria in Tricholoma matsutake in Sichuan Province, Southwest China.[J]. Chinese Journal of Applied Ecology.