Abstract: In this study, a pot experiment was conducted with “Wandao 205” as test materials to investigate Se uptake and translocation in rice  under different Se concentrations (0.5, 1.0, and 1.5 mg·kg^-1). Results showed that there was no significant change in rice yield when Se concentration in soil was lower than 1.5 mg·kg^-1. Significant linear correlations existed between Se concentration in soil and different rice plant tissues. Se concentration in rice plant followed the order of root > straw > grain. Se concentration in different rice grain fractions followed the order of bran > polished rice > hull. The root absorption index of Se was more than 1.86, suggest that the rice could absorpt Se from soil effectively. However, the transport and accumulation of Se in seeds from Seenriched soil was relatively constant. The Se transport index in seeds was between 0.53 and 0.59. Soil Se concentration within the range of 0.5 to 1.0 mg·kg^-1 could produce Seenriched rice, which might be enough for human requirement of 60-80 μg·d^-1 Se. However, polished rice at highSe treatment (1.5 mg·kg^-1) exceeded the maximum standard limit of Se (0.3 mg·kg^-1) for cereals in China. These results suggested that we could produce Seenriched rice under soil Se concentration in the range of 0.5 to 1.0 mg·kg^-1 without spraying Se fertilizer, thus reducing the cost and avoiding soil and water pollution caused by exogenous Se.