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北方温带森林不同海拔梯度土壤碳矿化速率及酶动力学参数温度敏感性

樊金娟1,李丹丹1,2,张心昱2*,何念鹏2,部金凤2,王情3,孙晓敏2,温学发2   

  1. (1沈阳农业大学生物科学技术学院, 沈阳 110866; 2中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101; 3安徽农业大学资源与环境学院, 合肥 230036)
  • 出版日期:2016-01-18 发布日期:2016-01-18

Temperature sensitivity of soil organic carbon mineralization and βglucosidase enzyme kinetics in the northern temperate forests at different altitudes, China.

FAN Jin-juan1, LI Dan-dan1,2, ZHANG Xin-yu2*, HE Nian-peng2, BU Jin-feng2, WANG Qing3, SUN Xiao-min2, WEN Xue-fa2   

  1. (1College of Biological Science and Technology, Shenyang Agricultural University, Shenyang
     110866, China; 2Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; 3School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)
  • Online:2016-01-18 Published:2016-01-18

摘要: 采集长白山脉龙岗支脉老秃顶子南坡3个不同海拔梯度森林(岳桦林、针阔混交林、红松林)土壤,进行室内温度梯度培养试验,研究土壤碳矿化速率(Cmin)和土壤β-1,4-葡萄糖苷酶(βG)动力学参数及温度敏感性.结果表明: 海拔和温度对Cmin均有显著影响,3种森林土壤Cmin均随着培养温度升高而增加,且岳桦林土壤Cmin最高.3种森林土壤碳矿化速率温度敏感性\[Q10(Cmin)\]大小为岳桦林>红松林>针阔混交林,但差异不显著.3种森林土壤βG动力学参数最大反应速率(Vmax)和米氏常数(Km)均随培养温度升高而增加,Vmax的温度敏感性\[Q10(Vmax)\]为1.78~1.90,Km的温度敏感性\[Q10(Km)\]为1.79~2.00.岳桦林Q10(Vmax)/Q10(Km)值显著高于红松林和针阔混交林,表明高海拔岳桦林土壤有机碳水解酶动力学参数受温度升高影响最大.

Abstract: Soil samples, which were collected from three typical forests, i.e., Betula ermanii forest, coniferous mixed broadleaved forest, and Pinus koraiensis forest, at different altitudes along the southern slope of Laotuding Mountain of Changbai Mountain range in Liaoning Province of China, were incubated over a temperature gradient in laboratory. Soil organic carbon mineralization rates (Cmin), soil β-1,4-glucosidase (βG) kinetics and their temperature sensitivity (Q10) were measured. The results showed that both altitude and temperature had significant effects on Cmin. Cmin increased with temperature and was highest in the B. ermanii forest. The temperature sensitivity of Cmin〖KG*3〗\[Q10(Cmin)\] ranked in order of B. ermanii forest > P. koraiensis forest > coniferous mixed broadleaved forest, but did not differ significantly among the three forests. Both the maximum activity (Vmax) and the Michaelis constant (Km) of the βG responded positively to temperature for all the forests. The temperature sensitivity of Vmax〖KG*3〗\[Q10(Vmax)\] ranged from 1.78 to 1.90, and the temperature sensitivity of Km〖KG*3〗\[Q10(Km)\] ranged from 1.79 to 2.00. The Q10(Vmax)/Q10(Km) ratios were significantly greater in the B. ermanii soil than in the other two forest soils, suggesting that the βG kineticsdependent impacts of the global warming or temperature increase on the decomposition of soil organic carbon were temperature sensitive for the forests at the higher altitudes.