耕作方式对黑土表层土壤微生物生物量碳的影响

应用生态学报

耕作方式对黑土表层土壤微生物生物量碳的影响

孙冰洁^1,2,贾淑霞¹,张晓平^1**,梁爱珍¹,陈学文¹,张士秀¹,刘四义^1,2,陈升龙^1,2

(¹中国科学院东北地理与农业生态研究所中国科学院黑土区农业生态重点实验室, 长春 130102; ²中国科学院大学, 北京 100049)

出版日期:2015-01-18 发布日期:2015-01-18

Impact of tillage practices on microbial biomass carbon in top layer of black soils.

SUN Bing-jie^1,2, JIA Shu-xia¹, ZHANG Xiao-ping¹, LIANG Ai-zhen¹, CHEN Xue-wen¹, ZHANG Shi-xiu¹, LIU Si-yi^1,2, CHEN Sheng-long^1,2

(¹Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; ²University of Chinese Academy of Sciences, Beijing 100049, China)

Online:2015-01-18 Published:2015-01-18

摘要/Abstract

摘要：

以保护性耕作13年田间定位试验玉米-大豆轮作黑土为研究对象,探讨了玉米不同生长期(2013年6月至次年4月)0～5、5～10和10～20 cm土层土壤微生物生物量碳(MBC)对耕作方式响应的动态变化.土壤MBC含量采用氯仿熏蒸法测定.结果表明: 土壤MBC含量受时间和土层影响显著.免耕和垄作下土壤MBC含量表现为4月最低,各土层最高值多出现在8月,而秋翻各土层土壤MBC含量也表现为4月最低,但最高值出现在6月.同一采样时间下,耕作方式仅显著影响表层0～5 cm的MBC含量,免耕和垄作下土壤MBC含量出现明显的分层现象,表现为在表层0～5 cm富集.各采样时间下免耕和垄作土壤层化率(0～5 cm/10～20 cm)均显著高于秋翻,9月的土壤层化率增幅最大,为67.8%和95.5%.综上,时间和土层显著影响土壤MBC,而耕作方式主要影响表层土壤MBC的积累与分布.免耕和垄作下表层土壤MBC的积累明显.

Abstract: A study was conducted on a longterm (13 years) tillage and rotation experiment on black soil in northeast China to determine the effects of tillage, time and soil depth on soil microbial biomass carbon (MBC). Tillage systems included no tillage (NT), ridge tillage (RT) and mouldboard plough (MP). Soil sampling was done at 0-5, 5-10 and 10-20 cm depths in June, August and September, 2013, and April, 2014 in the corn phase of cornsoybean rotation plots. MBC content was measured by the chloroform fumigation extraction (CFE) method. The results showed that the MBC content varied with sampling time and soil depth. Soil MBC content was the lowest in April for all three tillage systems, and was highest in June for MP, and highest in August for NT and RT. At each sampling time, tillage system had a significant effect on soil MBC content only in the top 0-5 cm layer. The MBC content showed obvious stratification under NT and RT with a higher MBC content in the top 0-5 cm layer than under MP. The stratification ratios under NT and RT were greatest in September when they were respectively 67.8% and 95.5% greater than under MP. Our results showed that soil MBC contents were greatly affected by the time and soil depth, and were more apparently accumulated in the top layer under NT and RT.

孙冰洁1,2,贾淑霞1,张晓平1**,梁爱珍1,陈学文1,张士秀1,刘四义1,2,陈升龙1,2. 耕作方式对黑土表层土壤微生物生物量碳的影响[J]. 应用生态学报.

SUN Bing-jie1,2, JIA Shu-xia1, ZHANG Xiao-ping1, LIANG Ai-zhen1, CHEN Xue-wen1, ZHANG Shi-xiu1, LIU Si-yi1,2, CHEN Sheng-long1,2. Impact of tillage practices on microbial biomass carbon in top layer of black soils.[J]. Chinese Journal of Applied Ecology.