• 研究报告 •

### 低温条件下拔节期小麦叶片内源激素含量和抗氧化酶活性的变化

1. (扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心， 江苏扬州 225009)
• 出版日期:2015-07-18 发布日期:2015-07-18

### Changes of endogenous hormone contents and antioxidative enzyme activities in wheat leaves under low temperature stress at jointing stage.

LI Chun-yan, XU Wen, LIU Li-wei, YANG Jing, ZHU Xin-kai, GUO Wen-shan

1. (Jiangsu National Key Laboratory Nurturing Center of Crop Genetics and Physiology/CoInnovation Center for Modern Production Technology of Grain Crops, Yangzhou
University, Yangzhou 225009, Jiangsu, China)
• Online:2015-07-18 Published:2015-07-18

Abstract: Low temperature stresses (-3 and -5 ℃) were simulated using artificial temperaturecontrolled phytotrons to study the freezing rate, the contents of endogenous hormones, and the activities of antioxidative enzymes in the leaves of wheat plants of Yangmai 16 (YM 16) and Xumai 30 (XM 30) at jointing stage. The grade and index of freezing injury increased with lower temperature and longer stress. The freezing rate was at the 5th level and the main stems and tillers of both cultivars were finally dead under -5 ℃ lasting for 72 h. On the last day of stress initiation, the contents of abscisic acid (ABA) and zeatin riboside (ZR), and the activities of superoxide dismutase (SOD), peroxide dismutase (POD), and catalase (CAT) in leaves increased at the beginning and then declined as low temperature progressed. On the 3rd day after stress, the contents of ABA and ZR and the activities of antioxidative enzymes were higher than those on the last day of cold stress, and then reduced to the level of the control on the 6th day after stress. The content of gibberellins (GA3) was lowered by cold stress. For YM 16, GA3 content increased from the 3rd day to the 6th day after cold stress, whereas, for XM 30, it increased first and then decreased. For the treatment of -5 ℃ lasting for 72 h, the contents of hormones and the activities of antioxidative enzymes were significantly lower than those of the other treatments. Correlation analyses showed that higher ABA and ZR contents,  and higher SOD, POD and CAT activities as well as lower GA3 content could alleviate the low-temperature injury in wheat plants under low temperature stress.