外源糖浸种缓解盐胁迫下玉米种子萌发

应用生态学报

外源糖浸种缓解盐胁迫下玉米种子萌发

赵莹¹,杨克军^1,2,李佐同^1,2**,赵长江^1,2,徐晶宇^1,2,胡雪微¹,石新新¹,马丽峰¹ 

(¹黑龙江八一农垦大学农学院, 黑龙江大庆 163319; ^2黑龙江省教育厅寒地作物种质改良与栽培重点实验室, 黑龙江大庆 163319)

出版日期:2015-09-18 发布日期:2015-09-18

Alleviation of salt stress during maize seed germination by presoaking with exogenous sugar.

ZHAO Ying¹, YANG Ke-jun^1,2, LI Zuo-tong^1,2, ZHAO Chang-jiang^1,2, XU Jing-yu^1,2, HU Xue-wei¹, SHI Xin-xin¹, MA Li-feng^1

(¹College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China; ^2Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions of Heilongjiang Province Education Department, Daqing 163319, Heilongjiang, China)

Online:2015-09-18 Published:2015-09-18

摘要/Abstract

摘要： 以玉米品种‘垦玉6号’为材料,在150 mmol·L^-1NaCl胁迫条件下,研究葡萄糖(Glc)和蔗糖(Suc)浸种对玉米种子萌发阶段耐盐性的影响.结果表明: 盐胁迫下，0.5 mmol·L^-1 Glc、Suc浸种可促进玉米种子萌发及幼苗早期生长,其中Glc浸种玉米胚芽和胚根长及相应干质量增加到盐处理的1.5、1.3、2.1、1.8倍;Suc浸种玉米分别增加到1.7、1.3、2.7、1.9倍;盐胁迫下Glc、Suc浸种可减少胚芽中硫代巴比妥酸反应物(TBARS)和过氧化氢(H2O2)含量,与盐处理相比分别降低24.9%、20.6%;Glc、Suc浸种可显著提高盐胁迫下玉米胚芽超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽过氧化物酶(GPX)、谷胱甘肽还原酶(GR)的活性,并诱导葡萄糖6磷酸脱氢酶(G6PDH)活性的升高,其中Glc浸种玉米SOD、APX、GPX、GR、G6PDH活性较盐处理分别提高66.2%、62.9%、32.0%、38.5%、50.5%,Suc浸种玉米较盐处理分别提高67.5%、59.8%、30.0%、38.5%、50.4%;Glc、Suc浸种胚芽中抗坏血酸 (ASA)、谷胱甘肽(GSH)含量及ASA/DHA、GSH/GSSG显著提高,其中G6PDH活性与外源糖诱导的较强的抗氧化能力密切相关.Glc、Suc浸种还可提高盐胁迫下玉米胚芽中K⁺/Na⁺,分别为盐处理的2.3、2.4倍.外源 Glc、Suc浸种可通过提高玉米种子抗氧化能力及维持体内K⁺和Na⁺离子平衡缓解盐胁迫对玉米种子萌发的抑制效应.

Abstract: The maize variety Kenyu 6 was used to study the effects of exogenous glucose (Glc) and sucrose (Suc) on salt tolerance of maize seeds at germination stage under 150 mmol·L^-1 NaCl treatment. Results showed that under salt stress condition, 0.5 mmol·L^-1 exogenous Glc and Suc presoaking could promote seed germination and early seedling growth. Compared with the salt treatment, Glc presoaking increased the shoot length, radicle length and corresponding dry mass up to 1.5, 1.3, 2.1 and 1.8 times, and those of the Suc presoaking treatment increased up to 1.7, 1.3. 2.7 and 1.9 times, respectively. Exogenous Glc and Suc presoaking resulted in decreased levels of thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H₂O₂) content of maize shoot under salt stress, which were lowered by 24.9% and 20.6% respectively. Exogenous Glc and Suc presoaking could increase the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione peroxidase (GPX), glutathione reductase (GR) and induce glucose6phosphate dehydrogenase (G6PDH) activity of maize shoot under salt stress. Compared with the salt treatment, Glc presoaking increased the activity of SOD, APX, GPX, GR and G6PDH by 66.2%, 62.9%, 32.0%, 38.5% and 50.5%, and those of the Suc presoaking increased by 67.5%, 59.8%, 30.0%, 38.5% and 50.4%, respectively. Glc and Suc presoaking also significantly increased the contents of ascorbic acid (ASA) and glutathione (GSH), ASA/DHA and GSH/GSSG. The G6PDH activity was found closely related with the strong antioxidation capacity induced by exogenous sugars. In addition, Glc and Suc presoaking enhanced K⁺/Na⁺ in maize shoot by 1.3 and 1.4 times of water soaking salt treatment, respectively. These results indicated that exogenous Glc and Suc presoaking could improve antioxidation capacity of maize seeds and maintain the in vivo K⁺/Na⁺ ion balance to alleviate the inhibitory effect of salt stress on maize seed germination.

赵莹1,杨克军1,2,李佐同1,2**,赵长江1,2,徐晶宇1,2,胡雪微1,石新新1,马丽峰1 . 外源糖浸种缓解盐胁迫下玉米种子萌发[J]. 应用生态学报.

ZHAO Ying1, YANG Ke-jun1,2, LI Zuo-tong1,2, ZHAO Chang-jiang1,2, XU Jing-yu1,2, HU Xue-wei1, SHI Xin-xin1, MA Li-feng1. Alleviation of salt stress during maize seed germination by presoaking with exogenous sugar.[J]. Chinese Journal of Applied Ecology.