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集约经营对毛竹林土壤固氮细菌群落结构和丰度的影响

何冬华,陈俊辉,徐秋芳**,沈秋兰,李永春,毛新伟,程敏   

  1. (浙江农林大学环境与资源学院浙江省森林生态系统碳循环与固碳减排重点实验室,  浙江临安 311300)
  • 出版日期:2015-10-18 发布日期:2015-10-18

Effects of intensive management on abundance and composition of soil N2fixing bacteria in Phyllostachys heterocycla stands.

HE Dong-hua, CHEN Jun-hui, XU Qiu-fang, SHEN Qiu-lan, LI Yong-chun, MAO Xin-wei, CHENG Min   

  1. (Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A&F University, Lin’an 311300, Zhejiang, China)
  • Online:2015-10-18 Published:2015-10-18

摘要:

为揭示集约经营对毛竹林土壤固氮细菌群落特征的影响,采用变性梯度凝胶电泳(DGGE)和荧光定量PCR技术,分析集约经营0(CK)、10、15、20、25年毛竹林土壤固氮菌群落结构和丰度的变化规律,并探讨了影响土壤固氮菌群落的主要环境因素.结果表明: 毛竹林集约经营导致土壤pH下降而速效养分积累;集约经营初期(10年)和后期(25年)土壤固氮细菌的群落结构与对照相似,而中期的15年和20年则与对照差异较大.固氮菌多样性指数和丰度均呈现先减少后增加的趋势,经营15年时达到最小值;土壤固氮细菌表现出对集约经营干扰的抵抗和恢复反应.冗余分析表明,土壤速效钾、水解氮、硝态氮和铵态氮的含量与固氮菌群落结构的变化有较强的相关性,表明集约经营措施导致了土壤固氮细菌短期的变化,但长期而言,不会对土壤固氮细菌产生不良影响.
 

Abstract: Denaturing gradientgel electrophoresis and realtime quantitative PCR (qPCR) were employed to determine the effects of intensive management on soil N2fixing bacteria in a moso bamboo (Phyllostachys heterocycla) plantation. Soil samples were collected from the moso bamboo stands receiving 0 (CK), 10, 15, 20, and 25 years of intensive management. It was found that intensive management caused a strong decrease in soil pH but a general increase in soil available nutrients. The structure of the N2fixing bacterial communities in the soils having received 10 and 25 years of intensive management were quite similar to that from the CK; however, those from 15 and 20 years of intensification differed from the CK. With increasing time of intensive management, the abundance and diversity of the nifH gene at first decreased and then increased, with the minimum values being observed after 15 years of intensive management, indicating the eventual resiliency of N2fixing bacteria to disturbance induced by intensive management. Redundancy analysis indicated that soil available potassium, available nitrogen, nitrite nitrogen, and ammonium nitrogen were more closely related to the changes of N2fixing bacterial community structure compared with the other soil indices measured. In conclusion, the soil N2fixing bacterial community was negatively affected by intensive management in the short term, but could recover in the long term.