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油桃花芽破眠过程中H2O2代谢与Ca2+转运的关系

谭钺1,2,高东升2**,李玲2,魏海蓉1,王甲威1,刘庆忠1   

  1. (1山东省果树研究所/山东省果树生物技术育种重点实验室, 山东泰安 271000; 2山东农业大学园艺科学与工程学院, 山东泰安 271018)
  • 出版日期:2015-02-18 发布日期:2015-02-18

Relationships between H2O2 metabolism and Ca2+ transport in dormancybreaking process of nectarine floral buds.

TAN Yue1,2, GAO Dong-sheng2, LI Ling2, WEI Hai-rong1, WANG Jia-wei1, LIU Qing-zhong1   

  1. (1Shandong Institute of Pomology/Shandong Key Laboratory of Fruit Tree Biotechnology Breeding, Tai’an 271000, Shandong, China; 2College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China)
  • Online:2015-02-18 Published:2015-02-18

摘要: 利用化学测定法分析高温、单氰胺和TDZ 3种破眠处理对“曙光”油桃休眠花芽H2O2代谢的主要影响,利用非损伤微测技术检测H2O2对休眠芽Ca2+转运的影响,研究H2O2在芽休眠解除过程中的调控作用.结果表明: 在深休眠时期,高温和单氰胺处理均能诱导芽内H2O2含量升高和过氧化氢酶(CAT)活性降低,并具有显著的破眠作用;TDZ对H2O2含量及CAT、过氧化物酶(POD)活性影响不大,破眠效果较差.休眠花芽原基组织钙通道活跃,对外源Ca2+呈吸收状态.外源H2O2可诱导休眠花芽原基组织Ca2+转运发生变化,低浓度H2O2降低Ca2+吸收速率,高浓度H2O2使组织对Ca2+的转运由吸收转变为释放.这表明休眠芽内H2O2信号和Ca2+信号相关联,通过诱导H2O2积累调控Ca2+信号可能在高温和单氰胺打破休眠的信号转导过程中起重要作用.

Abstract: In order to explore regulatory function of H2O2 in bud dormancy release, main effects of three dormancybreaking treatments (high temperature, hydrogen cyanamide and TDZ) on H2O2 metabolism were determined, and impacts of H2O2 on Ca2+ transport were tested using noninvasive microtest technique. The results showed that both high temperature and hydrogen cyanamide induced H2O2 accumulation and CAT inhibition were efficient in breaking dormancy during deep dormancy period. However, TDZ showed little impacts on H2O2 metabolism and was much less effective in breaking dormancy. Dormant floral primordium was absorbing state to exogenous Ca2+ due to active calcium channels. The Ca2+ transport could be changed by exogenous H2O2. H2O2 of low concentration reduced the absorption rate of Ca2+, and  at high concentration, it changed the Ca2+ transport direction from absorption to release. The results indicated that H2O2 signals were related with Ca2+ signals in dormant buds. Ca2+ signal regulated by H2O2 accumulation might be important in the dormancybreaking signal transduction process induced by high temperature and hydrogen cyanamide.